Results 1 to 10 of about 10,158 (214)
The structure of melittin in membranes
Biophysical Journal, 1986 The conformation of the polypeptide melittin in lipid membranes as determined by Raman spectroscopy is a bent alpha-helix formed by the mainly hydrophobic residues 1-21, and a nonhelical COOH-terminal segment of the hydrophilic residues 22-26. Fluorescence quenching experiments on residue Trp19 reveal that all COOH-termini are located on that side of a
Vogel, H., Jähnig, F.
core +4 more sources
Nanoparticle incorporation of melittin reduces sperm and vaginal epithelium cytotoxicity. [PDF]
PLoS ONE, 2014 Melittin is a cytolytic peptide component of bee venom which rapidly integrates into lipid bilayers and forms pores resulting in osmotic lysis. While the therapeutic utility of free melittin is limited by its cytotoxicity, incorporation of melittin into ...
Andrew P Jallouk +6 more
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Emulsion-Based Intradermal Delivery of Melittin in Rats
Molecules, 2017 Bee venom (BV) has long been used as a traditional medicine. The aim of the present study was to formulate a BV emulsion with good rheological properties for dermal application and investigate the effect of formulation on the permeation of melittin ...
Sang Mi Han, Se Gun Kim, Sok Cheon Pak
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Trifluoroacetic acid as excipient destabilizes melittin causing the selective aggregation of melittin within the centrin-melittin-trifluoroacetic acid complex [PDF]
Structural Dynamics, 2015 Trifluoroacetic acid (TFA) may be the cause of the bottleneck in high resolution structure determination for protein-peptide complexes. Fragment based drug design often involves the use of synthetic peptides which contain TFA (excipient). Our goal was to explore the effects of this excipient on a model complex: centrin-melittin-TFA.
Pastrana Rios, Belinda +2 more
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FcIgG-GE11-Melittin as a novel EGFR targeted peptibody with potent cytotoxic activity against cancer cells [PDF]
Scientific ReportsThe overexpression of epidermal growth factor receptor (EGFR) in various cancer types makes it an attractive target for therapeutic intervention. In this study, we designed a novel peptibody, FcIgG-GE11-Melittin, by fusing Melittin, a cytotoxic peptide ...
Malihe Hallaji +5 more
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Kinetics and mechanism of hemolysis induced by melittin and by a synthetic melittin analogue
Biophysical Journal, 1982 The cytotoxic peptide from honeybee venom, melittin, and a synthetic peptide analogue of it lyse human erythrocytes in a biphasic process. The kinetics of the lysis in 0.30 M sucrose, 0.01 M sodium phosphate, pH 7.30 at 4 degrees C were investigated. Our results show that melittin rapidly binds to the outer surface of the erythrocyte membrane, and the ...
DeGrado, W.F. +4 more
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Interaction of melittin with endotoxic lipid A [PDF]
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992 Several amphipathic and cationic substances are known to bind lipid A, the toxic component of bacterial lipopolysaccharides. In this report, we have characterized, by fluorescence methods, the interaction of melittin, an amphipathic and basic 26-residue polypeptide isolated from bee venom, with lipid A.
David, SA +2 more
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A biomimetic nanoparticle-enabled toxoid vaccine against melittin
International Journal of Nanomedicine, 2018 Tianyi Kang,1 Chenyang Li,1,2 Ting Du,1 Yujiao Wu,1 Yuping Yang,1 Xuan Liu,1 Qianqian Zhang,1 Xiaoping Xu,3 Maling Gou1,4 1State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, People&rsquo ...
Kang T +8 more
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The current landscape of the antimicrobial peptide melittin and its therapeutic potential
Frontiers in ImmunologyMelittin, a main component of bee venom, is a cationic amphiphilic peptide with a linear α-helix structure. It has been reported that melittin can exert pharmacological effects, such as antitumor, antiviral and anti-inflammatory effects in vitro and in ...
Na Xu, Liu Wensen
exaly +3 more sources
Journal of Apicultural Science, 2015 Two enzyme-linked immunosorbent assay (ELISA) - based detection systems: indirect competitive ELISA and biotinylated double antibody sandwich ELISA (DAS-ELISA) were developed to determine the melittin concentration in honeybee (Apis mellifera) venom and ...
Suh Lindsey Y. K. +5 more
doaj +2 more sources