Results 201 to 210 of about 103,280 (234)

An early look at macromolecular crowding

Biophysical Chemistry, 1995
Interest in the interaction between proteins and polysaccharides in semidilute systems developed in the late 1950's and early 1960's both in the field of matrix biology and in the construction of new separation techniques. The author gives an account of how his work in the 50's on the characterization of a connective tissue polysaccharide, hyaluronan ...
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Macromolecular crowding and molecular recognition

Journal of Molecular Recognition, 1993
AbstractThe effects of macromolecular crowding upon macromolecular associations in solution, and upon binding of a macromolecular ligand to a surface site, ar ereviewed. It is demonstrated, by means of two examples, that crowding may lead to significant alterations of biochemical or biological recognition processes at the molecular level.
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Macromolecular crowding effects on protein dynamics

International Journal of Biological Macromolecules
Macromolecular crowding experiments bridge the gap between in-vivo and in-vitro studies by mimicking some of the cellular complexities like high viscosity and limited space, while still manageable for experiments and analysis. Macromolecular crowding impacts all biological processes and is a focus of contemporary research.
Shreya Ghosh, Pratik Sen, Tanmoy Khan
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Macromolecular Crowding in Mitochondria

The chapter reviews the various effects of crowding on mitochondrial structure and function. Data illustrate that, as a rule, the concentration of macromolecules in the mitochondrial matrix and inner membrane is at least as high as in other parts of the cytoplasm, while the intermembrane space is sparse.
Semen V, Nesterov, Vladimir N, Uversky
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Activity of Subtilisin Carlsberg in macromolecular crowding

Journal of Photochemistry and Photobiology B: Biology, 2007
Enzymatic activity of a proteolytic enzyme Subtilisin Carlsberg (SC) in anionic sodium dodecyl sulfate (SDS) micellar medium has been explored and found to be retarded compared to that in bulk buffer. Circular dichroism (CD) study reveals that SDS, which is a potential protein denaturant, has an insignificant denaturation effect on SC.
Ajay Kumar, Shaw, Samir Kumar, Pal
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Macromolecular crowding: effects on actin polymerisation

Biophysical Chemistry, 1997
Dextran has been found to enhance the polymerisation of actin. This enhancement increases exponentially with increasing mass concentrations of dextran, in a manner that is consistent with excluded volume theory. Mathematical prediction of experimental results is difficult due to the fact that all participating species, namely F-actin, G-actin and ...
R A, Lindner, G B, Ralston
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Macromolecular crowding: Macromolecules friend or foe

Biochimica et Biophysica Acta (BBA) - General Subjects, 2015
Cellular interior is known to be densely crowded due to the presence of soluble and insoluble macromolecules, which altogether occupy ~40% of the total cellular volume. This results in altered biological properties of macromolecules.Macromolecular crowding is observed to have both positive and negative effects on protein folding, structure, stability ...
Shruti, Mittal, Rimpy Kaur, Chowhan, Laishram Rajendrakumar, Singh   +2 more
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Implications of macromolecular crowding for protein assembly

Current Opinion in Structural Biology, 2000
Recent studies have led to increased appreciation of the influence of excluded volume in solutions of high total macromolecular content ('macromolecular crowding') upon the various classes of reaction that lead to the assembly of proteins and protein complexes.
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Effects of Macromolecular Crowding on Nuclear Size

Experimental Cell Research, 1995
The concentration of macromolecules inside cells is high, and the resultant crowding of cytoplasm can be expected to affect many interactions involving macromolecular assemblies. Here, we have examined the effect of solute size and concentration on nuclear volume in saponin-permeabilized macrophages.
G R, Rosania, J A, Swanson
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Cytoskeleton, Motile Structures and Macromolecular Crowding

1994
We descrive the effect of macromolecular crowding on the associations of the cytoskeletal and of the motile structures. These effects are due to preferential interactions and have been treated theoretically by many authors1,2,3,4. Occasionally, preferential interactions are taken into account in the study of the biological reactions, their role ...
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