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Immunophenotyping by Mass Cytometry
2019Mass cytometry is a novel technology similar to flow cytometry in which antibodies are tagged with heavy metal molecules rather than fluorophores and then detected with time-of-flight mass spectrometry. This enables measurement of up to 50 simultaneous parameters with no autofluorescent background and little or no spillover or required compensation ...
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From mass cytometry to cancer prognosis
Nature Biotechnology, 2015Applying social network algorithms to mass cytometry data from single cancer cells leads to more accurate predictions of patient outcomes.
Deborah R, Winter +2 more
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Tantalum Oxide Nanoparticle-Based Mass Tag for Mass Cytometry
Analytical Chemistry, 2020Mass cytometry (MC) is a bioanalytical technique that uses metal-tagged antibodies (Abs) for high-dimensional single-cell immunoassays. Currently, this technology can measure over 40 parameters simultaneously on individual cells using metal-chelating polymer (MCP) based reagents.
Yefeng Zhang +6 more
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Mass Synaptometry: Applying Mass Cytometry to Single Synapse Analysis
2022Synaptic degeneration is one of the earliest and phenotypically most significant features associated with numerous neurodegenerative conditions, including Alzheimer's and Parkinson's diseases. Synaptic changes are also known to be important in neurocognitive disorders such as schizophrenia and autism spectrum disorders.
Chandresh R, Gajera +6 more
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An Iodinated DAPI‐Based Reagent for Mass Cytometry
ChemBioChem, 2020AbstractMultiparametric single‐cell analysis has seen dramatic improvements with the introduction of mass cytometry (MC) and imaging mass cytometry (IMC™). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy‐isotope‐tagged antibody reagents.
Rahul Rana +5 more
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A Practical Guide to Multiplexed Mass Cytometry
2013Recent advances in inductively coupled plasma mass spectrometry (ICP-MS) as applied in mass cytometry, enabled its broad applicability to life science research. Mass cytometry enables the high-dimensional characterization of cellular systems by simultaneously measuring dozens of metal isotope reporter labeled antibodies bound to cell components.
Zivanovic Nevena +2 more
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Mass cytometry analysis of immune cells in the brain
Nature Protocols, 2018Immune cells comprise a diverse and dynamic cell population that is responsible for a broad range of immunological activities. They act in concert with other immune and nonimmune cells via cytokine-mediated communication and direct cell-cell interactions.
Ben Korin, Tania Dubovik, Asya Rolls
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Organotellurium scaffolds for mass cytometry reagent development
Organic & Biomolecular Chemistry, 2015Tellurophenes are promising biocompatible mass tags.
Hanuel, Park +4 more
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Cell Cycle Analysis by Mass Cytometry
2017The regulated progression of cells through the cell cycle during proliferation is a critical factor in tumor progression, anti-neoplastic therapy response, immune system regulation, and developmental biology. While flow cytometric measurement of cell cycle progression is well established, mass cytometry assays allow the cell cycle to be measured along ...
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Harmonized analysis of PBMC by mass cytometry
Mass cytometry permits the high dimensional analysis of cellular systems at single-cell resolution with high throughput in various areas of biomedical research. Here, we provide a state-of-the-art protocol for the analysis of human peripheral blood mononuclear cells (PBMC) by mass cytometry.Axel R, Schulz +3 more
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