Results 11 to 20 of about 9,896,950 (388)

A novel lipoprotein nanoparticle system for membrane proteins [PDF]

Nature Methods, 2016
A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with ...
Jens Frauenfeld, Robin Löving, J. Armache, Andreas F P Sonnen, F. Guettou, P. Moberg, Lin Zhu, C. Jegerschöld, A. Flayhan, J. Briggs, H. Garoff, Christian Löw, Yifan Cheng, P. Nordlund   +13 more
semanticscholar   +4 more sources

Development of a membrane-based Gi-CASE biosensor assay for profiling compounds at cannabinoid receptors

Frontiers in Pharmacology, 2023
Introduction: The cannabinoid receptor (CBR) subtypes 1 (CB1R) and 2 (CB2R) are key components of the endocannabinoid system (ECS), playing a central role in the control of peripheral pain, inflammation and the immune response, with further roles in the ...
Morgan Scott-Dennis, Morgan Scott-Dennis, Fikri A. Rafani, Fikri A. Rafani, Yicheng Yi, Yicheng Yi, Themiya Perera, Themiya Perera, Clare R. Harwood, Clare R. Harwood, Wolfgang Guba, Arne C. Rufer, Uwe Grether, Dmitry B. Veprintsev, Dmitry B. Veprintsev, Dmitry B. Veprintsev, David A. Sykes, David A. Sykes, David A. Sykes   +18 more
doaj   +1 more source

Super-Resolution Imaging Approaches for Quantifying F-Actin in Immune Cells

Frontiers in Cell and Developmental Biology, 2021
Immune cells comprise a diverse set of cells that undergo a complex array of biological processes that must be tightly regulated. A key component of cellular machinery that achieves this is the cytoskeleton.
Evelyn Garlick, Evelyn Garlick, Steven G. Thomas, Steven G. Thomas, Dylan M. Owen, Dylan M. Owen   +5 more
doaj   +1 more source

Probing expression of E-selectin using CRISPR-Cas9-mediated tagging with HiBiT in human endothelial cells

iScience, 2023
Summary: E-selectin is expressed on endothelial cells in response to inflammatory cytokines and mediates leukocyte rolling and extravasation. However, studies have been hampered by lack of experimental approaches to monitor expression in real time in ...
Lydia Ogrodzinski, Simon Platt, Joelle Goulding, Cameron Alexander, Tracy D. Farr, Jeanette Woolard, Stephen J. Hill, Laura E. Kilpatrick   +7 more
doaj   +1 more source

In vitro reconstitution of substrate S-acylation by the zDHHC family of protein acyltransferases

Open Biology, 2022
Protein S-acylation, more commonly known as protein palmitoylation, is a biological process defined by the covalent attachment of long chain fatty acids onto cysteine residues of a protein, effectively altering the local hydrophobicity and influencing ...
R. Elliot Murphy, Anirban Banerjee
doaj   +1 more source

Rampant exchange of the structure and function of extramembrane domains between membrane and water soluble proteins. [PDF]

, 2013
Of the membrane proteins of known structure, we found that a remarkable 67% of the water soluble domains are structurally similar to water soluble proteins of known structure. Moreover, 41% of known water soluble protein structures share a domain with an
Bowie, James U, Han, Seong Kyu, Kim, Sanguk, Nam, Hyun-Jun   +3 more
core   +3 more sources

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi, Aebersold, Aivaliotis, Aivaliotis, Alla, Ames, Babu, Bagshaw, Bell, Berlanda-Scorza, Berven, Bierczynska-Krzysik, Bisle, Bjellqvist, Bunai, Bunai, Burré, Buxbaum, Casey, Chevallet, Chevallet, Connolly, Coughenour, Cutillas, Devreese, Eravci, Everberg, Felsted, Ferro, Gatlin, Ghosh, Gyenes, Görg, Hagan, Hahne, Hartinger, Helling, Henningsen, Henzel, Hisabori, Hjelmeland, Huang, Huang, James, Jun, Lasonder, Lescuyer, Liberatori, Lopez, Low, Luche, Lyubimova, Lüddens, MacFarlane, MacFarlane, Matsudaira, McDonough, Miguet, Moebius, Molloy, Molloy, Nandakumar, Natera, Navarre, Ong, Papasotiriou, Pappin, Patton, Pedersen, Peng, Perdew, Phadke, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rais, Rubin, Santoni, Santoni, Schindler, Schirle, Schägger, Siroy, Speers, Sweetnam, Switzer, Tastet, Taylor, Towbin, Veith, Washburn, Werner, Wilkins, Williams, Williams, Williams, Wittig, Witzmann, Wu, Yates, Ying, Zahedi   +103 more
core   +4 more sources

Structure and dynamics of semaglutide- and taspoglutide-bound GLP-1R-Gs complexes

Cell Reports, 2021
Summary: The glucagon-like peptide-1 receptor (GLP-1R) regulates insulin secretion, carbohydrate metabolism, and appetite and is an important target for treatment of type 2 diabetes and obesity. Multiple GLP-1R agonists have entered into clinical trials,
Xin Zhang, Matthew J. Belousoff, Yi-Lynn Liang, Radostin Danev, Patrick M. Sexton, Denise Wootten   +5 more
doaj   +1 more source

Proteolytic ectodomain shedding of membrane proteins in mammals—hardware, concepts, and recent developments

EMBO Journal, 2018
Proteolytic removal of membrane protein ectodomains (ectodomain shedding) is a post‐translational modification that controls levels and function of hundreds of membrane proteins.
S. Lichtenthaler, M. K. Lemberg, Regina Fluhrer   +2 more
semanticscholar   +1 more source

The Bacterial Photosynthetic Reaction Center as a Model for Membrane Proteins [PDF]

, 1989
Membrane proteins participate in many fundamental cellular processes. Until recently, an understanding of the function and properties of membrane proteins was hampered by an absence of structural information at the atomic level.
Allen, J. P., Feher, G., Komiya, H., Rees, D. C., Yeates, T. O.   +4 more
core   +1 more source