Results 11 to 20 of about 4,480 (203)

Sequence-Based and Functional Analysis for the Discovery of N-Glycan Degrading Glycosidases From the Microbial Metagenome of the Infant Gut. [PDF]

Microbiologyopen
This research offers valuable understanding of how N‐glycans are broken down by the gut microbiota of infants, focusing on glycoside hydrolase families GH2, GH20, and GH18. It reveals that the gut microbiota of breastfed infants has a diverse array of genes coding for these enzymes.
Boscá-Sánchez I, Rodríguez-Díaz J, Yebra MJ.   +2 more
europepmc   +2 more sources

A β-mannanase with a lysozyme-like fold and a novel molecular catalytic mechanism [PDF]

, 2016
The enzymatic cleavage of β-1,4-mannans is achieved by endo-β-1,4-mannanases, enzymes involved in germination of seeds and microbial hemicellulose degradation, and which have increasing industrial and consumer product applications. β- Mannanases occur in
Cuskin, Fiona, Davies, Gideon J, Gilbert, Harry J, Goddard-Borger, Ethan D, Jenkins, Huw, Jin, Yi, John, Alan, Petricevic, Marija, Portela De Souza, Leticia, Raich, Lluís, Rovira, Carme, Williams, Spencer J   +11 more
core   +21 more sources

Mannosidase mechanism: at the intersection of conformation and catalysis [PDF]

Current Opinion in Structural Biology, 2020
Mannosidases are a diverse group of enzymes that are important in the biological processing of mannose-containing polysaccharides and complex glycoconjugates. They are found in 12 of the >160 sequence-based glycosidase families. We discuss evidence that nature has evolved a small set of common mechanisms that unite almost all of these mannosidase ...
Rovira, Carme, Males, Alexandra, Davies, Gideon J, Williams, Spencer J   +3 more
openaire   +4 more sources

Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration. [PDF]

, 2018
Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.
Kailemia, Muchena J, Lebrilla, Carlito B, Li, Qiongyu, McDonald, Karen A, Nandi, Somen, Xiong, Yongao   +5 more
core   +6 more sources

Towards broad spectrum activity-based glycosidase probes: synthesis and evaluation of deoxygenated cyclophellitol aziridines [PDF]

, 2017
Activity-based protein profiling has emerged as a powerful tool for visualizing glycosidases in complex biological samples. Several configurational cyclophellitol isomers have been shown to display high selectivity as probes for glycosidases processing ...
Adriaan J. Minnaard, Atsumi, Bogdan I. Florea, Chi-Lin Kuo, Davies, Davies, Eva J. van Rooden, Evans, Franck, Gideon J. Davies, Gijsbert A. van der Marel, Hansen, Herman S. Overkleeft, Jasper W. van de Sande, Jeroen D. C. Codée, Jianbing Jiang, Jiang, Jiang, Johannes M. F. G. Aerts, Kallemeijn, Kuharev, Larsbrink, Le Carré, Liu, Long, Luzi, Marta Artola, Nagano, Ohtsubo, Sybrin P. Schröder, Thomas J. M. Beenakker, Tomino, Tony, van Weely, Wendy A. Offen, Willems, Willems, Withers, Witte, Wouter W. Kallemeijn   +39 more
core   +10 more sources

MNSs-mediated N-glycan processing is essential for auxin homeostasis in Arabidopsis roots during alkaline response

iScience, 2022
Summary: Early steps in the endoplasmic reticulum (ER) lumen and cis-Golgi comprise trimming of N-glycans by class I α-mannosidases (MNSs) play crucial roles in root growth and stress response. Herein, we found that the root growth inhibition in the mns1
Tianyu Xia, Yujie Zhan, Yangjie Mu, Jianhua Zhang, Weifeng Xu   +4 more
doaj   +1 more source

A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment

iScience, 2022
Summary: Radiation therapy damages tumors and normal tissues, probably in part through the recruitment of immune cells. Endothelial high-mannose N-glycans are, in particular, involved in monocyte-endothelium interactions.
Ségolène Ladaigue, Anne-Charlotte Lefranc, Khadidiatou Balde, Monica Quitoco, Emilie Bacquer, Didier Busso, Guillaume Piton, Jordane Dépagne, Nathalie Déchamps, Nao Yamakawa, Louise Debusschere, Chunxue Han, Fabrice Allain, Valérie Buard, Georges Tarlet, Agnès François, Vincent Paget, Fabien Milliat, Olivier Guipaud   +18 more
doaj   +1 more source

Les polymères de mannose en production animale. 2. Les enzymes de dégradation des mannanes dans l’alimentation des porcs et des volailles

INRAE Productions Animales, 2021
Les enzymes capables de dégrader les mannanes appartiennent à plusieurs familles d’hydrolases qui se distinguent par les sites de coupure sur ces polysaccharides.
Estelle BONNIN, Michel LESSIRE, Nathaële WACRENIER, Fabien ALLEMAN   +3 more
doaj   +1 more source

Fecal Material of Captive Wild Animals as Source of CAZymes With Application Potential. [PDF]

Chembiochem
With the goal to identify a novel resource of enzymes, we tested for the potential of fecal metagenomes of captive wild animals to provide CAZymes. Our data show that CAZymes profiles differed between animals with varying gut physiology and diet, and that metagenomes of captive wild animals can be considered source of diverse CAZymes including rare and
Bechtner J, Hosek J, Schwab C.
europepmc   +2 more sources

Purification and biochemica characterisation of endoplasmic reticulum α 1,2-mannosidase from Sporothrix schenckiil

Memorias do Instituto Oswaldo Cruz, 2010
Alpha 1,2-mannosidases from glycosyl hydrolase family 47 participate in N-glycan biosynthesis. In filamentous fungi and mammalian cells, α1,2-mannosidases are present in the endoplasmic reticulum (ER) and Golgi complex and are required to generate ...
Héctor M Mora-Montes, Claudia I Robledo-Ortiz, Laura C González-Sánchez, Adolfo López-Esparza, Everardo López-Romero, Arturo Flores-Carreón   +5 more
doaj   +1 more source