Results 61 to 70 of about 1,192,978 (307)

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

Turnover and replication analysis by isotope labeling (TRAIL) reveals the influence of tissue context on protein and organelle lifetimes

Molecular Systems Biology, 2023
The lifespans of proteins range from minutes to years within mammalian tissues. Protein lifespan is relevant to organismal aging, as long‐lived proteins accrue damage over time.
John Hasper, Kevin Welle, Jennifer Hryhorenko, Sina Ghaemmaghami, Abigail Buchwalter   +4 more
doaj   +1 more source

Determination of Creatinine in Human Urine with Flow Injection Tandem Mass Spectrometry [PDF]

, 2012
Background/Aims: Excretion of urinary compounds in spot urine is often estimated relative to creatinine. For the growing number of liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays of urine-excreted molecules, a fast and accurate method ...
Koletzko, Berthold, Niesser, Mareile, Peissner, Wolfgang   +2 more
core   +1 more source

Refining the NaV1.7 pharmacophore of a class of venom‐derived peptide inhibitors via a combination of in silico screening and rational engineering

FEBS Letters, EarlyView.
Venom peptides have shown promise in treating pain. Our study uses computer screening to identify a peptide that targets a sodium channel (NaV1.7) linked to chronic pain. We produced the peptide in the laboratory and refined its design, advancing the search for innovative pain therapies.
Gagan Sharma, Jennifer R. Deuis, Xinying Jia, Theo Crawford, Sassan Rahnama, Eivind A. B. Undheim, Irina Vetter, Yanni K. ‐Y. Chin, Mehdi Mobli   +8 more
wiley   +1 more source

A streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics

Nature Communications, 2020
Comprehensive quantitative profiling of intact glycopeptides remains technically challenging. To address this, the authors here develop an integrated quantitative glycoproteomic workflow, including optimized sample preparation, multiplexed quantification
Pan Fang, Yanlong Ji, Ivan Silbern, Carmen Doebele, Momchil Ninov, Christof Lenz, Thomas Oellerich, Kuan-Ting Pan, Henning Urlaub   +8 more
doaj   +1 more source

Innovative Imaging Techniques: A Conceptual Exploration of Multi-Modal Raman Light Sheet Microscopy

Micromachines, 2023
Advances in imaging of microscopic structures are supported and complemented by adaptive visualization tools. These tools enable researchers to precisely capture and analyze complex three-dimensional structures of different kinds such as crystals ...
Steffen Manser, Shaun Keck, Mario Vitacolonna, Felix Wuehler, Ruediger Rudolf, Matthias Raedle   +5 more
doaj   +1 more source

Mass spectrometry for semi-experimental protein structure determination and modeling [PDF]

arXiv, 2020
The structure of proteins is essential for its function. The determination of protein structures is possible by experimental or predicted by computational methods, but also a combination of both approaches is possible. Here, first an overview about experimental structure determination methods with their pros and cons is given. Then we describe how mass
arxiv  

Mass Conservation And Inference of Metabolic Networks from High-throughput Mass Spectrometry Data [PDF]

J Comput Biol, vol. 18 (2) pp. 147-54, 2011, 2010
We present a step towards the metabolome-wide computational inference of cellular metabolic reaction networks from metabolic profiling data, such as mass spectrometry. The reconstruction is based on identification of irreducible statistical interactions among the metabolite activities using the ARACNE reverse-engineering algorithm and on constraining ...
arxiv   +1 more source

Surfaceome: a new era in the discovery of immune evasion mechanisms of circulating tumor cells

Molecular Oncology, EarlyView.
In the era of immunotherapies, many patients either do not respond or eventually develop resistance. We propose to pave the way for proteomic analysis of surface‐expressed proteins called surfaceome, of circulating tumor cells. This approach seeks to identify immune evasion mechanisms and discover potential therapeutic targets. Circulating tumor cells (
Doryan Masmoudi, Jérome Vialaret, Christophe Hirtz, Catherine Alix‐Panabières   +3 more
wiley   +1 more source

A cosmogenic 10Be anomaly during the late Miocene as independent time marker for marine archives

Nature Communications
Cosmogenic nuclide dating relies on the constancy of production and incorporation of radionuclides in geological archives. Anomalous deviations from constancy during the Holocene or Pleistocene are frequently used as global benchmarks to harmonize ...
Dominik Koll, Johannes Lachner, Sabrina Beutner, Sebastian Fichter, Silke Merchel, Georg Rugel, Zuzana Slavkovská, Carlos Vivo-Vilches, Stella Winkler, Anton Wallner   +9 more
doaj   +1 more source