Results 81 to 90 of about 12,778 (269)

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Improvement of machining accuracy under constant feed speed at milling point with a five axis controlled machining center based on Advanced control Method

Nihon Kikai Gakkai ronbunshu, 2017
Recently, a novel manufacturing technology has spread out with a five-axis machining center, which is controlled by three linear axes and two rotary axes, because the synchronous control technologies make it feasible agilely to create a three-dimensional
Takamaru SUZUKI, Yuma MARUYAMA, Toshiki HIROGAKI, Eiichi AOYAMA   +3 more
doaj   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Reconstruction of Misalignment Aberrations for Cylindrical Surfaces with Complex Parameters in Pseudo Lateral Shearing Interferometry

Sensors
Cylindrical surfaces with complex parameters (CSCPs) have off-axis and aspheric properties. High-precision measurement of cylindrical surfaces is a key research focus in optical metrology.
Yuxuan Ren, Weizhou Luo, Yang Chen, Le Zhao, Liuqing He, Siqi Zhang, Kuo Hai, Xiaodong Zhang, Zhongming Zang   +8 more
doaj   +1 more source

Basic study on process planning for Turning-Milling Center based on machining feature recognition

Journal of Advanced Mechanical Design, Systems, and Manufacturing, 2014
This research aims to develop an automatic process planning system based on the machining feature recognition in the complex machining for Turning-Milling Center. The previous studies on the machining feature recognition are briefly discussed.
Khusna DWIJAYANTI, Hideki AOYAMA
doaj   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Research of the Method of Chamfering Tooth Crest for Spiral Bevel Gear Using Spherical Cutter

Jixie chuandong, 2017
In order to realize chamfering tooth crest for spiral bevel gear efficiently,the feasibility of chamfering method with spherical cutter and machining center is researched.
Wei Wei, Zhang Lianhong
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source