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The ability to properly identify species present in a landscape is foundational to ecology and essential for natural resource management and conservation.
Mary E. McElroy +19 more
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A manager’s guide to using eDNA metabarcoding in marine ecosystems [PDF]
Environmental DNA (eDNA) metabarcoding is a powerful tool that can enhance marine ecosystem/biodiversity monitoring programs. Here we outline five important steps managers and researchers should consider when developing eDNA monitoring program: (1 ...
Zachary Gold +11 more
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Data to support the in-progress paper https://github.com/alexpiper ...
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Minimizing polymerase biases in metabarcoding
AbstractDNA metabarcoding is an increasingly popular method to characterize and quantify biodiversity in environmental samples. Metabarcoding approaches simultaneously amplify a short, variable genomic region, or “barcode,” from a broad taxonomic group via the polymerase chain reaction (PCR), using universal primers that anneal to flanking conserved ...
Nichols, Ruth V. +7 more
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Metabarcoding is rapidly gaining popularity as a means of conducting biodiversity studies. Using DNA barcodes to identify and catalog biodiversity has many advantages, and compares favorably with traditional methods based on morphological examination ...
Mac P. Pierce
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Ichthyoplankton DNA metabarcoding: Challenges and perspectives [PDF]
DNA metabarcoding has been widely used to access and monitor species. However, several challenges remain open for its mainstream application in ecological studies, particularly when dealing with a quantitative approach. In a From the Cover article in this issue of Molecular Ecology, Mariac et al. (2021) report species‐level ichthyoplankton dynamics for
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DNA Metabarcoding of Amazonian Ichthyoplankton Swarms
Les forêts tropicales humides abritent une biodiversité extraordinaire. On pense que le bassin amazonien abrite 30 % de toutes les espèces de poissons de rivière dans le monde. Les informations sur l'écologie, la reproduction et le recrutement de la plupart des espèces font toujours défaut, ce qui entrave la gestion des pêches et le succès des ...
Marie-Eugénie Maggia +10 more
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Communities of zooplankton, a critical portion of aquatic ecosystems, can be adversely affected by contamination resulting from human activities. Understanding the influence of environmental change on zooplankton communities under field-conditions is ...
Jianghua Yang +6 more
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Fine‐scale estimation of trophic interactions is an important subject in the field of ecology. Diet analysis based on fecal DNA metabarcoding has been accepted as a noninvasive, accurate, and time‐ and cost‐effective tool to determine animal diets. Here,
Haruko Ando +5 more
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Increasing evidence for global insect declines is prompting a renewed interest in the survey of whole insect communities. DNA metabarcoding can contribute to assessing diverse insect communities over a range of spatial and temporal scales, but efforts ...
Vanessa A. Mata +6 more
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