Results 51 to 60 of about 1,192,978 (307)

Rapid Pathogen Identification in Aqueous Humor Samples by Combining Fc-MBL@Fe3O4 Enrichment and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Profiling

Microbiology Spectrum, 2022
Prompt clinical diagnosis and antimicrobial therapy are key to managing infective endophthalmitis. The small volume of aqueous humor, low bacterial counts, and empirical medication by physicians make existing diagnostic methods time-consuming and ...
Jun Ren, Menghuan Yu, Wenjin Gao, Chuanfan Ding, Shengjie Li, Shaoning Yu, Wenjun Cao   +6 more
doaj   +1 more source

Characteristics of the Kelch domain containing (KLHDC) subfamily and relationships with diseases

FEBS Letters, EarlyView.
The Kelch protein superfamily includes 63 members, with the KLHDC subfamily having 10 proteins. While their functions are not fully understood, recent advances in KLHDC2's structure and role in protein degradation have highlighted its potential for drug development, especially in PROTAC therapies.
Courtney Pilcher, Paula Armina V. Buco, Jia Q. Truong, Paul A. Ramsland, Monique F. Smeets, Carl R. Walkley, Jessica K. Holien   +6 more
wiley   +1 more source

Defensive functions and potential ecological conflicts of floral stickiness

Scientific Reports, 2022
Stickiness of vegetative tissues has evolved multiple times in different plant families but is rare and understudied in flowers. While stickiness in general is thought to function primarily as a defense against herbivores, it may compromise mutualistic ...
Alexander Chautá, Arvind Kumar, Jesica Mejia, Elena E. Stashenko, André Kessler   +4 more
doaj   +1 more source

Discrimination of prostate cancer cells and non-malignant cells using secondary ion mass spectrometry [PDF]

, 2008
This communication utilises Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) combined with multivariate analysis to obtain spectra from the surfaces of three closely related cell lines allowing their discrimination based upon mass spectral ...
Baker, Matthew James, Brown, Michael D., Clarke, Noel W., Gazi, Ehsan, Lockyer, Nicholas P., Vickerman, John C.   +5 more
core   +1 more source

The apo LETM1 F‐EF‐hand adopts a closed conformation that underlies a multi‐modal sensory role in mitochondria

FEBS Letters, EarlyView.
We present the first solution structure of the Ca2+‐depleted LETM1 F‐EF‐hand through a D676A/N678A Ca2+ binding‐deficient mutant, revealing a closed hydrophobic cleft caused by a unique F1‐helix pivot. The apo LETM1 F‐EF‐hand exhibits regiospecific hot and cold unfolding, sensitivity to physiological pH changes and potential for promiscuous heterotypic
Qi‐Tong Lin, Danielle M. Colussi, Peter B. Stathopulos   +2 more
wiley   +1 more source

A diverse proteome is present and enzymatically active in metabolite extracts

Nature Communications
Metabolite extraction is the critical first-step in metabolomics experiments, where it is generally regarded to inactivate and remove proteins. Here, arising from efforts to improve extraction conditions for polar metabolomics, we discover a proteomic ...
Rachel (Rae) J. House, Molly T. Soper-Hopper, Michael P. Vincent, Abigail E. Ellis, Colt D. Capan, Zachary B. Madaj, Emily Wolfrum, Christine N. Isaguirre, Carlos D. Castello, Amy B. Johnson, Martha L. Escobar Galvis, Kelsey S. Williams, Hyoungjoo Lee, Ryan D. Sheldon   +13 more
doaj   +1 more source

Seasonal Variation in Stable Isotope Ratios of Cow Milk in Vilnius Region, Lithuania

Animals, 2019
Various studies have shown that stable isotope analysis has the potential to verify the geographic origin of foods and drinks. However, stable isotope composition is not always constant in the environment and can even change in the same area.
Andrius Garbaras, Raminta Skipitytė, Justina Šapolaitė, Žilvinas Ežerinskis, Vidmantas Remeikis   +4 more
doaj   +1 more source

Rapid identification of E. coli bacteriophages using mass spectrometry [PDF]

, 2017
Objective: The current increasing interest in the application of mass spectrometry, in particular MALDI-TOF MS, to identification of bacteria and fungi calls for a need to utilise this technology for identification of other infectious agents such as ...
Pantoja Munoz, Leonardo, Ring, Christopher J., Serafim, Vlad, Shah, Ajit J., Shah, H.   +4 more
core   +1 more source

Mass spectrometry and fullerenes [PDF]

Journal of the American Society for Mass Spectrometry, 1992
This article presents, from amass spectrometry perspective, an historical account of research on gas-phase carbon clusters, which has led to the discovery of another form of carbon, fullerenes. In addition, more recent mass spectrometric studies on analysis of fullerene derivatives and synthesis of doped fullerenes are described.
Stephen W. McElvany, Mark M. Ross
openaire   +3 more sources

Insertion of the FeB cofactor in cNORs lacking metal inserting chaperones

FEBS Letters, EarlyView.
Nitric oxide reductase is an enzyme found in the bacterial denitrification pathway. The NOR active site contains a non‐heme iron, often, but not always inserted with the assistance of chaperones. Here, we study the insertion of FeB in the subfamily of cNORs lacking chaperones and found a putative channel, conserved in the family, perhaps enabling the ...
Sofia Appelgren, Pia Ädelroth
wiley   +1 more source