Results 131 to 140 of about 6,829 (247)
Analytical ultracentrifugation (AUC) is a powerful and versatile tool for studying biomolecular interactions. The purpose of this commentary is to provide foundational knowledge of the technique and its uses through the presentation of two case studies in which AUC provides key data to answer research questions.
Liam S. Turk +5 more
wiley +1 more source
ABSTRACT In this study, we analyzed a unique Nicotiana tabacum BY‐2 line that was gradually adapted to and subsequently maintained in 190 mM NaCl for over 15 years. Years of continuous high salinity shaped a stable “new homeostasis” in BY‐2 suspension cells. Salt‐adapted cells were smaller and formed tighter clusters. Metabolomics revealed constitutive
Anita Rzadkiewicz +8 more
wiley +1 more source
Effects of Macromolecular Crowding on the Structure of a Protein Complex: A Small-Angle Scattering Study of Superoxide Dismutase [PDF]
Ajith Rajapaksha +2 more
openalex +1 more source
Membrane‐Associated Biomolecules for Synthetic Cell Signalling
Membrane‐associated proteins and nucleic acids are transforming synthetic cell communication, unlocking applications in biosensing, therapeutics, and tissue engineering. This review explores how these biomolecules enable complementary strategies for cell tethering, vesicle generation and fusion, signal transmission, and signal transduction.
Chelsea Dack +3 more
wiley +1 more source
A Molecular Sensor Reveals Differences in Macromolecular Crowding between the Cytoplasm and Nucleoplasm [PDF]
C.U. Murade, George T. Shubeita
openalex +1 more source
Kinetic and Spatial Resolution by dSTORM of miniSOG‐Mediated Proximity Labeling in Mammalian Cells
Controlled blue‐light illumination defines the kinetic regimes and nanoscale labeling radius of miniSOG in mammalian cells. dSTORM imaging reveals an illumination‐tunable proximal labeling range (∼70–180 nm), establishing quantitative spatial parameters for miniSOG‐mediated photocatalytic tagging and enabling proximity‐labeling strategies.
Benoît Arnould +4 more
wiley +1 more source
Macromolecular crowding in Rhodobacter sphaeroides
Macromolecular crowding is thought to have an important role in protein dynamics and molecular diffusion in bacteria. However, the dynamic and heterogeneous nature of the cytoplasm makes it challenging to study and test the significance of crowding in live cells. Previous studies have used diffusion coefficient of protein to estimate excluded volume in
openaire +2 more sources
Understanding the Impacts of Molecular and Macromolecular Crowding Agents on Protein-Polymer Complex Coacervates. [PDF]
Biswas S +5 more
europepmc +1 more source
Modeling Macromolecular Crowding through Translational and Rotational Diffusion of Small Molecular Probes [PDF]
Megan Currie +4 more
openalex +1 more source
Electroenzymatic CO2 fixation enables energy‐efficient, highly selective synthesis of complex molecules. Unlocking its full potential requires fundamental understanding of electrode‐coupled reductases and carboxylases. This review critically discusses available enzymes, product scope, and key thermodynamic and kinetic considerations, highlighting ...
Leonardo Castañeda‐Losada +4 more
wiley +1 more source

