Results 1 to 10 of about 10,121 (208)

mNGS facilitates the accurate diagnosis and antibiotic treatment of suspicious critical CNS infection in real practice: A retrospective study

Open Life Sciences, 2023
Whether metagenomic next-generation sequencing (mNGS) could benefit patients with suspected severe central nervous system (CNS) infection in terms of diagnosis and antibiotic treatment remains unknown.
Feng Li, Chen Jiaxin, Luo Qiuyan, Su Miao, Chen Peisong, Lai Rong, Shen Cunzhou, Zhou Hongyan, Wang Haiyan, Sun Xunsha, Chen Ling, Xia Han, Feng Huiyu   +12 more
exaly   +2 more sources

Clinical metagenomics [PDF]

Nature Reviews Genetics, 2019
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly moving from research to clinical laboratories. This emerging approach is changing
Charles Y Chiu
exaly   +2 more sources

A Case Report of Cutaneous Anthrax Diagnosed by Using a Metagenomic Next-Generation Sequencing (mNGS) Approach

Infection and Drug Resistance, 2023
Yushan Liu,1,2 Gezhi Zheng,1,3 Jing Li,1,3 Nan Yang,1– 4 Juan Li,1,2 Zhengwen Liu,1– 4 Qunying Han,1– 4 Yingren Zhao,1– 4 Fenjing Du,1,3 Yingli He,1,* Taotao Yan1,* 1Department of Infectious Diseases, The First Affiliated Hospital of Xi’an ...
Qunying Han
exaly   +2 more sources

Clinical analysis of severe Chlamydia psittaci pneumonia: Case series study

Open Life Sciences, 2023
The clinical characteristics and diagnosis of ten cases with severe Chlamydia psittaci pneumonia were analyzed. Ten patients had high fever, cough, or diarrhea, and all had a history of contact with birds or poultry.
Zheng Xi, Wu Chonghao, Jiang Bing, Qin Guangmei, Zeng Ming   +4 more
doaj   +1 more source

Unbiased Metagenomic Sequencing for Pediatric Meningitis in Bangladesh Reveals Neuroinvasive Chikungunya Virus Outbreak and Other Unrealized Pathogens. [PDF]

, 2019
The burden of meningitis in low-and-middle-income countries remains significant, but the infectious causes remain largely unknown, impeding institution of evidence-based treatment and prevention decisions.
Crawford, Emily D., de Bourcy, Charles, De Carvalho, Tiago Rodrigues, DeRisi, Joseph L., Dimitrov, Boris, Duggal, Nisha, Egger, Rebecca, Hasanuzzaman, Md, Islam, Maksuda, Juan, Yun-Fang, Kalantar, Katrina, Khan, Lillian M., Langelier, Charles, Li, Lucy M., Malaker, Roly, Mayday, Madeline Y., Rahman, Hafizur, Ramesh, Akshaya, Saha, Samir K., Saha, Senjuti, Sajib, M. S. I., Sheu, Jonathan, Tang, Jennifer, Tato, Cristina M., Wang, James, Wilson, Michael R.   +25 more
core   +2 more sources

Metagenomic-based Surveillance of Pacific Coast tick Dermacentor occidentalis Identifies Two Novel Bunyaviruses and an Emerging Human Ricksettsial Pathogen. [PDF]

, 2017
An increasing number of emerging tick-borne diseases has been reported in the United States since the 1970s. Using metagenomic next generation sequencing, we detected nucleic acid sequences from 2 novel viruses in the family Bunyaviridae and an emerging ...
Bouquet, Jerome, Chiu, Charles Y, Delwart, Eric, Lane, Robert S, Melgar, Michael, Swei, Andrea   +5 more
core   +2 more sources

Metagenomic next-generation sequencing of samples from pediatric febrile illness in Tororo, Uganda. [PDF]

, 2019
Febrile illness is a major burden in African children, and non-malarial causes of fever are uncertain. In this retrospective exploratory study, we used metagenomic next-generation sequencing (mNGS) to evaluate serum, nasopharyngeal, and stool specimens ...
Byaruhanga, Oswald, de Bourcy, Charles, DeRisi, Joseph L, Dimitrov, Boris, Egger, Rebecca, Hsu, Jennifer, Juan, Yun-Fang, Kalantar, Katrina, Kyohere, Mary, Langelier, Charles, Mpimbaza, Arthur, Nakielny, Sara, Ramesh, Akshaya, Rosenthal, Philip J, Ruel, Theodore, Sheu, Jonathan, Wang, James, Wilson, Michael R   +17 more
core   +3 more sources

An improved protocol for small RNA library construction using High Definition adapters [PDF]

, 2015
Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin, Baran, Bushati, Edgar, Git, Hafner, He, Jagadeeswaran, Jayaprakash, Kawano, Mallory, Mortazavi, Munafó, Pease, Prüfer, Raabe, Reddy, Song, Sorefan, Stocks, Sun, Szittya, Szittya, Tian, Vigneault, Viollet, Willenbrock, Zhang, Zhuang   +28 more
core   +1 more source

Psittacosis Pneumonia: Diagnosis, Treatment and Interhuman Transmission

International Journal of General Medicine, 2023
ZiQi Cui,1 Ling Meng2 1Shandong First Medical University & Shandong Academy of Medical Sciences, Taian, 271000, People’s Republic of China; 2Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital, Shandong First ...
Cui Z, Meng L
doaj  

Evaluation of the Diagnostic Performance of mNGS in Detecting Intra-Abdominal Infections of the Emergency Department Patients

Infection and Drug Resistance, 2023
Liang Zheng,1,* Zhoujun Kang,1,* Ru Wang,2 Meng Lv,2 Zhirui Gao,1 Haizhou Xu,1 Meitang Wang1 1Emergency Department, Changhai Hospital Affiliated to Navy Medical University, Shanghai, People’s Republic of China; 2Genoxor Medical Science and ...
Zheng L, Kang Z, Wang R, Lv M, Gao Z, Xu H, Wang M   +6 more
doaj