Results 11 to 20 of about 704,164 (306)

Using flow cytometry for mitochondrial assays

MethodsX, 2020
The understanding of how different cell types adapt their metabolism in the face of challenges has been attracting the attention of researchers for many years. Recently, immunologists also started to focus on how the metabolism of immune cells can impact
Lauar de Brito Monteiro, Gustavo Gastão Davanzo, Cristhiane Favero de Aguiar, Pedro M.M. Moraes-Vieira   +3 more
doaj   +1 more source

Mitochondrial Membrane Potential Assay [PDF]

, 2016
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells.
Srilatha, Sakamuru, Matias S, Attene-Ramos, Menghang, Xia   +2 more
openaire   +2 more sources

Transport of proteins into mitochondria [PDF]

, 1985
Translocational intermediates of precursor proteins of ATPase F1β subunit and cytochrome c1 across mitochondrial membranes were analyzed using two different approaches, transport at low temperature and transport after binding of precursor proteins to ...
Neupert, Walter, Schleyer, Manfred
core   +4 more sources

Biosynthesis of Mitochondrial Porin and Insertion into the Outer Mitochondrial Membrane of Neuruspora crassa [PDF]

, 1982
Mitochondrial porin, the major protein of the outer mitochondrial membrane is synthesized by free cytoplasmic polysomes. The apparent molecular weight of the porin synthesized in homologous or heterologous cell-free systems is the same as that of the ...
Cassady W. E., Colombini M., Conboy J. G., Cote C., Cowlesmith I., Freitag H., Glizin V., Hallermayer R., Hennig B., Korb H., Lewin A., Maccecchini M. L., Maccechini M. L., Matsuura S., Mihara K., Nabi N., Nelson N., Neupert W., Oda T., Palmiter R. D., Payvar F., Raymond Y., Scheele G., Schleyer M., Schmelzer E., Sebald W., Sherton C. C., Shore G. C., Sonderegger P., Teintze M., Ullrich A., Zelman L. S., Zimmermann R., Zimmermann R., Zimmermann R., Zimmermann R.   +35 more
core   +1 more source

Myocardial Imaging for Mitochondrial Membrane Potential

JACC: Cardiovascular Imaging, 2012
Rest and stress myocardial perfusion imaging, described nearly 4 decades ago ([1][1]), is now performed in >9 million people annually in the United States ([2][2]). The procedure provides clinically useful data regardless of whether the tracer is a monovalent cation such as potassium, thallium ...
H William, Strauss, Heiko, Schöder
openaire   +4 more sources

Wafer-scale mitochondrial membrane potential assays [PDF]

Lab on a Chip, 2012
It has been reported that mitochondrial metabolic and biophysical parameters are associated with degenerative diseases and the aging process. To evaluate these biochemical parameters, current technology requires several hundred milligrams of isolated mitochondria for functional assays.
Tae-Sun, Lim, Antonio, Davila, Katayoun, Zand, Douglas C, Wallace, Peter J, Burke   +4 more
openaire   +2 more sources

The mitochondrial Na+/Ca2+ exchanger upregulates glucose dependent Ca2+ signalling linked to insulin secretion. [PDF]

, 2012
Mitochondria mediate dual metabolic and Ca(2+) shuttling activities. While the former is required for Ca(2+) signalling linked to insulin secretion, the role of the latter in β cell function has not been well understood, primarily because the molecular ...
Fishman, Daniel, Hershfinkel, Michal, Khananshvili, Daniel, Lewis, Eli C, Nita, Iulia I, Ozeri, Eyal, Rutter, Guy A, Sekler, Israel, Sensi, Stefano L   +8 more
core   +5 more sources

In-vivo Imaging of Mitochondrial Depolarization of Myocardium With Positron Emission Tomography and a Proton Gradient Uncoupler

Frontiers in Physiology, 2020
BackgroundWe recently reported a method using positron emission tomography (PET) and the tracer 18F-labeled tetraphenylphosphonium (18F-TPP+) for mapping the tissue (i.e., cellular plus mitochondrial) membrane potential (ΔΨT) in the myocardium.
Nathaniel M. Alpert, Matthieu Pelletier-Galarneau, Matthieu Pelletier-Galarneau, Sally Ji Who Kim, Yoann Petibon, Tao Sun, Karla M. Ramos-Torres, Marc D. Normandin, Georges El Fakhri   +8 more
doaj   +1 more source

In silico simulation of reversible and irreversible swelling of mitochondria: The role of membrane rigidity [PDF]

, 2020
Mitochondria have been widely accepted as the main source of ATP in the cell. The inner mitochondrial membrane (IMM) is important for the maintenance of ATP production and other functions of mitochondria.
Arieli, Baranov, Bazil, Bazil, Beavis, Bernardi, Bernardi, Bernardi, Brenner, Carraro, Carroll, Chapa-Dubocq, Crockett, Giorgi, Griffiths, Halestrap, Halestrap, Halestrap, Halestrap, Hollander, Holmuhamedov, Hoppe, Ichas, Ichas, Igor Khmelinskii, Javadov, Javadov, Javadov, Kirichok, Kuznetsov, Kwong, Ladbrooke, Lehninger, Lopez-Crisosto, Makarov, Massari, O'Rourke, Palmer, Petronilli, Pokhilko, Sabzali Javadov, Scalettar, Selivanov, Shinitzky, Song, Szabo, Vladimir I. Makarov, Wodtke, Zaza Khuchua   +48 more
core   +1 more source

Mitochondrial Transmembrane Potential (ψm) Assay Using TMRM

Bio-Protocol, 2013
During cellular respiration, nutrients are oxidized to generate energy through a mechanism called oxidative phosphorylation, which occurs in the mitochondria.
Isaline Rowe, Alessandra Boletta
doaj   +1 more source