Results 31 to 40 of about 4,937,404 (296)

Additional file 2 of Can metagenomic next-generation sequencing identify the pathogens responsible for culture-negative prosthetic joint infection?

, 2020
Additional file 2.
Chaoxin Wang (8638119), Xinyu Fang (1419388), Wenbo Li (669721), Wenming Zhang (517008), Zida Huang (8638122)   +4 more
core   +1 more source

From cheek swabs to consensus sequences : an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes [PDF]

, 2014
Background: Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA
Matisoo-Smith, Elizabeth A, Soria Hernanz, David F, Schurr, Theodore G., Stanton, Jo-Ann, Martínez-Cruz, Begoña, White, W. T., GaneshPrasad, Arun Kumar, Lacerda, Daniela R., Matisoo Smith, Elizabeth, White, W. Timothy J., Vilar, Miguel G., Platt, Daniel E., Parida, Laxmi, Hernanz, Soria, Martínez Cruz, Begoña, Kaplan, Matthew E., Clarke, Andrew, Schurr, Theodore G, Matisoo-Smith, Lisa, Matisoo-Smith, Elizabeth, Mitchell, R. John, Matthew E Kaplan, Vieira, Pedro Paulo, Jo-Ann L Stanton, Matisoo-Smith, Elizabeth A., Dulik, Matthew C., Zalloua, Pierre A., Kaplan, Matthew, Tyler-Smith, Chris, Kaplan, M., W Timothy J White, Santhakumari, Arun Varatharajan, Haak, Wolfgang, Quintana-Murci, Lluis, Stefan Prost, null null, Comas, David, Ziegle, Janet S., Swamikrishnan, Pandikumar, Kaplan, Matthew E, Wells, R. Spencer, HASH(0x55a67fb2a340), White, W Timothy J, Cooper, Alan, Clarke, Andrew C, Der Sarkissian, Clio S.I., The, Genographic Consortium, Stanton, J., Bertranpetit, Jaume, 1952-, White, W Timothy J., Stanton, Jo-Ann L., Stanton, Jo Ann L., Pitchappan, Ramasamy, Adhikarla, Syama, Santos, Fabrício R., Comas, David, 1969-, Haber, Marc; id_orcid, Soodyall, Himla, Royyuru, Ajay K., Prost, Stefan, Adler, Christina J., Renfrew, Colin, Jin, Li, Clark, Andrew, Elizabeth A Matisoo-Smith, Andrew C Clarke, Matisoo-Smith, E., Li, Shilin, The Genographic Consortium, Balanovsky, Oleg, Balanovska, Elena, Clarke, Andrew C., Prost, S., Merchant, Nirav C., Andrew C Clarke, Owings, Amanda C., Stanton, Jo-Ann L, White, W., Bertranpetit, Jaume, Gaieski, Jill B., Genographic Consortium, Haber, Marc, 1980-, Genographic Consortium,, Clarke, A.   +83 more
core   +1 more source

Preliminary assessment of nanopore-based metagenomic sequencing for the diagnosis of prosthetic joint infection

International Journal of Infectious Diseases, 2020
Objectives: Pathogen identification is crucial for the diagnosis and management of periprosthetic joint infection (PJI). Although culturing methods are the foundation of pathogen detection in PJI, false-negative results often occur.
Chao-xin Wang, Zida Huang, Wei Fang, Zijie Zhang, Xinyu Fang, Wenbo Li, Bin Yang, Luyao Chen, Xiangzhi Liang, Huan Hu, Duozhi Shi, Shela Lee, Wenming Zhang   +12 more
doaj   +1 more source

Diagnosis and Surveillance of Neonatal Infections by Metagenomic Next-Generation Sequencing

Frontiers in Microbiology, 2022
Microbial infections cause significant morbidity and mortality in neonates. Metagenomic next-generation sequencing is a hypothesis-free and culture-free test that enables broad identification of pathogens and antimicrobial resistance genes directly from clinical samples within 24 h.
Rong Zhang, Yan Zhuang, Zheng-hui Xiao, Cai-yun Li, Fan Zhang, Wei-qing Huang, Min Zhang, Xiao-Ming Peng, Chao Liu   +8 more
openaire   +3 more sources

The Role of Metagenomics and Next-Generation Sequencing in Infectious Disease Diagnosis [PDF]

Clinical Chemistry, 2021
Abstract Background Metagenomic next-generation sequencing (mNGS) for pathogen detection is becoming increasingly available as a method to identify pathogens in cases of suspected infection. mNGS analyzes the nucleic acid content of patient samples with high-throughput sequencing technologies to ...
Steve, Miller, Charles, Chiu
openaire   +2 more sources

RADSeq: next-generation population genetics [PDF]

, 2010
Next-generation sequencing technologies are making a substantial impact on many areas of biology, including the analysis of genetic diversity in populations.
Davey, John L, M. L. Blaxter, Blaxter, Mark W, J. W. Davey   +3 more
core   +1 more source

Anncaliia algerae Microsporidiosis Diagnosed by Metagenomic Next-Generation Sequencing, China

Emerging Infectious Diseases, 2022
We report a case of Anncaliia algerae microsporidia infection in an immunosuppressed kidney transplant recipient in China. Light microscopy and transmission electron microscopy initially failed to identify A.
Chen Liu, Qin Chen, Ping Fu, Yun-Ying Shi   +3 more
doaj   +1 more source

Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid

BMC Infectious Diseases, 2022
Background The purpose of this study was to evaluate different pretreatment, extraction, amplification, and library generation methods for metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) and to develop an efficient procedure ...
Hanfang Jiang, Zhihao Xing, Xiaorong Liu, Qiang Chai, Zefeng Xin, Chunqing Zhu, Ruihong Lin, Xuwen Deng, Dong Cui, HongDan Gao, Dongli Ma   +10 more
doaj   +1 more source

A Rare Bird: Diagnosis of Psittacosis Meningitis by Clinical Metagenomic Next-Generation Sequencing [PDF]

, 2021
Psittacosis is a zoonotic infectious disease caused by the transmission of Chlamydia psittaci; it often presents as a pulmonary infection but rarely as disseminated disease.
Chiu, CY, Miller, S, Davar, K, Wilson, MR, Vijayan, T, T Vijayan, M R Wilson, C Y Chiu, S Miller, K Davar   +9 more
core   +1 more source

Assessment of metagenomic assembly using simulated next generation sequencing data. [PDF]

PLoS ONE, 2012
Due to the complexity of the protocols and a limited knowledge of the nature of microbial communities, simulating metagenomic sequences plays an important role in testing the performance of existing tools and data analysis methods with metagenomic data ...
Daniel R Mende, Alison S Waller, Shinichi Sunagawa, Aino I Järvelin, Michelle M Chan, Manimozhiyan Arumugam, Jeroen Raes, Peer Bork   +7 more
doaj   +1 more source