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Electrical properties of Madin-Darby-canine-kidney cells

Pfl�gers Archiv European Journal of Physiology, 1986
In incompletely confluent Madin Darby canine kidney cells continuous measurements of the potential difference across the cell membrane (PD) were made with conventional microelectrodes during rapid changes of extracellular sodium and/or calcium concentration. During control conditions PD averages -50.6 +/- 0.7 mV.
M, Paulmichl, F, Friedrich, F, Lang
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Ultrastructural study of Crytosporidium development in Madin-Darby canine kidney cells

Veterinary Parasitology, 1993
Transmission electron microscope studies have been made into phases of the life cycle of a bovine isolate of Cryptosporidium cultured in vitro on Madin-Darby canine kidney cells. The cytoplasm of parasitized cells was noticeably altered, including marked vacuolization and the appearance of membrane structures close to the developing parasites.
M J, Rosales, C, Mascaro, A, Osuna
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Electrical properties of Madin-Darby canine kidney cells

Pfl�gers Archiv European Journal of Physiology, 1985
To gain some insight into electrogenic transport processes across the plasma membrane of Madin-Darby canine kidney (MDCK)-cells, continuous measurements of the potential difference across the plasma measurements of the potential difference across the plasma membrane (PD) were made during step changes of extracellular ion composition as well as ...
M, Paulmichl, G, Gstraunthaler, F, Lang
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Calcium Entry Stimulated by Swelling of Madin-Darby Canine Kidney Cells

Nephron, 1996
Cell swelling in Madin-Darby canine kidney (MDCK) cells by reduction of extracellular osmolarity (omission of 70 ad 150 mmol/l mannitol, respectively) leads to the activation of anion of channels and Ca2+ sensitive K+ channels. The K+ channel activation leads to an initial transient hyperpolarization of the cell membrane potential (PD) followed by a ...
E, Wöll   +4 more
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Effect of trifluoperazine on renal epitheloid madin‐darby canine kidney cells

Journal of Cellular Physiology, 1991
AbstractFollowing exposure to a number of hormones, the cell membrane in Madin‐Darby Canine Kidney (MDCK) cells is hyperpolarized by increase of intracellular calcium activity. The present study has been performed to elucidate the possible role of calmodulin in the regulation of intracellular calcium activity and cell membrane potential.
M, Paulmichl   +4 more
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Ca2+ Mobilization Evoked by Chloroform in Madin-Darby Canine Kidney Cells

The Journal of Pharmacology and Experimental Therapeutics, 2000
The effect of chloroform on Ca(2+) mobilization in Madin-Darby canine kidney cells was examined by using Fura-2 as a Ca(2+) probe. Chloroform (24-248 mM) concentration dependently increased intracellular Ca(2+) concentration ([Ca(2+)](i)). Ca(2+) removal inhibited the Ca(2+) signals evoked by 93 to 248 mM chloroform by reducing both the initial rise ...
C R, Jan, L W, Chen, M W, Lin
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Ca2+ mobilization induced by ?-hexachlorocyclohexane in Madin Darby canine kidney cells

Drug Development Research, 2000
The effect of the pesticide δ-hexachlorocyclohexane (δ-HCH) were examined on Ca 2+ signaling in Madin Darby canine kidney (MDCK) using fura-2 as a Ca 2+ probe. δ-HCH at concentrations of 5-200 mM increased intracellular free Ca 2+ concentration ([Ca 2+ ] i ) concentration-dependently. The [Ca 2+ ] i increase comprised an immediate rise followed by
Chung‐Ren Jan   +5 more
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AA-861-Induced Ca2+ Mobilization in Madin Darby Canine Kidney Cells

Toxicology and Applied Pharmacology, 1999
The effect of 2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1, 4-benzoquinone (AA-861), a 5-lipoxygenase inhibitor, on Ca(2+) mobilization in Madin Darby canine kidney (MDCK) cells has been examined by fluorimetry using fura-2 as a Ca(2+) indicator. AA-861 at 10-200 microM increased [Ca(2+)](i) concentration dependently.
J K, Huang, C R, Jan
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Effect of chlorhexidine on Madin Darby canine kidney cells.

Molecular toxicology, 1989
Sheets of MDCK cells were severely damaged by the commonly used antiseptic, chlorhexidine. Damage was indicated by changes in the characteristic blister formation normally observed in sheets of these cells growing on the base of culture flasks. Results are given for reduction in blister number with increasing chlorhexidine concentration.
Z, Jabar, M, Dawson
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Lipoxygenase inhibitor-induced apoptosis in Madin–Darby canine kidney cells

International Congress Series, 2002
Abstract Both cyclooxygenase (COX) and lipoxygenase (LOX), as well as their metabolites, are suggested to play a dual role in regulating cell survival and apoptosis in various types of cells. However, their mechanism remains unclear. When Madin–Darby canine kidney (MDCK) cells were treated with 12-O-tetradecanoylphorbol β-acetate (TPA) and ...
Kohji Nishimura   +6 more
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