Results 31 to 40 of about 1,959,579 (299)

A Non-imaging High Throughput Approach to Chemical Library Screening at the Unmodified Adenosine-A3 Receptor in Living Cells

Frontiers in Pharmacology, 2017
Recent advances in fluorescent ligand technology have enabled the study of G protein-coupled receptors in their native environment without the need for genetic modification such as addition of N-terminal fluorescent or bioluminescent tags.
Maria Augusta Arruda, Maria Augusta Arruda, Maria Augusta Arruda, Leigh A. Stoddart, Leigh A. Stoddart, Karolina Gherbi, Karolina Gherbi, Stephen J. Briddon, Stephen J. Briddon, Barrie Kellam, Stephen J. Hill, Stephen J. Hill   +11 more
doaj   +1 more source

Structural insight into mitochondrial β-barrel outer membrane protein biogenesis

Nature Communications, 2020
The Sorting and Assembly Machinery (SAM) complex folds beta-barrel proteins and inserts them into the mitochondrial outer membrane. Here authors report cryoEM structures of the SAM complex from Myceliophthora thermophila, which reveals a GST-like fold ...
Kathryn A. Diederichs, Xiaodan Ni, Sarah E. Rollauer, Istvan Botos, Xiaofeng Tan, Martin S. King, Edmund R. S. Kunji, Jiansen Jiang, Susan K. Buchanan   +8 more
doaj   +1 more source

Exploring Ligand Binding to Calcitonin Gene-Related Peptide Receptors

Frontiers in Molecular Biosciences, 2021
Class B1 G protein-coupled receptors (GPCRs) are important targets for many diseases, including cancer, diabetes, and heart disease. All the approved drugs for this receptor family are peptides that mimic the endogenous activating hormones.
Giuseppe Deganutti, Silvia Atanasio, Roxana-Maria Rujan, Patrick M. Sexton, Patrick M. Sexton, Denise Wootten, Denise Wootten, Christopher A. Reynolds   +7 more
doaj   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

TLR3 forms a highly organized cluster when bound to a poly(I:C) RNA ligand

Nature Communications, 2022
TLR3 plays a crucial role in anti-viral immune response by recognizing RNAs produced during virus infection. Here, the authors determine a high resolution structure of the activated cluster of TLR3.
Chan Seok Lim, Yoon Ha Jang, Ga Young Lee, Gu Min Han, Hye Jin Jeong, Ji Won Kim, Jie-Oh Lee   +6 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

A novel and selective fluorescent ligand for the study of adenosine A2B receptors

Pharmacology Research & Perspectives
Fluorescent ligands have proved to be powerful tools in the study of G protein‐coupled receptors in living cells. Here we have characterized a new fluorescent ligand PSB603‐BY630 that has high selectivity for the human adenosine A2B receptor (A2BR).
Foteini Patera, Sarah J. Mistry, Nicholas D. Kindon, Eleonora Comeo, Joelle Goulding, Barrie Kellam, Laura E. Kilpatrick, Hester Franks, Stephen J. Hill   +8 more
doaj   +1 more source

CLEC-2 Prevents Accumulation and Retention of Inflammatory Macrophages During Murine Peritonitis

Frontiers in Immunology, 2021
Platelets play a key role in the development, progression and resolution of the inflammatory response during sterile inflammation and infection, although the mechanism is not well understood.
Joshua H. Bourne, Nonantzin Beristain-Covarrubias, Malou Zuidscherwoude, Malou Zuidscherwoude, Joana Campos, Ying Di, Evelyn Garlick, Evelyn Garlick, Martina Colicchia, Lauren V. Terry, Steven G. Thomas, Steven G. Thomas, Alexander Brill, Alexander Brill, Jagadeesh Bayry, Jagadeesh Bayry, Steve P. Watson, Steve P. Watson, Julie Rayes, Julie Rayes   +19 more
doaj   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source