Results 31 to 40 of about 4,663,034 (296)

RADSeq: next-generation population genetics [PDF]

, 2010
Next-generation sequencing technologies are making a substantial impact on many areas of biology, including the analysis of genetic diversity in populations.
Davey, John L, M. L. Blaxter, Blaxter, Mark W, J. W. Davey   +3 more
core   +1 more source

Preliminary assessment of nanopore-based metagenomic sequencing for the diagnosis of prosthetic joint infection

International Journal of Infectious Diseases, 2020
Objectives: Pathogen identification is crucial for the diagnosis and management of periprosthetic joint infection (PJI). Although culturing methods are the foundation of pathogen detection in PJI, false-negative results often occur.
Chao-xin Wang, Zida Huang, Wei Fang, Zijie Zhang, Xinyu Fang, Wenbo Li, Bin Yang, Luyao Chen, Xiangzhi Liang, Huan Hu, Duozhi Shi, Shela Lee, Wenming Zhang   +12 more
doaj   +1 more source

Anncaliia algerae Microsporidiosis Diagnosed by Metagenomic Next-Generation Sequencing, China

Emerging Infectious Diseases, 2022
We report a case of Anncaliia algerae microsporidia infection in an immunosuppressed kidney transplant recipient in China. Light microscopy and transmission electron microscopy initially failed to identify A.
Chen Liu, Qin Chen, Ping Fu, Yun-Ying Shi   +3 more
doaj   +1 more source

The diagnostic value of metagenomic next⁃generation sequencing in infectious diseases [PDF]

BMC Infectious Diseases, 2020
Abstract Background: Although traditional diagnostic techniques of infection are mature and price favorable at present, most of them are time-consuming and with a low positivity. Metagenomic next⁃generation sequencing (mNGS) was studied widely because of identification and typing of all pathogens not rely on culture and retrieving all DNA ...
Hongxia Duan, Xuan Li, aihong mei, Ping Li, Yang Liu, Xiaofeng Li, Weiwei Li, Shuanshuan Xie, Changhui Wang   +8 more
openaire   +3 more sources

Computational analysis of next generation sequencing data : from transcription start sites in bacteria to human non-coding RNAS [PDF]

, 2014
The advent of next generation sequencing (NGS) technologies has revolutionized the field of molecular biology by providing a wealth of sequence data. “Transcriptomics”, which aims to identify and annotate the complete set of RNA molecules transcribed ...
Jorjani, Hadi
core   +1 more source

Metagenomic Next-Generation Sequencing for the Diagnosis of Neonatal Infectious Diseases

Microbiology Spectrum, 2022
To the best of our knowledge, this is the first report on evaluating the performance of mNGS using cfDNA and RNA from body fluid and blood samples for diagnosing neonatal infections. mNGS of RNA and cfDNA can achieve the unbiased detection and identification of trace pathogens from different kinds of neonatal body fluid and blood samples with a high ...
Lu Chen, Yujuan Zhao, Jiakai Wei, Wendi Huang, Ying Ma, Xuefeng Yang, Yang Liu, Jing Wang, Han Xia, Zheng Lou   +9 more
openaire   +3 more sources

The Role of Metagenomics and Next-Generation Sequencing in Infectious Disease Diagnosis [PDF]

Clinical Chemistry, 2021
Abstract Background Metagenomic next-generation sequencing (mNGS) for pathogen detection is becoming increasingly available as a method to identify pathogens in cases of suspected infection. mNGS analyzes the nucleic acid content of patient samples with high-throughput sequencing technologies to ...
Steve, Miller, Charles, Chiu
openaire   +2 more sources

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]

, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P., Terauchi, Ryohei, Hideo Matsumura, Schroth Gary P, Krüger Detlev H., Kentaro Yoshida, Kahl, Guenter, Kahl, G., Fujibe, Takahiro, Krueger, Detlev H, Takahiro Fujibe, Luo, Shujun, Fujibe, T., Terauchi Ryohei, Eiji Kimura, Barrero, Roberto A, Yoshida Kentaro, Luo, S., Barrero Roberto A, Terauchi Ryohei, Albertyn Zayed, Luo, Shujun, Shujun Luo, Kimura, Eiji, Barrero, R.A., Schroth, Gary P., Terauchi, Ryohei, Detlev H Krüger, Yoshida Kentaro, Albertyn, Z., Ryohei Terauchi, Roberto A Barrero, Yoshida, Kentaro, Kahl Günter, Matsumura, Hideo, Luo Shujun, Krueger Detlev H, Albertyn Zayed, Gary P. Schroth, Kahl Guenter, Matsumura, Hideo, Fujibe Takahiro, Matsumura Hideo, Yoshida, K., Fujibe, Takahiro, Schroth Gary P., Krüger, D.H., Kahl, Günter, Luo Shujun, Krüger, Detlev H., Kimura, Eiji, Gary P Schroth, Albertyn, Zayed, Matsumura Hideo, Barrero Roberto A., Schroth, Gary P, Zayed Albertyn, Kimura, E., Terauchi, R., Kimura Eiji, Detlev H. Krüger, Albertyn, Zayed, Matsumura, H., Yoshida, Kentaro, Fujibe Takahiro, Barrero, Roberto A., Kimura Eiji, Roberto A. Barrero, Günter Kahl   +68 more
core   +1 more source

Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid

BMC Infectious Diseases, 2022
Background The purpose of this study was to evaluate different pretreatment, extraction, amplification, and library generation methods for metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) and to develop an efficient procedure ...
Hanfang Jiang, Zhihao Xing, Xiaorong Liu, Qiang Chai, Zefeng Xin, Chunqing Zhu, Ruihong Lin, Xuwen Deng, Dong Cui, HongDan Gao, Dongli Ma   +10 more
doaj   +1 more source

Chronic Meningitis Investigated via Metagenomic Next-Generation Sequencing [PDF]

JAMA Neurology, 2018
Identifying infectious causes of subacute or chronic meningitis can be challenging. Enhanced, unbiased diagnostic approaches are needed.To present a case series of patients with diagnostically challenging subacute or chronic meningitis using metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) supported by a statistical framework ...
Wilson, Michael R, O’Donovan, Brian D, Gelfand, Jeffrey M, Sample, Hannah A, Chow, Felicia C, Betjemann, John P, Shah, Maulik P, Richie, Megan B, Gorman, Mark P, Hajj-Ali, Rula A, Calabrese, Leonard H, Zorn, Kelsey C, Chow, Eric D, Greenlee, John E, Blum, Jonathan H, Green, Gary, Khan, Lillian M, Banerji, Debarko, Langelier, Charles, Bryson-Cahn, Chloe, Harrington, Whitney, Lingappa, Jairam R, Shanbhag, Niraj M, Green, Ari J, Brew, Bruce J, Soldatos, Ariane, Strnad, Luke, Doernberg, Sarah B, Jay, Cheryl A, Douglas, Vanja, Josephson, S Andrew, DeRisi, Joseph L   +31 more
openaire   +4 more sources