Results 71 to 80 of about 195,003 (295)

MALDI-TOF-MS analysis of Ni²⁺ binding to Hpn.

, 2017
A. Spectra obtained with increasing concentrations of Ni2+ (1:0, 1:1, 1:2.5, 1:5, 1:10, 1:20 and 1:40, mol equivalent from top to bottom, respectively) added to apo-Hpn (25 μM) shown in the right panel, and enlarged view of three representative spectra ...
Hidenori Hayashi (2354416), Rahul Mahadev Shelake (3753109), Yuki Ito (29604), Eugene Hayato Morita (3753112), Junya Masumoto (277060)   +4 more
core   +1 more source

Table_1_Performance evaluation and clinical validation of optimized nucleotide MALDI-TOF-MS for mycobacterial identification.docx

, 2022
ObjectiveTo evaluate the performance and validate the diagnostic value of a nucleotide matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) with the analysis process optimized in identification of mycobacterium species ...
Chi Zhu (11292301), Qi Qi (556563), Meihua Qiu (1710367), Yaping Sun (168299), Hang Dong (406485), Ting Mo (4479901), Lifang Sun (125709), Chao Song (379006), Shangzhi Cao (14206760), Libo Zhen (14206763), Qingshan Cai (8553597), Quanquan Zhang (10027425), Huijie Wang (1655302), Baiying Li (14206757)   +13 more
core   +1 more source

Radical‐Mediated In Situ Fluorescence Dye Deposition: A Simple Interfacial Signal Amplification Reaction for Ultrasensitive Immunoassay on Barcode Beads

Advanced Science, EarlyView.
A radical‐mediated in situ fluorescence dye deposition (RIFD) reaction containing merely three elements of beads, dyes, and radicals is discovered for the first time, and its underlying mechanism is elucidated. This simple RIFD enables the interfacial and ultrafast deposition of free dyes onto barcode beads, serving as a powerful signal amplification ...
Jiayu Zhang, Yao Wang, Xinyi Huang, Haiyu Wu, Qiuning Lin, Hongchen Gu, Li Jiang, Linyong Zhu, Hong Xu   +8 more
wiley   +1 more source

High‐Density E. Coli Cultivation Arrays for Combinatorial Drug Testing With Subsequent Lipid Profiling by MALDI‐MS

Advanced Science, EarlyView.
High‐throughput antibiotic cross‐gradient assays on microdroplet arrays. A multichannel printhead together with five pressure regulators forms droplet arrays of up to 20 832 droplets within 25 minutes. Each droplet contains unique concentrations of antibiotics, allowing to moniture a vast spectrum of bacterial repsonses over night. The bacterial growth
M. Breitfeld, C. L. Dietsche, F‐L. Born, I. Onipko, L. Blaase, P. S. Dittrich   +5 more
wiley   +1 more source

MALDI-TOF MS Analysis of Native and Permethylated or Benzimidazole-Derivatized Polysaccharides

Molecules, 2012
MALDI-TOF MS provides rapid and sensitive analyses of larger biomolecules. However, MS analyses of polysaccharide have been reported to have lower sensitivity compared to peptides and proteins.
Chung-Hsuan Chen, Wen-Bin Yang, Hui-Ming Yu, Yi-Ting Chen, Shwu-Huey Wang, Wei-Ting Hung   +5 more
doaj   +1 more source

Lipidomics of intact mitochondria by MALDI-TOF/MS [PDF]

Journal of Lipid Research, 2012
A simple and fast method of lipid analysis of isolated intact mitochondria by means of MALDI-TOF mass spectrometry is described. Mitochondria isolated from bovine heart and yeast have been employed to set up and validate the new method of lipid analysis.
Angelini, Roberto, Vitale, Rita, Patil, Vinay A., Cocco, Tiziana, Ludwig, Bernd, Greenberg, Miriam L., Corcelli, Angela   +6 more
openaire   +6 more sources

An Aggregation‐Induced Polymerization Poly(Disulfide)‐Drug Nanoplatform for Autoimmune Uveitis Therapy via Inhibiting the cGAS‐STING Pathway

Advanced Science, EarlyView.
A cationic poly(disulfide)‐drug nanoplatform (LA/DexP) was developed to treat experimental autoimmune uveitis (EAU). With potent blood‐retinal barrier penetrability, LA/DexP releases DSP in response to high ROS and scavenges cfDNA to inhibit the cGAS‐STING signaling pathway.
Yuelan Wu, Wenbo Geng, Qinjin Dai, Wanyun Zhang, Yuxian Lai, Pei Zhang, Chunjiang Zhou, Yinuo Wang, Qingfeng Cao, Xiang Luo, Yujie Lai, Changwei Huang, Peizeng Yang   +12 more
wiley   +1 more source

Rapid Multiplex Antimicrobial Resistance Profiling and Bacterial Identification by LAP‐MALDI Mass Spectrometry Biotyping

Advanced Science, EarlyView.
Liquid atmospheric pressure matrix‐assisted laser desorption/ionization (LAP‐MALDI) mass spectrometry (MS) provides multiplexed biotyping of microbial cultures using a fast and simple workflow. This is demonstrated for clinically relevant bacteria, resulting in accurate antimicrobial resistance (AMR) detection, speciation by MS/MS sequencing and ...
Lily R. Adair, Shabnam Iyer, Ian M. Jones, Rainer Cramer   +3 more
wiley   +1 more source

De Novo Discovery of Nonstandard Thioisoindole‐Bridged Bicyclic Peptides Targeting Traf2‐ and NCK‐Interacting Kinase

Angewandte Chemie, EarlyView.
An optimized strategy for the ribosomal synthesis of thioisoindole‐bridged bicyclic (TiB) peptides is incorporated into the RaPID platform, enabling high‐throughput discovery from topologically defined TiB libraries. Proof‐of‐concept selection against TNIK identifies nanomolar‐affinity ligands with inhibitory activity, while x‐ray crystallography ...
Yue Zhang, Alexander A. Vinogradov, Keisuke Hamada, Yin Sun, Toru Sengoku, Hiroaki Suga   +5 more
wiley   +2 more sources

MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain

Chemical and Biological Technologies in Agriculture, 2017
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS; MALDI biotyping) has become a standard tool for the accurate, rapid, and economical identification of pathogens in the clinical diagnostics laboratory. The method
David Drissner, Florian M. Freimoser
doaj   +1 more source