Results 41 to 50 of about 30,985 (257)

Chronic Meningitis Investigated via Metagenomic Next-Generation Sequencing [PDF]

JAMA Neurology, 2018
Identifying infectious causes of subacute or chronic meningitis can be challenging. Enhanced, unbiased diagnostic approaches are needed.To present a case series of patients with diagnostically challenging subacute or chronic meningitis using metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) supported by a statistical framework ...
Wilson, Michael R, O’Donovan, Brian D, Gelfand, Jeffrey M, Sample, Hannah A, Chow, Felicia C, Betjemann, John P, Shah, Maulik P, Richie, Megan B, Gorman, Mark P, Hajj-Ali, Rula A, Calabrese, Leonard H, Zorn, Kelsey C, Chow, Eric D, Greenlee, John E, Blum, Jonathan H, Green, Gary, Khan, Lillian M, Banerji, Debarko, Langelier, Charles, Bryson-Cahn, Chloe, Harrington, Whitney, Lingappa, Jairam R, Shanbhag, Niraj M, Green, Ari J, Brew, Bruce J, Soldatos, Ariane, Strnad, Luke, Doernberg, Sarah B, Jay, Cheryl A, Douglas, Vanja, Josephson, S Andrew, DeRisi, Joseph L   +31 more
openaire   +4 more sources

Canagliflozin Alleviates Diabetic Glomerular Endothelial Injury via Melibiose in a Microbiota‐Dependent Manner

Advanced Science, EarlyView.
Canagliflozin treatment reshapes the gut microbiota in DKD and elevates levels of melibiose, a metabolite derived from Roseburia intestinalis. Melibiose directly binds to and enhances the enzymatic activity of glyoxalase 1, leading to decreased methylglyoxal accumulation.
Wei Zhang, Yi Song, Changkun Li, Yuanyuan Luo, Mingwei Shao, Feng Guo, Fangyi Wei, Xunjie Fan, Wenwen Guo, Fengmei Xu, Yanhong Sang, Dongming Zhang, Yanhong Zhou, Lianwei Wang, Zhiqiang Kang, Yingjun Yang, Chunhua Song, Yanxia Liu, Xiaojun Ma, Jiao Wang, Chong Li, Shengnan Ma, Lin Zhao, Zhi Qin, Guolan Xing, Qiubo Zhao, Jun Li, Shumin Song, Dan Zhao, Ting Huang, Qingzhu Wang, Yanyan Zhao, Guijun Qin   +32 more
wiley   +1 more source

MFPD: A Multiple Fungal Pathogen Detection Pipeline Across Diverse Habitats

Advanced Science, EarlyView.
The MFPD pipeline integrates a comprehensive ITS reference database of fungal pathogens, optimized parameters, and algorithms tailored for both full‐length and subregion sequences that balance accuracy and computational efficiency; it enables high‐throughput, species‐level identification from amplicon sequencing data, supporting large‐scale ...
Yi Shen, Xinrun Yang, Jiabao Yu, Yaozhong Zhang, Tianjie Yang, Yang Gao, Xiaofang Wang, Alexandre Jousset, Waseem Raza, Fang‐Jie Zhao, Qirong Shen, Gaofei Jiang, Zhong Wei, Yangchun Xu   +13 more
wiley   +1 more source

Polychip‐A High‐Throughput Droplet Microfluidics Platform for Interrogating Microbial Interactions

Advanced Science, EarlyView.
Polychip, a fully integrated droplet microfluidics platform, enables high‐throughput, single‐cell resolution screening of polymicrobial interactions. By seamlessly combining six automated microfluidics operations on a single chip, the system accelerates antimicrobial discovery by 11 to 14 times compared to traditional robotic methods.
Jeong Jae Han, Adrian Ryan Guzman, Aifen Zhou, Han Zhang, Rohit Gupte, Haemin Jung, Kaylee Delgado, Sini Skariah, Ali Sultan, Arul Jayaraman, Paul de Figueiredo, Arum Han   +11 more
wiley   +1 more source

Clinical Analysis of Metagenomic Next-Generation Sequencing Confirmed Chlamydia psittaci Pneumonia: A Case Series and Literature Review

Infection and Drug Resistance, 2021
Xin-Qi Teng,1,* Wen-Cheng Gong,2,* Ting-Ting Qi,1 Guo-Hua Li,1 Qiang Qu,3 Qiong Lu,1 Jian Qu1 1Department of Pharmacy, The Second Xiangya Hospital, Central South University; Institute of Clinical Pharmacy, Central South University, Changsha, People’
Teng XQ, Gong WC, Qi TT, Li GH, Qu Q, Lu Q, Qu J   +6 more
doaj  

Enhancing the precision diagnosis and treatment of HIV-related coinfections through metagenomic next-generation sequencing [PDF]

Exploration of Medicine
Aim: Because of severe immunosuppression, coinfections continue to be a major cause of morbidity and mortality in HIV-infected patients. As the primary method currently used for diagnosing coinfections in HIV-infected patients, conventional ...
Yun-Meng Yan, Dong Zhang, Juan Du, Yi Gao, Yao Wang, Min-Ya Lu, Jia-Yu Guo, Hui-Ting Su, Xin-Fei Chen, Ying-Chun Xu, Qi-Wen Yang   +10 more
doaj   +1 more source

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

Considerations for performance metrics of metagenomic next generation sequencing analyses [PDF]

, 2020
Abstract Evaluating the performance of metagenomics analyses has proven a challenge, due in part to limited ground-truth standards, broad application space, and numerous evaluation methods and metrics. Application of traditional clinical performance metrics (i.e. sensitivity, specificity, etc.) using taxonomic classifiers do not fit the
Jason Kralj, Stephanie L. Servetas, Samuel P. Forry, Scott A. Jackson   +3 more
openaire   +1 more source

A Single‐Cell Transcriptomic Atlas of the Ovine Rumen Microbiome Characterizes Lineage‐Specific Metabolic Shifts Associated with Host Heat Tolerance

Advanced Science, EarlyView.
An optimized single‐cell transcriptomic framework profiles over 60 000 cells to map the ovine rumen microbiome, partitioning the ecosystem into seven cross‐species functional clusters. In heat‐resistant hosts, a lineage‐specific metabolic shift in Anaerovibrio lipolyticus toward a highly glycolytic phenotype contributes to a “nutritional sparing ...
Sanbao Zhang, Qinyang Jiang, Junjie Ma, Jianwei Zou, Fan Wang, Feifei Lv, Yanna Huang, Yongcheng Wang, Ziye Xu   +8 more
wiley   +1 more source

Multi‐Component Functionalized Bifidobacterium Longum Hydrogel for Multi‐Target Integrated Therapy of Colitis‐Associated Anxiety and Depression

Advanced Science, EarlyView.
A pH/MMP dual‐responsive Bifidobacterium longum (BL) hydrogel (INPs@BL@Gel) functionalized with baicalin, tyrosine, and inulin is constructed. It enables gastric protection, inflammation‐targeted release, and prolonged intestinal colonization. By remodeling gut microbiota, elevating beneficial neuroactive metabolites (homovanillic acid, short‐chain ...
Shuo Zhang, Yujie Zhang, Jiansheng He, Shunlian Li, Qingyan Ma, Qiao Li, Yudan Zhang, Yiyang Wang, Shaobo Ma, Songyan Jin, Chune Li, Xueyong Xie, Hang Zhang, Junze Deng, Xueqin Song, Daocheng Wu, Xiancang Ma, Feng Zhu   +17 more
wiley   +1 more source