Results 51 to 60 of about 5,492,223 (387)
Purification tags markedly affect self‐aggregation of CPEB3
FEBS Letters, EarlyView.Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito +6 more
wiley +1 more source
Microbiology Spectrum, 2022 Prompt clinical diagnosis and antimicrobial therapy are key to managing infective endophthalmitis. The small volume of aqueous humor, low bacterial counts, and empirical medication by physicians make existing diagnostic methods time-consuming and ...
Jun Ren +6 more
doaj +1 more source
Mass spectrometry and fullerenes [PDF]
Journal of the American Society for Mass Spectrometry, 1992 This article presents, from amass spectrometry perspective, an historical account of research on gas-phase carbon clusters, which has led to the discovery of another form of carbon, fullerenes. In addition, more recent mass spectrometric studies on analysis of fullerene derivatives and synthesis of doped fullerenes are described.
Stephen W. McElvany, Mark M. Ross
openaire +3 more sources
Circulating histones as clinical biomarkers in critically ill conditions
FEBS Letters, EarlyView.Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez +17 more
wiley +1 more source
A diverse proteome is present and enzymatically active in metabolite extracts
Nature CommunicationsMetabolite extraction is the critical first-step in metabolomics experiments, where it is generally regarded to inactivate and remove proteins. Here, arising from efforts to improve extraction conditions for polar metabolomics, we discover a proteomic ...
Rachel (Rae) J. House +13 more
doaj +1 more source
Defensive functions and potential ecological conflicts of floral stickiness
Scientific Reports, 2022 Stickiness of vegetative tissues has evolved multiple times in different plant families but is rare and understudied in flowers. While stickiness in general is thought to function primarily as a defense against herbivores, it may compromise mutualistic ...
Alexander Chautá +4 more
doaj +1 more source
Interferometric Mass Spectrometry
Journal of the American Society for Mass Spectrometry, 2023 Updated to the published version, J. Am. Soc.
openaire +3 more sources
FEBS Letters, EarlyView.Exposure to common noxious agents (1), including allergens, pollutants, and micro‐nanoplastics, can cause epithelial barrier damage (2) in our body's protective linings. This may trigger an immune response to our microbiome (3). The epithelial barrier theory explains how this process can lead to chronic noncommunicable diseases (4) affecting organs ...
Can Zeyneloglu +17 more
wiley +1 more source
BMC ChemistryA condition-controlled divergent trifluoroalkylation strategy for carbazole derivatives has been developed via alkylation with trifluoropyruvate. Employing trifluoroacetic acid (TFA) promoter, FeCl3 catalyst, or AgSbF6 catalyst, a wide scope of mono ...
Peng Chen +4 more
doaj +1 more source
A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
Nature Communications, 2020 Comprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification
Pan Fang +8 more
doaj +1 more source