Results 41 to 50 of about 6,252 (250)

Metagenomic next-generation sequencing for mixed pulmonary infection diagnosis

BMC Pulmonary Medicine, 2019
Background Metagenomic next-generation sequencing (mNGS) is emerging as a promising technique for pathogens detection. However, reports on the application of mNGS in mixed pulmonary infection remain scarce.
Jiahui Wang, Yelei Han, Jing Feng
doaj   +1 more source

Metagenomic next-generation sequencing in the diagnosis of neurocysticercosis: A case report

World Journal of Clinical Cases, 2023
Inflammatory myofibroblastic tumor (IMT) of the biliary tract is rare, and often difficult to diagnose or to distinguish from other tumors due to its atypical clinical presentation and nonspecific radiological features. Histologically, IMTs are (myo)fibroblastic neoplasms with a prominent inflammatory infiltrate.
Xu, Wen-Bin, Fu, Juan-Juan, Yuan, Xiao-Ju, Xian, Qing-Jie, Zhang, Li-Jun, Song, Ping-Ping, You, Zhi-Qing, Wang, Cheng-Tan, Zhao, Qi-Gang, Pang, Feng   +9 more
openaire   +2 more sources

Species identification and profiling of complex microbial communities using shotgun Illumina sequencing of 16S rRNA amplicon sequences. [PDF]

, 2013
The high throughput and cost-effectiveness afforded by short-read sequencing technologies, in principle, enable researchers to perform 16S rRNA profiling of complex microbial communities at unprecedented depth and resolution. Existing Illumina sequencing
Niranjan Nagarajan, Eliza Xin Pei Ho, Andreas Wilm (7951), Hibberd, Martin Lloyd, Louie Low (400408), Nagarajan, Niranjan, Eliza Xin Pei Ho (400407), Ong, Swee Hoe, Christophe Lay (400406), Martin Lloyd Hibberd (259920), Low, Louie, Kukkillaya, Vinutha Uppoor, Andreas Wilm, Martin Lloyd Hibberd, Lay, Christophe, Niranjan Nagarajan (131213), Wilm, Andreas, Ho, Eliza Xin Pei, Vinutha Uppoor Kukkillaya (400405), Swee Hoe Ong, Louie Low, Swee Hoe Ong (400404), Vinutha Uppoor Kukkillaya, Christophe Lay   +23 more
core   +1 more source

Metagenomic Next-Generation Sequencing for the Diagnosis of Neonatal Infectious Diseases

Microbiology Spectrum, 2022
To the best of our knowledge, this is the first report on evaluating the performance of mNGS using cfDNA and RNA from body fluid and blood samples for diagnosing neonatal infections. mNGS of RNA and cfDNA can achieve the unbiased detection and identification of trace pathogens from different kinds of neonatal body fluid and blood samples with a high ...
Lu Chen, Yujuan Zhao, Jiakai Wei, Wendi Huang, Ying Ma, Xuefeng Yang, Yang Liu, Jing Wang, Han Xia, Zheng Lou   +9 more
openaire   +3 more sources

Two Human Cases of Fatal Meningoencephalitis Associated with Potosi and Lone Star Virus Infections, United States, 2020–2023

Emerging Infectious Diseases
We used clinical metagenomic next-generation sequencing of cerebrospinal fluid to investigate bunyavirus infections in 2 immunocompromised patients in the United States who had fatal meningoencephalitis.
Charles Y. Chiu, Raja Rama Godasi, Holly R. Hughes, Venice Servellita, Kafaya Foresythe, Asritha Tubati, Kelsey Zorn, Sukhman Sidhu, Michael R. Wilson, Sai Varun Bethina, Daniel Abenroth, Yee Cheng, Raymond Grams, Camilla Reese, Carlos Isada, Neeharika Thottempudi   +15 more
doaj   +1 more source

Analytical Tools and Databases for Metagenomics in the Next-Generation Sequencing Era [PDF]

Genomics & Informatics, 2013
Metagenomics has become one of the indispensable tools in microbial ecology for the last few decades, and a new revolution in metagenomic studies is now about to begin, with the help of recent advances of sequencing techniques. The massive data production and substantial cost reduction in next-generation sequencing have led to the rapid growth of ...
Mincheol Kim, Ki-Hyun Lee, Seok-Whan Yoon, Bong-Soo Kim, Jongsik Chun, Hana Yi   +5 more
openaire   +3 more sources

Application of metagenomic next-generation sequencing in patients with infective endocarditis

Frontiers in Cellular and Infection Microbiology, 2023
ObjectivesMetagenomic next-generation sequencing (mNGS) technology is helpful for the early diagnosis of infective endocarditis, especially culture-negative infective endocarditis, which may guide clinical treatment. The purpose of this study was to compare the presence of culture-negative infective endocarditis pathogens versus culture-positive ones ...
Shao-Lin Li, Xi Zhao, Jun-Zhong Tao, Zhen-Zhen Yue, Xiao-Yan Zhao   +4 more
openaire   +3 more sources

Metagenomic next-generation sequencing as a diagnostic tool for toxoplasmic encephalitis [PDF]

Annals of Clinical Microbiology and Antimicrobials, 2018
More than 100 different pathogens can cause encephalitis. Testing of all the neurological pathogens by conventional methods can be difficult. Metagenomic next-generation sequencing (NGS) could identify the infectious agents in a target-independent manner. The role of this novel method in clinical diagnostic microbiology still needs to be evaluated.
Zhiliang Hu, Xing Weng, Chunhua Xu, Yang Lin, Cong Cheng, Hongxia Wei, Wei Chen   +6 more
openaire   +3 more sources

Human Pegivirus Encephalitis With Brain Detection and Response to Sofosbuvir Ledipasvir

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Human pegivirus (HPgV‐1) has been associated with severe encephalomyelitis in immunocompromised patients. Its neurological spectrum remains poorly defined. We report a slowly progressive encephalitis in a person living with well‐controlled HIV, characterized by white matter abnormalities and inflammatory cerebrospinal fluid (CSF). HPgV RNA was
Antoine Moulignier, Françoise Heran, Francis Lallemand, Pierre Bourdillon   +3 more
wiley   +1 more source

QTrim : a novel tool for the quality trimming of sequence reads generated using the Roche/454 sequencing platform [PDF]

, 2014
Background Many high throughput sequencing (HTS) approaches, such as the Roche/454 platform, produce sequences in which the quality of the sequence (as measured by a Phred-like quality scores) decreases linearly across a sequence read.
Grace P McCormack, Lubinsky, Baruch, Bansode, Vijay B., Mónica BJ Moinz, McCormack, Grace P., Vijay B Bansode, Travers, Simon A., Simon A Travers, Ram Shrestha, Baruch Lubinsky, Shrestha, Ram, Moinz, Mónica B. J.   +11 more
core   +1 more source