Results 31 to 40 of about 11,509 (217)
Ultrastructural observation on sterilization of melittin [PDF]
Science China Life Sciences, 2011 ABSTACT: The effects of melittin on growth and bacteriostasis of four pathogens were extensively investigated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results indicated that the melittin had a marked bacteriostatic effect on the four pathogenic bacteria. Among these, E.
Wang, GuanLin +4 more
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Kinetics and mechanism of hemolysis induced by melittin and by a synthetic melittin analogue
Biophysical Journal, 1982 The cytotoxic peptide from honeybee venom, melittin, and a synthetic peptide analogue of it lyse human erythrocytes in a biphasic process. The kinetics of the lysis in 0.30 M sucrose, 0.01 M sodium phosphate, pH 7.30 at 4 degrees C were investigated. Our results show that melittin rapidly binds to the outer surface of the erythrocyte membrane, and the ...
DeGrado, W.F. +4 more
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On the Mechanism of Pore Formation by Melittin [PDF]
Journal of Biological Chemistry, 2008 The mechanism of pore formation of lytic peptides, such as melittin from bee venom, is thought to involve binding to the membrane surface, followed by insertion at threshold levels of bound peptide. We show that in membranes composed of zwitterionic lipids, i.e. phosphatidylcholine, melittin not only forms pores but also inhibits pore formation.
van den Bogaart, Geert +3 more
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Improved nonviral gene vectors: Efficient and non-toxic polyplexes with enhanced endosomolytic activity [PDF]
, 2005 For the development of improved polyethylenimine (PEI) polyplexes towards ‘artificial viruses’ two key issues are i) to improve the toxicity profile of the applied vectors and ii) to enhance endosomal release, one of the major barriers to efficient gene ...
Boeckle, Sabine
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Journal of Apicultural Science, 2015 Two enzyme-linked immunosorbent assay (ELISA) - based detection systems: indirect competitive ELISA and biotinylated double antibody sandwich ELISA (DAS-ELISA) were developed to determine the melittin concentration in honeybee (Apis mellifera) venom and ...
Suh Lindsey Y. K. +5 more
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Toxins, 2020 Asian honeybee venom is widely used in traditional oriental medicine. Melittin is the main component of Asian honeybee venom. In the present study, an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QqTOF-MS ...
Sheng Huang +4 more
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Structural Dynamics, 2015 Trifluoroacetic acid (TFA) may be the cause of the bottleneck in high resolution structure determination for protein-peptide complexes. Fragment based drug design often involves the use of synthetic peptides which contain TFA (excipient). Our goal was to explore the effects of this excipient on a model complex: centrin-melittin-TFA.
Pastrana Rios, Belinda +2 more
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The Structure of a Melittin-Stabilized Pore [PDF]
Biophysical Journal, 2015 Melittin has been reported to form toroidal pores under certain conditions, but the atomic-resolution structure of these pores is unknown. A 9-μs all-atom molecular-dynamics simulation starting from a closely packed transmembrane melittin tetramer in DMPC shows formation of a toroidal pore after 1 μs.
Leveritt, John M. +2 more
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, 2016 The cytolytic peptide melittin is a potential anticancer candidate that may be able to overcome tumor drug resistance due to its lytic properties. However, in vivo applications of melittin are limited due to its main side effect, hemolysis, which is ...
Honglin Jin (1933081) +6 more
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Structural studies of bee melittin
Biophysical Journal, 1980 The question of how proteins refold in passing from an aqueous phase to an amphipathic environment such as a membrane is beig addressed by a structural study of bee melittin. Melittin is the toxic, main protein of bee venom, and has been shown by others to integrate into natural and synthetic membranes and to lyse a variety of cells.
Eisenberg, David +2 more
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