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Application of metagenomic next-generation sequencing in the diagnosis of infectious diseases
Frontiers in Cellular and Infection Microbiologyexaly
Clinical Metagenomic Next-Generation Sequencing for Pathogen Detection [PDF]
Annual Review of Pathology: Mechanisms of Disease, 2019 Nearly all infectious agents contain DNA or RNA genomes, making sequencing an attractive approach for pathogen detection. The cost of high-throughput or next-generation sequencing has been reduced by several orders of magnitude since its advent in 2004 ...
Wei Gu, Steve Miller, Charles Y Chiu
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Frontiers in Cellular and Infection MicrobiologyBackgroundWhen individuals infected with human immunodeficiency virus (HIV) experience pulmonary infections, they often exhibit severe symptoms and face a grim prognosis.
Luyao Sun +6 more
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Application of metagenomic next-generation sequencing in cutaneous tuberculosis
Frontiers in Cellular and Infection Microbiology, 2022 Tuberculous infection in a skin wound is a rare but well-known condition. This study describes a child infected with tuberculosis after being wounded. Because of swelling and pain in his wrist tissue, he was admitted to the Affiliated Hospital of Jining ...
Min Kong +7 more
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Leishmaniasis Diagnosis via Metagenomic Next-Generation Sequencing [PDF]
Frontiers in Cellular and Infection Microbiology, 2020 Leishmaniasis is a vector-borne disease caused by Leishmania. Although the incidence of leishmaniasis in China is currently low, it has not been completely eradicated.
Hongbin Chen +6 more
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Frontiers in Cellular and Infection MicrobiologyBackgroundMetagenomic next-generation sequencing (mNGS) technology has been widely used to diagnose various infections. Based on the most common pathogen profiles, targeted mNGS (tNGS) using multiplex PCR has been developed to detect pathogens with ...
Yong Liu +5 more
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Application of Metagenomic Next-Generation Sequencing in Mycobacterium tuberculosis Infection
Frontiers in Medicine, 2022 The fight against Mycobacterium tuberculosis (MTB) has been going on for thousands of years, while it still poses a threat to human health. In addition to routine detections, metagenomic next-generation sequencing (mNGS) has begun to show presence as a ...
Yaoguang Li +7 more
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Pathogenic Detection by Metagenomic Next-Generation Sequencing in Osteoarticular Infections [PDF]
Frontiers in Cellular and Infection Microbiology, 2020 Objectives: To evaluate metagenomic next-generation sequencing (mNGS) as a diagnostic tool in detecting pathogens from osteoarticular infection (OAI) samples.Methods: 130 samples of joint fluid, sonicate fluid, and tissue were prospectively collected ...
Zi-da Huang +8 more
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Metagenomic next‐generation sequencing for the diagnosis of Chlamydia psittaci pneumonia
The Clinical Respiratory Journal, 2022 The aim of this study was to investigate the clinical characteristics of Chlamydia psittaci pneumonia and evaluate the diagnostic value of Metagenomic Next‐Generation Sequencing (mNGS).
Hu Li +13 more
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