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Cold Spring Harbor Protocols, 2006
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Joseph, Sambrook, David W, Russell
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A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Joseph, Sambrook, David W, Russell
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1986
This chapter focuses on a method of sequencing M13 clones that allows the determination of the nucleotide sequence of inserts cloned into M13 vectors. A 17-mer oligonucleotide primer is annealed to the M13 vector in a position adjacent to the polylinker cloning region.
Leonard G. Davis +2 more
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This chapter focuses on a method of sequencing M13 clones that allows the determination of the nucleotide sequence of inserts cloned into M13 vectors. A 17-mer oligonucleotide primer is annealed to the M13 vector in a position adjacent to the polylinker cloning region.
Leonard G. Davis +2 more
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Engineering M13 for phage display
Biomolecular Engineering, 2001Phage display is achieved by fusing polypeptide libraries to phage coat proteins. The resulting phage particles display the polypeptides on their surfaces and they also contain the encoding DNA. Library members with particular functions can be isolated with simple selections and polypeptide sequences can be decoded from the encapsulated DNA.
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Replication of bacteriophage M13
Journal of Molecular Biology, 1977The origin for bacteriophage M13 single-strand synthesis has been localized by two different experimental approaches: (1) localization of the discontinuity in late life-cycle RFII ‡ , one of the initial product molecules during single-strand synthesis, and (2) determination of a gradient of radioactive label in RFI pulselabeled during single-strand ...
Sidney V. Suggs, Dan S. Ray
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Growing Bacteriophage M13 in Liquid Culture
Cold Spring Harbor Protocols, 2017Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Michael R, Green, Joseph, Sambrook
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????????-?????????? ?????????????????????? ???????????????????? ???????? M13
2019???????????????? ????????-?????????? ?????????????????????? ???????? M13mp8 ?? mp9. ?????????????? ???????????????????????????????????? ?????????? ?????????????????????? ???? 14 ???? 34 % ?????????????? ???????????????? ????????????. ?????? ????????-???????? ?????????????????? ?????????????? ?????? ~640 ??. ??., ???????????????????? ????????????????????
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Cloning into Bacteriophage M13 Vectors
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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Replication of bacteriophage M13
Virology, 1971Arleen B. Forsheit, Dan S. Ray
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