Results 41 to 50 of about 20,289 (228)

Microlith Formation In Vitro by Madin Darby Canine Kidney (MDCK) Cells [PDF]

International Journal of Urology, 1996
Background: The mechanism of renal stone genesis as well as the location of stone crystal formation in the kidney remains unclear. Possible sites of stone generation are either in the tubular lumen or tubular cell.Methods: We cultured Madin Darby canine kidney (MDCK), LLC‐PK1 and Magen Krebs Niigata‐28 (MKN‐28) cells in DMEM + 10% FBS medium in a well ...
S, Kageyama, Y, Ohtawara, K, Fujita, T, Watanabe, T, Ushiyama, K, Suzuki, Y, Naito, K, Kawabe   +7 more
openaire   +2 more sources

Attenuation of Influenza a Virus into Live Vaccines Through C‐End Degrons

Advanced Science, EarlyView.
Naturally occurring C‐end degrons are exploited to design proteolysis‐targeting live attenuated influenza vaccines. C‐end degron tagging of viral M1 protein promotes proteasome‐dependent degradation, resulting in robust attenuation in host cells while permitting scalable production in engineered cells.
Ping Wang, Le Li, Yunfang Chen, Le Tong, Zhen Li, Rong Yu, Quan Shen, Qikai Wang, Jihuan Hou, Qisi Zhang, Xu Si, Ning Wang, Demin Zhou, Wen‐xia Tian, Longlong Si   +14 more
wiley   +1 more source

Cell membrane disruption stimulates cAMP and Ca2+ signaling to potentiate cell membrane resealing in neighboring cells

Biology Open, 2017
Disruption of cellular plasma membranes is a common event in many animal tissues, and the membranes are usually rapidly resealed. Moreover, repeated membrane disruptions within a single cell reseal faster than the initial wound in a protein kinase A (PKA)
Tatsuru Togo
doaj   +1 more source

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

Development of the evaluation system for barrier functions of engineered epithelial lumens

Regenerative Therapy, 2016
We have investigated the effects of a diameter of engineered epithelial lumen on cellar architectures and a barrier function. For this investigation, we have developed a system to evaluate the barrier function of engineered epithelial lumens. To test the
Kazuya Furusawa, Takeomi Mizutani, Naoki Sasaki   +2 more
doaj   +1 more source

RNF213 Is an Interferon‐Stimulated Gene That Targets Influenza A Virus NP and Activates MDA5 to Restrict Infection

Advanced Science, EarlyView.
RNF213 is characterized as a dual‐functional antiviral effector. It directly mediates the degradation of the influenza A virus nucleoprotein (NP) while simultaneously activating the MDA5‐mediated innate immune signaling pathway. This coordinated response establishes a powerful host defense system against viral infection. ABSTRACT Influenza A virus (IAV)
Haoning Li, Yuying Zhang, Jingjing Wang, Qianqian Zhang, Hailiang Sun, Fanhua Wei   +5 more
wiley   +1 more source

Environmental hyperosmolality regulates phospholipid biosynthesis in the renal epithelial cell line MDCK

Journal of Lipid Research, 2013
Hyperosmolality is a key signal for renal physiology. On the one hand, it contributes to the differentiation of renal medullary structures and to the development of the urinary concentrating mechanism. On the other, it is a stress factor.
Cecilia I. Casali, Karen Weber, Nicolás O. Favale, María C. Fernández Tome   +3 more
doaj   +1 more source

MVP Inhibits Influenza A Virus‐Induced Ferroptosis by Targeting IRF1 and Increasing FSP1 Activity

Advanced Science, EarlyView.
During IAV infection, MVP inhibits IRF1 polyubiquitination, thereby relieving IRF1‐mediated transcriptional inhibition of FSP1. Consequently, this leads to an upregulation of FSP1 expression, thereby reinforcing the inhibition of ferroptosis. In addition, the MVP can promote myristoylation and ubiquitination of FSP1, enabling its membrane localization ...
Yingbo Chen, Paili Lin, Yongfang Xia, Zhiqiang Liu, Zilu Cheng, Qingmei Zhu, Shiqi Wan, Xiaoyu Chen, Haiyan Bao, Renbo Qiao, Gechang Zhong, Ying Zhu, Shi Liu   +12 more
wiley   +1 more source

Cannabinoids and drug–drug pharmacokinetic interactions: Deciphering the risks

British Journal of Clinical Pharmacology, EarlyView.
The relationship between cannabinoids and mental health has become a major focus of scientific inquiry and public discourse. Cannabinoids are diverse chemical compounds from the Cannabis species that have been explored for their therapeutic applications in treating chronic pain, psychiatric and neurological conditions, such as depression, schizophrenia,
Paraskevi Papakyriakopoulou, Georgia Valsami, Georgios Ismailos   +2 more
wiley   +1 more source

Intranasal vaccination with an engineered influenza virus expressing the receptor binding subdomain of botulinum neurotoxin provides protective immunity against botulism and influenza

Frontiers in Immunology, 2015
Influenza virus is a negative, segmented RNA virus without DNA intermediate. This makes it safer as a vaccine delivery vector than most DNA viruses that have potential to integrate their genetic elements into host genomes.
Junwei eLi, Diana eDiaz-Arévalo, Yanping eChen, Mingtao eZeng   +3 more
doaj   +1 more source