Results 141 to 150 of about 33,185 (190)

Apical membrane permeability of MDCK cells

American Journal of Physiology-Cell Physiology, 1996
The osmotic water permeability (Pf) and permeability to nonelectrolytes were determined for the apical membrane of clonal strain Madin-Darby canine kidney (MDCK) C12 cells cultured as cysts with the apical membrane facing the surrounding medium. Pf and solute permeabilities were calculated from the rate of volume change of cysts by digitizing images ...
R L, Rivers, J A, McAteer, J L, Clendenon, B A, Connors, A P, Evan, J C, Williams   +5 more
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Cytomatrix synthesis in MDCK epithelial cells

Journal of Cellular Physiology, 1990
AbstractDetailed information regarding the synthesis rates of individual protein components is important in understanding the assembly and dynamics of the cytoskeletal matrix of eukaryotic cells. As an approach to this topic, the dual isotope technique of Clark and Zak J. Biol.
J J, Mitchell, R B, Low, J L, Woodcock-Mitchell   +2 more
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Integrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity

Journal of Cell Science, 1994
ABSTRACT Epithelial cells polarize in response to contacts with the extracellular matrix and with neighboring cells. Interac-tions of cells with the extracellular matrix are mediated mainly by the integrin family of receptors. To begin to understand the role of integrins in polarization, we have investigated the expression and ...
Schoenenberger, C. A., Zuk, A., Zinkl, G. M., Kendall, D., Matlin, K. S.   +4 more
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Electrical properties of cultured epithelioid cells (MDCK)

The Journal of Membrane Biology, 1983
This is a study of the intracellular electrical potential, membrane resistance, and capacity of MDCK cells (epithelioid of renal origin) cultured in monolayers on a collagen couch. These monolayers have a transepithelial resistance of 256 +/- 12 (22) ohm cm2 (mean +/- standard error, and number of observations), and the cells have 61.6 +/- 6.3 (92) M ...
E, Stefani, M, Cereijido
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Methods for Establishing Rab Knockout MDCK Cells

2021
The Rab family small GTPases are key regulators of intracellular membrane traffic that are conserved in all eukaryotic cells. Rabs are thought to regulate various steps of membrane traffic, including the budding, transport, tethering, docking, and fusion of vesicles or organelles.
Riko, Kinoshita, Yuta, Homma, Mitsunori, Fukuda   +2 more
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The Apical Membrane Glycocalyx of MDCK Cells

The Journal of Membrane Biology, 2000
The microenvironment near the apical membrane of MDCK cells was studied by quantitation of the fluorescence of wheat germ agglutinin attached to fluorescein (WGA). WGA was shown to bind to sialic acid residues attached to galactose at the alpha-2,3 position in the glycocalyx on the apical membrane.
O N, Kovbasnjuk, K R, Spring
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Snail modulates cell metabolism in MDCK cells

Biochemical and Biophysical Research Communications, 2013
Snail, a repressor of E-cadherin gene transcription, induces epithelial-to-mesenchymal transition and is involved in tumor progression. Snail also mediates resistance to cell death induced by serum depletion. By contrast, we observed that snail-expressing MDCK (MDCK/snail) cells undergo cell death at a higher rate than control (MDCK/neo) cells in low ...
Misako, Haraguchi, Hiroko P, Indo, Yasumasa, Iwasaki, Yoichiro, Iwashita, Tomoko, Fukushige, Hideyuki J, Majima, Kimiko, Izumo, Masahisa, Horiuchi, Takuro, Kanekura, Tatsuhiko, Furukawa, Masayuki, Ozawa   +10 more
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Regulation of Ripply1 expression in MDCK organoids

Biochemical and Biophysical Research Communications, 2015
Epithelial organs are made of a well-polarized monolayer of epithelial cells, and their morphology must be maintained for their proper function. To examine the genes that are specifically expressed in the late stages of cystogenesis and are involved in maintaining the morphology of the mature cysts, we performed a microarray analysis comparing the mRNA
Hisayoshi, Yoshizaki, Yuka, Kuwajima, Hiroshi, Minato, Etsuko, Kiyokawa   +3 more
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Transfection Alters Ion Transport in MDCK Cells

Journal of Membrane Biology, 1996
In the course of an investigation into the effect of Tamm-Horsfall protein (THP) on ion transport, we performed stable transfection of THP into MDCK cells using the SV40 or the cytomegalovirus (CMV) promoter. As controls, we transfected MDCK cells with an "empty" plasmid containing SV40 or CMV promoter but without THP cDNA.
D M, Kaji, J, Bates, J D, Goyzueta, K, Prasadan, H, Yu, S, Kumar   +5 more
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Measuring receptor recycling in polarized MDCK cells

2015
Recycling of proteins such as channels, pumps, and receptors is critical for epithelial cell function. In this chapter we present a method to measure receptor recycling in polarized Madin-Darby canine kidney cells using an iodinated ligand. We describe a technique to iodinate transferrin (Tf), we discuss how (125)I-Tf can be used to label a cohort of ...
Luciana, Gallo, Gerard, Apodaca
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