Results 51 to 60 of about 33,548 (283)

Steps in Metagenomics: Let’s Avoid Garbage in and Garbage Out [PDF]

, 2015
Is metagenomics a revolution or a new fad? Metagenomics is tightly associated with the availability of next-generation sequencing in all its implementations.
Izard, Jacques
core   +1 more source

DRD2 Deficiency Underlies Pituitary Adenoma Dependent on Escherichia coli Translocation from the Gut

Advanced Science, EarlyView.
Dopamine receptor D2 (DRD2) deficiency disrupts epithelial barrier integrity in both the gut and pituitary gland, facilitating translocation of gut‐derived Escherichia coli into pituitary tissue. Intratumoral E. coli are phagocytosed by microglia, triggering GSDMD‐dependent pyroptosis and HMGB1 release, which subsequently activates MAPK signaling to ...
Xian‐jun Su, Li Ma, Xi Xiong, Jun‐hua Meng, Qi Wu, Yu Zhang, Shu‐guang Dong, Yue‐fei Wang, Jin‐hu Wu, Qing‐yuan Zeng, Hong‐feng Zhang, Li‐li Li, Liang Meng, Min Peng, Xiao‐dong Huang, Li‐quan Wu, Xiong Wang   +16 more
wiley   +1 more source

Clinical Analysis of Metagenomic Next-Generation Sequencing Confirmed Chlamydia psittaci Pneumonia: A Case Series and Literature Review

Infection and Drug Resistance, 2021
Xin-Qi Teng,1,* Wen-Cheng Gong,2,* Ting-Ting Qi,1 Guo-Hua Li,1 Qiang Qu,3 Qiong Lu,1 Jian Qu1 1Department of Pharmacy, The Second Xiangya Hospital, Central South University; Institute of Clinical Pharmacy, Central South University, Changsha, People’
Teng XQ, Gong WC, Qi TT, Li GH, Qu Q, Lu Q, Qu J   +6 more
doaj  

Chronic Neurobrucellosis Investigated via Metagenomic Next-Generation Sequencing

Journal of Clinical Medicine Research, 2020
We found that NGS technology has great significance for the diagnosis of brucellosis in non-epidemic area and can rule out other infections. The number of Brucella gene fragments detected by NGS may be affected by clinical resistance to Brucella treatment and Brucella infection. The sensitivity of SAT to detect Brucella may is limited.
Yun Zhang, Xiaodan Shi, Xiaofei Fei, Guodong Feng, Gang Zhao*   +4 more
openaire   +2 more sources

2‐line Ferrihydrite Enhance Microbial Synthesis of Plant Biostimulants in Composted Biosolid by Regulating Phyla Pseudomonadota and Actinomycetota

Advanced Science, EarlyView.
This study explores how iron and manganese oxides transform sewage sludge into plant biostimulants during composting. Non‐targeted identification reveals the main species of plant biostimulants. Metagenomic analysis reveals that 2‐line ferrihydrite specifically enriches microbial genes for biosynthesis, boosting plant‐growth promoters.
Yu Zhang, Ziwei Zhang, Zexu Chen, Boyuan Yang, Siying Cai, Jun Chen, Jianhua Guo, Weijun Zhang   +7 more
wiley   +1 more source

Enhancing the precision diagnosis and treatment of HIV-related coinfections through metagenomic next-generation sequencing [PDF]

Exploration of Medicine
Aim: Because of severe immunosuppression, coinfections continue to be a major cause of morbidity and mortality in HIV-infected patients. As the primary method currently used for diagnosing coinfections in HIV-infected patients, conventional ...
Yun-Meng Yan, Dong Zhang, Juan Du, Yi Gao, Yao Wang, Min-Ya Lu, Jia-Yu Guo, Hui-Ting Su, Xin-Fei Chen, Ying-Chun Xu, Qi-Wen Yang   +10 more
doaj   +1 more source

Next-generation sequencing of dsRNA is greatly improved by treatment with the inexpensive denaturing reagent DMSO. [PDF]

, 2019
dsRNA is the genetic material of important viruses and a key component of RNA interference-based immunity in eukaryotes. Previous studies have noted difficulties in determining the sequence of dsRNA molecules that have affected studies of immune function
Delwart, Eric, Díaz-Muñoz, Samuel L, Wilcox, Alexander H   +2 more
core  

Considerations for performance metrics of metagenomic next generation sequencing analyses [PDF]

, 2020
AbstractEvaluating the performance of metagenomics analyses has proven a challenge, due in part to limited ground-truth standards, broad application space, and numerous evaluation methods and metrics. Application of traditional clinical performance metrics (i.e.
Jason Kralj, Stephanie L. Servetas, Samuel P. Forry, Scott A. Jackson   +3 more
openaire   +1 more source

Ratoon Season Rice Reduces Methane Emissions by Limiting Acetic Acid Transport to the Rhizosphere and Inhibiting Methanogens

Advanced Science, EarlyView.
This study finds that the interaction between ABA‐OsCIPK2‐OsSWEET1A reduces the allocation of methane producing bacteria carbon source (acetic acid) content to the rhizosphere soil of ratoon season rice, thereby reducing methane emissions. Abstract Rice paddies are a major, persistent source of atmospheric methane (CH4), emission rates depend on the ...
Jingnan Zou, Hailong Xu, Bin Qin, Chaojie Lan, Jinying Li, Bianhong Zhang, Huabin Zhang, Chunlin Guo, Hongfei Chen, Zhongming Fang, Quanzhi Zhao, Wenfei Wang, Changxun Fang, Zhixing Zhang, Wenxiong Lin   +14 more
wiley   +1 more source

Gut Microbiota‐Derived Anandamide Mediates the Therapeutic Effects of Urolithin A on Alcohol‐Induced Cognitive and Social Dysfunction via CB1R‐DRD2‐RAP1 Signaling Axis

Advanced Science, EarlyView.
This study reveals that Urolithin A (UA) counteracts alcohol‐induced cognitive and social dysfunction (AICSD) via a gut microbiome‐dependent mechanism. UA‐enriched Bacteroids sartorii and Parabacteroids distasonis elevate anandamide (AEA), which activates the CB1R‐DRD2‐Rap1 signaling cascade to drive synaptic repair and reduce neuroinflammation ...
Hongbo Zhang, Zibin Li, Yao Xiao, Ji Bian, Caian He, Chao Liu, Lan Gong, Lin Han, Zhigang Liu, Min Wang   +9 more
wiley   +1 more source