Results 221 to 230 of about 16,667 (258)

Macromolecular crowding: Macromolecules friend or foe

Biochimica et Biophysica Acta (BBA) - General Subjects, 2015
Cellular interior is known to be densely crowded due to the presence of soluble and insoluble macromolecules, which altogether occupy ~40% of the total cellular volume. This results in altered biological properties of macromolecules.Macromolecular crowding is observed to have both positive and negative effects on protein folding, structure, stability ...
Shruti, Mittal, Rimpy Kaur, Chowhan, Laishram Rajendrakumar, Singh   +2 more
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Transforming eukaryotic cell culture with macromolecular crowding

Trends in Biochemical Sciences, 2021
In multicellular organisms, the intracellular and extracellular spaces are considerably packed with a diverse range of macromolecular species. Yet, standard eukaryotic cell culture is performed in dilute, and deprived of macromolecules culture media, that barely imitate the density and complex macromolecular composition of tissues.
Raghunath, Michael, Zeugolis, Dimitrios I.   +1 more
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Macromolecular crowding: effects on actin polymerisation

Biophysical Chemistry, 1997
Dextran has been found to enhance the polymerisation of actin. This enhancement increases exponentially with increasing mass concentrations of dextran, in a manner that is consistent with excluded volume theory. Mathematical prediction of experimental results is difficult due to the fact that all participating species, namely F-actin, G-actin and ...
R A, Lindner, G B, Ralston
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Macromolecular crowding and molecular recognition

Journal of Molecular Recognition, 1993
AbstractThe effects of macromolecular crowding upon macromolecular associations in solution, and upon binding of a macromolecular ligand to a surface site, ar ereviewed. It is demonstrated, by means of two examples, that crowding may lead to significant alterations of biochemical or biological recognition processes at the molecular level.
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Macromolecular crowding: obvious but underappreciated

Trends in Biochemical Sciences, 2001
Biological macromolecules evolve and function within intracellular environments that are crowded with other macromolecules. Crowding results in surprisingly large quantitative effects on both the rates and the equilibria of interactions involving macromolecules, but such interactions are commonly studied outside the cell in uncrowded buffers.
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Macromolecular crowding effects on protein dynamics

International Journal of Biological Macromolecules
Macromolecular crowding experiments bridge the gap between in-vivo and in-vitro studies by mimicking some of the cellular complexities like high viscosity and limited space, while still manageable for experiments and analysis. Macromolecular crowding impacts all biological processes and is a focus of contemporary research.
Nilimesh Das, Tanmoy Khan, Bisal Halder, Shreya Ghosh, Pratik Sen   +4 more
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An early look at macromolecular crowding

Biophysical Chemistry, 1995
Interest in the interaction between proteins and polysaccharides in semidilute systems developed in the late 1950's and early 1960's both in the field of matrix biology and in the construction of new separation techniques. The author gives an account of how his work in the 50's on the characterization of a connective tissue polysaccharide, hyaluronan ...
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Activity of Subtilisin Carlsberg in macromolecular crowding

Journal of Photochemistry and Photobiology B: Biology, 2007
Enzymatic activity of a proteolytic enzyme Subtilisin Carlsberg (SC) in anionic sodium dodecyl sulfate (SDS) micellar medium has been explored and found to be retarded compared to that in bulk buffer. Circular dichroism (CD) study reveals that SDS, which is a potential protein denaturant, has an insignificant denaturation effect on SC.
Ajay Kumar, Shaw, Samir Kumar, Pal
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Macromolecular Crowding: Biochemical, Biophysical, and Physiological Consequences

Annual Review of Biophysics and Biomolecular Structure, 1993
CONTENTS PERSPECTIVES AND OVERVIEW 28 Nomenclature 30 THEORETICAL CONCEPTS 31 The Effect of Macromolecular C owdin9 on Solution Equilibria 31 The Effect of Macromolecular C owdin# on Reaction Rates 36 The Effect of Macromolecular Crowding on Diffusive Transport of Solutes 37 The Effective Specific Volume of a Macromolecule--What Is It ...
S B, Zimmerman, A P, Minton
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Effects of Macromolecular Crowding on Nuclear Size

Experimental Cell Research, 1995
The concentration of macromolecules inside cells is high, and the resultant crowding of cytoplasm can be expected to affect many interactions involving macromolecular assemblies. Here, we have examined the effect of solute size and concentration on nuclear volume in saponin-permeabilized macrophages.
G R, Rosania, J A, Swanson
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