Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A systematic study on the dynamic recrystallization behavior of S55C during hot deformation
Journal of Materials Research and TechnologyThe hot deformation behavior of S55C steel was systematically investigated via isothermal compression tests over a temperature range of 850–1000 °C and a strain rate range of 0.01–10 s−1.
Wenshuai Yan +5 more
doaj +1 more source
Classification of drilling surface roughness on computer numerical control (CNC) machine tools based on Mobilenet_v3_small_improved [PDF]
Mechanical SciencesComputer numerical control (CNC) machine tool drilling is a crucial process in the contemporary manufacturing sector, facilitating high-precision fabrication of complex components and thus enhancing production efficiency and product quality.
G. Chen +4 more
doaj +1 more source
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
FEBS Open Bio, EarlyView.Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
Fractal and FractionalContact interface is essential for the dynamic response of the bolted structures. To accurately predict the dynamic characteristics of bolted joint structures, a fractal extension of the segmented scale model, i.e., the JK model, is proposed in this ...
Tao Zhang +3 more
doaj +1 more source
Lathe attachment used to machine elliptical cones [PDF]
, 1965 Close-tolerance elliptical cones are fabricated by cutting-tool guide assembly used with conventional tracer cartridge on turret lathe accurately produced in two machine ...
Allen, J. H., Sr., Wobig, O. A.
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Вільна система ЧПУ для верстата паралельної структури [PDF]
, 2013 Application of LinuxCNC (open-source Linux based CNC software) to control parallel kinematic machine tools is considered. A control architecture and configuration files developed for LinuxCNC parallel kinematics machine tool with Stewart platform ...
Kyrychenko, A. +1 more
core
Evaluation of servo, geometric and dynamic error sources on five axis high-speed machine tool
, 2011 Many sources of errors exist in the manufacturing process of complex shapes. Some approximations occur at each step from the design geometry to the machined part.
Andolfatto, Loïc +2 more
core +2 more sources
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source