Results 151 to 160 of about 686,065 (200)

Lysosomal enzyme targeting

Biochemical Society Transactions, 1990
Historical overview I want to express my appreciation to the Biochemical Society for having invited me to deliver the 1989 Jubilee Lecture. It is truly a great honour. The word Jubilee connotes a very special anniversary, and in this spirit I want to impart an historical overview to the Lecture in addition to reviewing my most recent work.
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Lysosomal enzymes in human platelets

Cell Biochemistry and Function, 1985
AbstractIn human freshly prepared platelets the following lysosomal enzymes were studied: α‐mannosidase, α‐fucosidase, β‐galactosidase, β‐glucosidase, β‐glucuronidase, β‐N‐acetylglucosaminidase and acid phosphatase. For each of the examined enzymes the conditions providing maximal activity (pH, buffer), kinetic parameters (saturating substrate ...
G, Leoncini, F, Balestrero, M, Maresca
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Lysosomal enzymes in subretinal fluid

Albrecht von Graefes Archiv f�r Klinische und Experimentelle Ophthalmologie, 1976
Samples of subretinal fluid (SRF) from patients with primary rhegmatogeneous retinal detachment of various duration were studied using standard enzymologic techniques. The protein content in SRF increased with the duration of detachment. The activities of lysosomal enzymes in SRF, which were acid phosphatase, beta-glucuronidase, and cathepsin D, also ...
S, Hayasaka, S, Hara, K, Mizuno
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Lysosomal enzymes in cerebral atrophy

Clinica Chimica Acta, 1976
In seven patients with cerebral atrophy due to pre-senile dementia and/or cerebrovascular disease, the activity of acid phosphatase in lumbar cerebrospinal fluid (CSF) was higher (p less than 0.05) than in six controls. The activity of arylsulphatase and beta-galactosidase in CSF was the same in the two groups.
C M, Yates, A, Urquhart, H, Wilson, D, Davidson, W A, Copland   +4 more
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Antigen catabolism by lysosomal enzymes

Immunochemistry, 1970
Abstract The antigenicity of eight proteins (gelatin, insulin, hemoglobin, egg albumin, human γ-globulin, bovine serum albumin, keyhole limpet hemocyanin (KLH), and tetanus toxin) was compared by quantitative precipitin tests. Degradation of these proteins by lysosomal cathepsins in vitro was measured by estimation of the total amino acids released ...
W L, Ryan, J W, Lee
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Chromatography of lysosomal enzymes on hydroxylapatite

Journal of Chromatography A, 1969
Abstract Soluble proteins were extracted from Triton WR 1339-filled rat liver lysosomes and were chromatographed on hydroxylapatite by stepwise elution with increasing concentrations of phosphate buffer (pH 6.8). The distribution of the activities of the cathepsins A, B, C, and D; β-galactosidase; α-mannosidase; β-glucuronidase; β-N ...
J O, Young, D M, Hanes, A L, Tappel
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Lysosomal sequestration of cytosolic enzymes and lysosomal thiol cathepsins

Advances in Enzyme Regulation, 1985
Cytoplasmic proteins are degraded with different half-lives in vivo. Large parts of proteins are believed to be degraded primarily in autophagic vacuoles-lysosomal system. However, the mechanism by which cell proteins are delivered to lysosomes and whether such a process might be selective for certain cell proteins are still unresolved. We examined the
N, Katunuma, E, Kominami
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Enzyme Replacement for Lysosomal Diseases

Annual Review of Medicine, 2006
Following the demonstration of the nature of the enzymatic defects in the sphingolipid storage disorders in the mid-1960s, consideration was directed to the development of therapy for patients with these conditions. High on the list of possibilities was enzyme supplementation or replacement.
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Inhibition of Lysosomal Enzymes by Dapsone

Archives of Dermatology, 1974
The possible inhibition of lysosomal enzymes by dapsone (diaminodiphenylsulfone) was studied. Experimentally induced hypervitaminosis A in rabbits results in loss of chondroitin sulfate from cartilage matrix through the release of lysosomal enzymes. In this study, thinning and decreased toluidine blue and Alcian blue staining of tracheal and articular
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Biosynthesis of lysosomal enzymes

Trends in Biochemical Sciences, 1980
During their synthesis lysosomal enzymes are subjected to extensive processing, which involves both the protein and the sugar moieties of these molecules. The many modifications to the sugars appear to play a decisive role in sorting those molecules to be delivered into the lysosomes from those to be secreted.
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