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m6A-SAC-seq for quantitative whole transcriptome m6A profiling
Nature Protocols, 2022N6-methyladenosine (m6A) is the most abundant mRNA modification in mammalian cells, regulating many physiological processes. Here we describe a method for base-resolution, quantitative m6A sequencing in the whole transcriptome. The enzyme and small-molecule cofactor used in this protocol are prepared by recombinant protein expression and organic ...
Ruiqi Ge +8 more
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Methods, 2022
With the rapid development of high-throughput sequencing techniques nowadays, extensive attention has been paid to epitranscriptomics, which covers more than 150 distinct chemical modifications to date. Among that, N6-methyladenosine (m6A) modification has the most abundant existence, and it is also significantly related to varieties of biological ...
Zhanmin, Liang +4 more
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With the rapid development of high-throughput sequencing techniques nowadays, extensive attention has been paid to epitranscriptomics, which covers more than 150 distinct chemical modifications to date. Among that, N6-methyladenosine (m6A) modification has the most abundant existence, and it is also significantly related to varieties of biological ...
Zhanmin, Liang +4 more
openaire +2 more sources
m6A readers, writers, erasers, and the m6A epitranscriptome in breast cancer
Journal of Molecular Endocrinology, 2023Epitranscriptomic modification of RNA regulates human development, health, and disease. The true diversity of the transcriptome in breast cancer including chemical modification of transcribed RNA (epitranscriptomics) is not well understood due to limitations of technology and bioinformatic analysis.
Belinda J Petri, Carolyn M Klinge
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Resolving m6A epitranscriptome with stoichiometry
Trends in Genetics, 2022A recent study by Hu et al. describes N6-methyladenosine (m6A)-selective allyl chemical labeling and sequencing (m6A-SAC-seq), which allows for quantitative, stoichiometric, and positional analyses of m6A at single-nucleotide resolution across the whole transcriptome level.
Ki-Jun Yoon, Yoon Ki Kim
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Poly(m6A) tails stabilize transcripts
Molecular Cell, 2022Viegas et al. (2022) discover that in Trypanosoma brucei the poly(A) tails of the variant surface glycoprotein (VSG) transcripts are methylated, a mechanism that stabilizes these transcripts and ensures protection against the immune response in mammals.
Guillaume, Lavergne, Jean-Yves, Roignant
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Journal of Hazardous Materials, 2023
As epigenetic modifications, lactylation and N6-methyladenosine (m6A) have attracted wide attention. Arsenite is an environmental pollutant that has been proven to induce idiopathic pulmonary fibrosis (IPF).
Peiwen Wang +9 more
semanticscholar +1 more source
As epigenetic modifications, lactylation and N6-methyladenosine (m6A) have attracted wide attention. Arsenite is an environmental pollutant that has been proven to induce idiopathic pulmonary fibrosis (IPF).
Peiwen Wang +9 more
semanticscholar +1 more source
Nature Chemical Biology, 2019
The N6-methyladenosine modification on RNA affects almost all steps of RNA metabolism. A new approach, using the CRISPR-based technology to modulate m6A level in mRNA, enables direct functional interrogation of site-specific m6A.
Jiangbo Wei, Chuan He
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The N6-methyladenosine modification on RNA affects almost all steps of RNA metabolism. A new approach, using the CRISPR-based technology to modulate m6A level in mRNA, enables direct functional interrogation of site-specific m6A.
Jiangbo Wei, Chuan He
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m6A nuclear condensates support AML
Nature Reviews Molecular Cell Biology, 2021Nuclear condensates of m6A-methylated mRNAs with their ‘reader’ YTHDC1 prevent the degradation of acute myeloid leukaemia-promoting mRNAs by the exosome.
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Relative Quantification of Residue-Specific m6A RNA Methylation Using m6A-RT-QPCR
2021Technological advances in high-throughput sequencing in combination with antibody enrichment and/or induced nucleotide-specific chemical modifications have accelerated the mapping of epitranscriptomic modifications. However, site-specific detection and quantification of m6A are still technically challenging.
Ane, Olazagoitia-Garmendia +1 more
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METTL3-m6A-EGFR-axis drives lenvatinib resistance in hepatocellular carcinoma.
Cancer Letters, 2023Lenvatinib is emerging as the first-line therapeutic option for advanced hepatocellular carcinoma (HCC), but drug resistance remains a major hurdle for its long-term therapy efficiency in clinic.
Lina Wang +14 more
semanticscholar +1 more source