Results 41 to 50 of about 94,605 (314)

One-step purification and characterization of alginate lyase from a clinical Pseudomonas aeruginosa with destructive activity on bacterial biofilm [PDF]

Iranian Journal of Basic Medical Sciences, 2017
Objective(s): Pseudomonas aeruginosais a Gram-negative and aerobic rod bacterium that displays mucoid and non-mucoid phenotype. Mucoid strains secrete alginate, which is the main agent of biofilms in chronic P. aeruginosa infections, show high resistance
Parinaz Ghadam, Fatemeh Akhlaghi, Ahya Abdi Ali   +2 more
doaj   +1 more source

Ratoon Season Rice Reduces Methane Emissions by Limiting Acetic Acid Transport to the Rhizosphere and Inhibiting Methanogens

Advanced Science, EarlyView.
This study finds that the interaction between ABA‐OsCIPK2‐OsSWEET1A reduces the allocation of methane producing bacteria carbon source (acetic acid) content to the rhizosphere soil of ratoon season rice, thereby reducing methane emissions. Abstract Rice paddies are a major, persistent source of atmospheric methane (CH4), emission rates depend on the ...
Jingnan Zou, Hailong Xu, Bin Qin, Chaojie Lan, Jinying Li, Bianhong Zhang, Huabin Zhang, Chunlin Guo, Hongfei Chen, Zhongming Fang, Quanzhi Zhao, Wenfei Wang, Changxun Fang, Zhixing Zhang, Wenxiong Lin   +14 more
wiley   +1 more source

Alginate Lyase from Indonesian Bacillus megaterium S245 Shows Activities Toward Polymannuronate and Polyguluronate

Squalen, 2017
Screening of alginate lyase producing bacteria associated with seaweed Sargassum crassifolium was carried out, and isolate S245, identified as Bacillus megaterium S245 was found to produce high alginate lyase activity.
Subaryono Subaryono, Yuwanita Ardilasari, Rosmawaty Peranginangin, Fransisca Rungkat Zakaria, Maggy Thenawidjaja Suhartono   +4 more
doaj   +1 more source

Protein Language Model‐Guided Engineering of a 2,3‐Butanediol Dehydrogenase for the Enantioselective Synthesis of Cyclic α‐Hydroxy Ketones

Advanced Science, EarlyView.
A (2R,3R)‐butanediol dehydrogenase from Bacillus subtilis (BsBDH) is engineered for the enantioselective synthesis of 2‐hydroxycyclohexanone. A PASS computational design strategy is proposed to enhance the thermostability of BsBDH. Moreover, ESM‐1v combined with ISM is utilized for enhancing and inverting its stereoselectivity.
Haote Ding, Ling Jiang, Yijia Song, Zhongji Pu, Lirong Yang, Haoran Yu   +5 more
wiley   +1 more source

Cloning and expression of isocitrate lyase from human round worm Strongyloides stercoralis

Parasite, 2000
A full length cDNA (1463 bp) encoding isocitrate lyase (EC 4.1.3.1) of Strongyloides stercoralis is described. The nucleotide sequence of this insert identified a cDNA coding for the isocitrate lyase.
Siddiqui A.A., Stanley C.S., Berk S.L.
doaj   +1 more source

Two Novel S‐methyltransferases Confer Dimethylsulfide Production in Actinomycetota

Advanced Science, EarlyView.
This study identifies two novel S‐adenosine‐methionine‐dependent methyltransferases, MddM1 and MddM2, in actinomycetes from the Mariana Trench. These enzymes can convert toxic hydrogen sulfide (H2S) and methanethiol (MeSH) into dimethylsulfide (DMS), serving as a cellular detoxification and oxidative stress response.
Ruihong Guo, Zihua Guo, Yi Zhou, Yunhui Zhang, Haojin Cheng, Rebecca Devine, Chuang Sun, Ronghua Liu, Yanfen Zheng, Andrew J. Gates, Jonathan D. Todd, Xiao‐Hua Zhang   +11 more
wiley   +1 more source

Biochemical Characterization of a Bifunctional Enzyme Constructed by the Fusion of a Glucuronan Lyase and a Chitinase from Trichoderma sp.

Life, 2020
Bifunctional enzymes created by the fusion of a glucuronan lyase (TrGL) and a chitinase (ThCHIT42) from Trichoderma sp. have been constructed with the aim to validate a proof of concept regarding the potential of the chimera lyase/hydrolase by analyzing ...
Zeineb Baklouti, Cédric Delattre, Guillaume Pierre, Christine Gardarin, Slim Abdelkafi, Philippe Michaud, Pascal Dubessay   +6 more
doaj   +1 more source

Identification and Characterization of a New Cold-Adapted and Alkaline Alginate Lyase TsAly7A from Thalassomonas sp. LD5 Produces Alginate Oligosaccharides with High Degree of Polymerization [PDF]

, 2022
Chengying Yin, Jiaxia Sun, Hainan Wang, Wengong Yu, Feng Han   +4 more
openalex   +1 more source

Cancer Stem Cells Shift Metabolite Acetyl‐Coenzyme A to Abrogate the Differentiation of CD103+ T Cells

Advanced Science, EarlyView.
Lei et al. demonstrate that cancer stem cells (CSCs) play a pivotal role in impairing the differentiation of CD103+ T cells in patients with non‐small‐cell lung cancer. The key mechanism involves CSC‐derived acetyl‐CoA, which disrupts CD103+ T cell differentiation by sequentially inducing acetylation and ubiquitination of the Blimp‐1 protein. Targeting
Jiaxin Lei, Huiyan Ji, Jing Guo, Mengdi Liu, Danhua Su, Yiran Zheng, Lin Xu, Qinghua Cao, Tao Ren, Jun Gui, Zhenke Wen   +10 more
wiley   +1 more source

Isolation and identification of an alginate lyase-producing marine bacterium strain and its enzymological characteristics

浙江大学学报. 农业与生命科学版, 2013
Alginate polysaccharide produced by marine brown algae or certain Gram-negative bacteria is a linear anionic binary copolymer of β-D-mannuronic acid (M) and α-L-guluronic acid (G) residues. Alginate lyase (EC 4.2.2.3; EC 4.2.2.11), also known as alginase
TANG Haiqing, OU Changrong, ZHENG Xiaodong   +2 more
doaj   +1 more source