Results 271 to 280 of about 40,511 (310)

Resolving m6A epitranscriptome with stoichiometry

Trends in Genetics, 2022
A recent study by Hu et al. describes N6-methyladenosine (m6A)-selective allyl chemical labeling and sequencing (m6A-SAC-seq), which allows for quantitative, stoichiometric, and positional analyses of m6A at single-nucleotide resolution across the whole transcriptome level.
Ki-Jun Yoon, Yoon Ki Kim
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Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq

Nature, 2012
An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. Although biochemical studies indicate that N(6)-methyladenosine (m(6)A) is the most prevalent internal modification in messenger RNA, an in-depth study of its distribution and ...
Dan, Dominissini, Sharon, Moshitch-Moshkovitz, Schraga, Schwartz, Mali, Salmon-Divon, Lior, Ungar, Sivan, Osenberg, Karen, Cesarkas, Jasmine, Jacob-Hirsch, Ninette, Amariglio, Martin, Kupiec, Rotem, Sorek, Gideon, Rechavi   +11 more
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An update on principles of m6A targeting

Trends in Plant Science, 2022
N6-methyladenosine (m6A) is of fundamental importance in gene regulation. The function of m6A is achieved through proteins that recognize m6A, known as EVOLUTIONARILY CONSERVED C-TERMINAL REGIONS (ECTs) in arabidopsis (Arabidopsis thaliana). mRNA targets of ECTs and their interaction with m6A-containing motifs remain to be revealed.
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m6A Modification and Implications for microRNAs

MicroRNA, 2017
RNA is chemically modified with over 100 distinct reactions. Among these reactions, methylation is probably the most extensively studied modification on the RNA molecule. Studies suggest methylation of Adenine residues (m6A) to be widespread in the transcriptome with potentially important roles in biological processes.Here, we review recent literature ...
Ayse Elif, Erson-Bensan, Oguzhan, Begik
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Site-specific m6A editing

Nature Chemical Biology, 2019
The N6-methyladenosine modification on RNA affects almost all steps of RNA metabolism. A new approach, using the CRISPR-based technology to modulate m6A level in mRNA, enables direct functional interrogation of site-specific m6A.
Jiangbo Wei, Chuan He
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Role of m6A methylation in retinal diseases

Experimental Eye Research, 2023
Retinal diseases remain among the leading causes of visual impairment in developed countries, despite great efforts in prevention and early intervention. Due to the limited efficacy of current retinal therapies, novel therapeutic methods are urgently required. Over the past two decades, advances in next-generation sequencing technology have facilitated
Xinyu Zhu, Chuandi Zhou, Shuzhi Zhao, Zhi Zheng   +3 more
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Overview of m6A and circRNAs in human cancers

Journal of Cancer Research and Clinical Oncology, 2023
N6-methyladenosine (m6A), the richest post-transcriptional modification of RNA in eukaryotic cells, is dynamically installed/uninstalled by the RNA methylase complex ("writer") and demethylase ("eraser") and recognized by the m6A-binding protein ("reader"). M6A modification on RNA metabolism involves maturation, nuclear export, translation and splicing,
Leyu, Zhang, Xi, Wang, Wei, Zhao, Jingwen, Liu   +3 more
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The m6A methyltransferase METTL3 promotes osteosarcoma progression by regulating the m6A level of LEF1

Biochemical and Biophysical Research Communications, 2019
Osteosarcoma(OS) is the most common and aggressive malignant bone sarcoma,which occurs in rapidly growing bones in children and adolescents. However, the underlying molecular mechanisms of OS development have not been fully illustrated. N6-Methyladenosine (m6A) is the most prevalent internal chemical modification of mRNAs, which is involved in many ...
Wujun, Miao, Jiajia, Chen, Lianshun, Jia, Jun, Ma, Dianwen, Song   +4 more
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Exploring the role of m6A modification in cancer

PROTEOMICS, 2022
Abstract m6A is one of the most common, abundant, and conserved post‐transcriptional modifications that regulate broad biological processes in the human body through m6A regulators. m6A regulators are figuratively classified according to their functions: writers, erasers, and readers, which can methylate RNAs, demethylate RNAs, and ...
Yongqi Wei, Yin Li, Chunlai Lu
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Relative Quantification of Residue-Specific m6A RNA Methylation Using m6A-RT-QPCR

2021
Technological advances in high-throughput sequencing in combination with antibody enrichment and/or induced nucleotide-specific chemical modifications have accelerated the mapping of epitranscriptomic modifications. However, site-specific detection and quantification of m6A are still technically challenging.
Ane, Olazagoitia-Garmendia, Ainara, Castellanos-Rubio   +1 more
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