Results 131 to 140 of about 787,190 (346)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Chemo- and Regioselective Lysine Modification on Native Proteins

Journal of the American Chemical Society, 2018
Site-selective chemical conjugation of synthetic molecules to proteins expands their functional and therapeutic capacity. Current protein modification methods, based on synthetic and biochemical technologies, can achieve site selectivity, but these ...
M. Matos, Bruno L. Oliveira, Nuria Martínez-Sáez, A. Guerreiro, P. M. S. D. Cal, Jean B. Bertoldo, M. Maneiro, Elizabeth Perkins, Julie A. Howard, M. Deery, J. Chalker, F. Corzana, Gonzalo Jiménez-Osés, Gonçalo J. L. Bernardes   +13 more
semanticscholar   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

Beilstein Journal of Nanotechnology, 2019
Polymer hydrogels are ideal scaffolds for both tissue engineering and drug delivery. A great advantage of poly(amino acid)-based hydrogels is their high similarity to natural proteins. However, their expensive and complicated synthesis often limits their
Dávid Juriga, Evelin Sipos, Orsolya Hegedűs, Gábor Varga, Miklós Zrínyi, Krisztina S. Nagy, Angéla Jedlovszky-Hajdú   +6 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Variation in Protein Content and Amino Acids in the Leaves of Grain, Vegetable and Weedy Types of Amaranths

Agronomy, 2013
Malnutrition has affected almost 31% of pre-school children. This paper provides the information of nutritional values (leaf protein, 15 amino acids, biomass and leaf dry matter) of grain, vegetable and weedy types of amaranths (n = 76 accessions ...
Ryo Ohsawa, Shigeki Yoshida, Rita Andini
doaj   +1 more source

Cellular functions and molecular mechanisms of non-lysine ubiquitination

Open Biology, 2019
Protein ubiquitination is of great cellular importance through its central role in processes such as degradation, DNA repair, endocytosis and inflammation.
Amie J. McClellan, Sophie Heiden Laugesen, L. Ellgaard   +2 more
semanticscholar   +1 more source

Analysis of Jmjd6 cellular localization and testing for its involvement in histone demethylation. [PDF]

, 2010
Methylation of residues in histone tails is part of a network that regulates gene expression. JmjC domain containing proteins catalyze the oxidative removal of methyl groups on histone lysine residues.
Böttger, Angelika, Burrells Alison, Andreas Lengeling, Butler, Danica, Hahn, P., Erck, Christian, Wolf, A., Lengeling, A., Bottger, A., Jens Böse, Erck, C., Christopher J Schofield, Wegener, Ivonne, Wegener Ivonne, Böse, Jens, Christian Erck, Angelika Böttger, Ivonne Wegener, Butler Danica, Lengeling Andreas, Böse Jens, Burrells, A., Alexander Wolf, Hahn Phillip, Bose, J., Erck Christian, Böttger Angelika, Danica Butler, Phillip Hahn, Butler, D., Wolf Alexander, Hahn, Phillip, Wolf, Alexander, Schofield, Christopher J, Alison Burrells, Schofield Christopher J., Lengeling, Andreas, Schofield, Christopher J., Wegener, I., Burrells, Alison, Christopher J. Schofield   +40 more
core   +2 more sources

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

Jugular-infused methionine, lysine and branched-chain amino acids does not improve milk production in Holstein cows experiencing heat stress

Animal, 2017
Poor utilization of amino acids contributes to losses of milk protein yield in dairy cows exposed to heat stress (HS). Our objective was to test the effect of essential amino acids on milk production in lactating dairy cows exposed to short-term HS ...
K.R. Kassube, J.D. Kaufman, K.G. Pohler, J.W. McFadden, A.G. Ríus   +4 more
doaj   +1 more source