Results 171 to 180 of about 57,512 (227)

Comparison between MDCK and MDCK-SIAT1 cell lines as preferred host for cell culture-based influenza vaccine production

Biotechnology Letters, 2016
To evaluate MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus in different Multiplicities of Infection.Yields obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same as MDCK; however, H3N2 virus grown in MDCK-SIAT1 had lower viral titers in comparison with MDCK cells.
Asghar Abdoli   +11 more
openaire   +4 more sources

Quantitative proteomic analysis of MDCK cell adhesion

Molecular Omics, 2021
Establishing a stable MDCK suspension cell line by genetic engineering has significant potential to aid industrialization of vaccine production. In this study, quantitative proteomics was used to explore adhesion proteins in MDCK cells.
Xuanqing Ye   +9 more
openaire   +2 more sources

Snail modulates cell metabolism in MDCK cells

Biochemical and Biophysical Research Communications, 2013
Snail, a repressor of E-cadherin gene transcription, induces epithelial-to-mesenchymal transition and is involved in tumor progression. Snail also mediates resistance to cell death induced by serum depletion. By contrast, we observed that snail-expressing MDCK (MDCK/snail) cells undergo cell death at a higher rate than control (MDCK/neo) cells in low ...
Misako, Haraguchi   +10 more
openaire   +2 more sources

Hydraulic Properties of MDCK Cell Epithelium

Journal of Membrane Biology, 1996
The water permeability of the apical and basolateral cell membranes and the compliance of the lateral intercellular spaces (LIS) of MDCK monolayers were measured on confluent cultures grown on permeable supports. Cell membrane water permeabilities were determined, using quantitative differential interference light microscopy, from the rate of cell ...
M M, Timbs, K R, Spring
openaire   +2 more sources

Apical membrane permeability of MDCK cells

American Journal of Physiology-Cell Physiology, 1996
The osmotic water permeability (Pf) and permeability to nonelectrolytes were determined for the apical membrane of clonal strain Madin-Darby canine kidney (MDCK) C12 cells cultured as cysts with the apical membrane facing the surrounding medium. Pf and solute permeabilities were calculated from the rate of volume change of cysts by digitizing images ...
R L, Rivers   +5 more
openaire   +2 more sources

Cytomatrix synthesis in MDCK epithelial cells

Journal of Cellular Physiology, 1990
AbstractDetailed information regarding the synthesis rates of individual protein components is important in understanding the assembly and dynamics of the cytoskeletal matrix of eukaryotic cells. As an approach to this topic, the dual isotope technique of Clark and Zak J. Biol.
J J, Mitchell   +2 more
openaire   +2 more sources

Integrin expression and localization in normal MDCK cells and transformed MDCK cells lacking apical polarity

Journal of Cell Science, 1994
ABSTRACT Epithelial cells polarize in response to contacts with the extracellular matrix and with neighboring cells. Interac-tions of cells with the extracellular matrix are mediated mainly by the integrin family of receptors. To begin to understand the role of integrins in polarization, we have investigated the expression and ...
Schoenenberger, C. A.   +4 more
openaire   +2 more sources

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