Results 31 to 40 of about 72,918 (250)

LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of Phalaenopsis

Horticulturae, 2019
Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies ...
Hasan Mehraj, Md. Meskatul Alam, Sultana Umma Habiba, Hasan Mehbub   +3 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Comparison The Kinetics of The Uptake of Sugars From The Draw Solution [PDF]

Engineering and Technology Journal, 2016
The present work includes studying the kinetics of the adsorptionof two sugars (maltose and glucose)on activated carbon (GAC) and comparison between them. These sugars are used as draw solution in the forward osmosis process in MOD-SET system(manipulated
Jenan A. Al-Najar, Sami Al-Aibi, Adel O. Sharif, Ghazi Derwish   +3 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Growth performance, jejunal morphology, disaccharidase activities, and sugar transporter gene expression in Langde geese as affected by the in ovo injection of maltose plus sucrose

Frontiers in Veterinary Science, 2023
IntroductionThe vigorous metabolic activity of an embryo increases the risk of low energy supply during incubation. The lack of energy during this critical period will lead to the death of an embryo.
Desheng Li, De Xin Dang, De Xin Dang, Han Xu, Haizhu Zhou, Yujie Lou, Xiao Liu, Yan Cui   +7 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Physiological function of the maltose operon regulator, MalR, in Lactococcus lactis

BMC Microbiology, 2002
Background Maltose metabolism is initiated by an ATP-dependent permease system in Lactococcus lactis. The subsequent degradation of intracellular maltose is performed by the concerted action of Pi-dependent maltose phosphorylase and β-phosphoglucomutase.
Rådström Peter, Andersson Ulrika
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Alteration of Carbohydrate Metabolism in Fusarium Infected Wheat Kernels Treated with Fungicides and Its Relation to Baking Technological Parameters and Deoxynivalenol Contamination

Agriculture, 2023
Changes of water-soluble carbohydrate (WSC) content such as fructose, glucose, sucrose, maltose, nystose, raffinose, stachyose and fructan were analyzed in wheat kernels in Fusarium epidemic and non-epidemic seasons.
Katalin Acs, Monika Varga, Andras Szekeres, Andras Salgo, Csaba Lantos, Ferenc Bekes, Janos Pauk, Akos Mesterhazy   +7 more
doaj   +1 more source

Exploring an Alternative to mRNA Vaccine Cold Chain Storage: MRNA‐Lipid Nanoparticle Stability When Dried in a Polymer Matrix

Advanced Functional Materials, EarlyView.
The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett, Shahad K. Alsaiari, John L. Daristotle, Timothy A. Forster, Alicia Lau, Dhruv Varshney, Rania Afaneh, Andrew R. Hanna, Charles Sloane, David Mankus, Haitao Yu, Jiali Zhai, DongSoo Yun, Lisa H. Tostanoski, Abigail Lytton‐Jean, Dan H. Barouch, Charlotte E. Conn, Robert Langer, Ana Jaklenec, Calum J. Drummond   +19 more
wiley   +1 more source