Results 31 to 40 of about 119,445 (233)

Presence of neutrophil extracellular traps (NETs) in different types of human urinary tract infections (UTI). A pilot study

Frontiers in Immunology
IntroductionActivated polymorphonuclear neutrophils (PMN) release neutrophil extracellular traps (NETs) composed of a web-like DNA core, concomitant with nuclear histones, granular peptides and enzymes.
Lena Schröder Alvarez, Lena Schröder Alvarez, Iván Conejeros, Gabriel Espinosa, Constanza Salinas-Varas, Benjamin Ott, Markus Weigel, Markus Weigel, Can Imirzalioglu, Can Imirzalioglu, Moritz Fritzenwanker, Moritz Fritzenwanker, Anita C. Windhorst, Torsten Hain, Torsten Hain, Anja Taubert, Carlos Hermosilla, Florian Wagenlehner, Florian Wagenlehner   +18 more
doaj   +1 more source

A Bioinformatician's Guide to Metagenomics [PDF]

Microbiology and Molecular Biology Reviews, 2008
SUMMARYAs random shotgun metagenomic projects proliferate and become the dominant source of publicly available sequence data, procedures for the best practices in their execution and analysis become increasingly important. Based on our experience at the Joint Genome Institute, we describe the chain of decisions accompanying a metagenomic project from ...
Kunin, V., Copeland, A., Lapidus, A., Mavromatis, K., Hugenholtz, P.   +4 more
openaire   +5 more sources

Mining virulence genes using metagenomics.

PLoS ONE, 2011
When a bacterial genome is compared to the metagenome of an environment it inhabits, most genes recruit at high sequence identity. In free-living bacteria (for instance marine bacteria compared against the ocean metagenome) certain genomic regions are ...
Pedro Belda-Ferre, Raúl Cabrera-Rubio, Andrés Moya, Alex Mira   +3 more
doaj   +1 more source

MMINP: A computational framework of microbe-metabolite interactions-based metabolic profiles predictor based on the O2-PLS algorithm

Gut Microbes, 2023
The gut metabolome acts as an intermediary between the gut microbiota and host, and has tremendous diagnostic and therapeutic potential. Several studies have utilized bioinformatic tools to predict metabolites based on the different aspects of the gut ...
Wenli Tang, Huimin Zheng, Shuangbin Xu, Pan Li, Li Zhan, Xiao Luo, Zehan Dai, Qianwen Wang, Guangchuang Yu   +8 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Metagenomic sequencing enables accurate pathogen and antimicrobial susceptibility profiling in complicated UTIs in approximately four hours

Nature Communications
Urinary tract infections (UTIs) affect 405 million people worldwide. Current diagnostics rely on cultures, which can take 2 to 4 days. This study evaluates eleven culture-independent methods for sample preparation from 78 complicated UTI patients ...
Anurag Basavaraj Bellankimath, Sverre Branders, Isabell Kegel, Jawad Ali, Fatemeh Asadi, Truls E. Bjerklund Johansen, Can Imirzalioglu, Torsten Hain, Florian Wagenlehner, Rafi Ahmad   +9 more
doaj   +1 more source

The Metagenomic Telescope

PLoS ONE, 2014
Next generation sequencing technologies led to the discovery of numerous new microbe species in diverse environmental samples. Some of the new species contain genes never encountered before. Some of these genes encode proteins with novel functions, and some of these genes encode proteins that perform some well-known function in a novel way.
Szalkai, Balázs, Scheer, Ildikó, Nagy, Kinga, G. Vértessy, Beáta, Grolmusz, Vince   +4 more
openaire   +4 more sources

A Single‐Cell Transcriptomic Atlas of the Ovine Rumen Microbiome Characterizes Lineage‐Specific Metabolic Shifts Associated with Host Heat Tolerance

Advanced Science, EarlyView.
An optimized single‐cell transcriptomic framework profiles over 60 000 cells to map the ovine rumen microbiome, partitioning the ecosystem into seven cross‐species functional clusters. In heat‐resistant hosts, a lineage‐specific metabolic shift in Anaerovibrio lipolyticus toward a highly glycolytic phenotype contributes to a “nutritional sparing ...
Sanbao Zhang, Qinyang Jiang, Junjie Ma, Jianwei Zou, Fan Wang, Feifei Lv, Yanna Huang, Yongcheng Wang, Ziye Xu   +8 more
wiley   +1 more source

Metagenomic Association Uncovers Host Genotype‐Structured Rhizobacterial Networks and Novel Taxa That Enhance Soybean Salt Tolerance

Advanced Science, EarlyView.
An STI‐guided metagenomic association framework links soybean salt‐tolerance phenotypes with rhizosphere microbiome composition and network organization in natural saline soils. The approach identifies candidate beneficial taxa, including Thalassospira xiamenensis, and provides an efficient strategy for mining stress‐protective microbes from native ...
Yu Luo, Feng‐Li Kang, Qi‐Min Li, Wei‐Cai Yang   +3 more
wiley   +1 more source

A Comprehensive Microbial Gene Catalog of the Human Airway Microbiome Across Anatomical Sites and Geographic Regions

Advanced Science, EarlyView.
An integrated Human Airway Microbiome Gene Catalog (iHAMGC) is constructed from 12,273 metagenomic airway samples, providing a high‐resolution reference of respiratory microbial genes. The catalog enables systematic exploration of taxonomic and functional variation across airway niches and geographic regions, revealing site‐specific and region ...
Qing Zhang, Dashu Li, Bo Liu, Yue Zhang, Min Li, Ruochun Guo, Yawen Ni, Shibin Chen, Biao Ni, Lijie Qiu, Guorui Xing, He Dong, Qiulong Yan, Shenghui Li, Xiaohui Zou, Bin Cao   +15 more
wiley   +1 more source