Results 31 to 40 of about 6,252 (250)

The Role of Metagenomics and Next-Generation Sequencing in Infectious Disease Diagnosis [PDF]

Clinical Chemistry, 2021
Abstract Background Metagenomic next-generation sequencing (mNGS) for pathogen detection is becoming increasingly available as a method to identify pathogens in cases of suspected infection. mNGS analyzes the nucleic acid content of patient samples with high-throughput sequencing technologies to ...
Steve, Miller, Charles, Chiu
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Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid

BMC Infectious Diseases, 2022
Background The purpose of this study was to evaluate different pretreatment, extraction, amplification, and library generation methods for metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) and to develop an efficient procedure ...
Hanfang Jiang, Zhihao Xing, Xiaorong Liu, Qiang Chai, Zefeng Xin, Chunqing Zhu, Ruihong Lin, Xuwen Deng, Dong Cui, HongDan Gao, Dongli Ma   +10 more
doaj   +1 more source

Anncaliia algerae Microsporidiosis Diagnosed by Metagenomic Next-Generation Sequencing, China

Emerging Infectious Diseases, 2022
We report a case of Anncaliia algerae microsporidia infection in an immunosuppressed kidney transplant recipient in China. Light microscopy and transmission electron microscopy initially failed to identify A.
Chen Liu, Qin Chen, Ping Fu, Yun-Ying Shi   +3 more
doaj   +1 more source

Computational analysis of next generation sequencing data : from transcription start sites in bacteria to human non-coding RNAS [PDF]

, 2014
The advent of next generation sequencing (NGS) technologies has revolutionized the field of molecular biology by providing a wealth of sequence data. “Transcriptomics”, which aims to identify and annotate the complete set of RNA molecules transcribed ...
Jorjani, Hadi
core   +1 more source

Diagnosis and Surveillance of Neonatal Infections by Metagenomic Next-Generation Sequencing

Frontiers in Microbiology, 2022
Microbial infections cause significant morbidity and mortality in neonates. Metagenomic next-generation sequencing is a hypothesis-free and culture-free test that enables broad identification of pathogens and antimicrobial resistance genes directly from clinical samples within 24 h.
Rong Zhang, Yan Zhuang, Zheng-hui Xiao, Cai-yun Li, Fan Zhang, Wei-qing Huang, Min Zhang, Xiao-Ming Peng, Chao Liu   +8 more
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The Early Diagnosis of Scrub Typhus by Metagenomic Next-Generation Sequencing [PDF]

Frontiers in Public Health, 2021
Introduction: Scrub typhus is a mite-borne infection widespread in Southeast Asia, with clinical symptoms such as fever, chills, skin rash, eschar at the bite site, and other signs of acute febrile illness. The Rickettsia pathogen (Orientia tsutsugamushi) is always difficult to be diagnosed at an early stage by traditional clinical diagnostic methods ...
Xianghong Liu, Ye Zhang, Jun Zhang, Zheng Lou, Han Xia, Zhijuan Lu   +5 more
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Preliminary assessment of nanopore-based metagenomic sequencing for the diagnosis of prosthetic joint infection

International Journal of Infectious Diseases, 2020
Objectives: Pathogen identification is crucial for the diagnosis and management of periprosthetic joint infection (PJI). Although culturing methods are the foundation of pathogen detection in PJI, false-negative results often occur.
Chao-xin Wang, Zida Huang, Wei Fang, Zijie Zhang, Xinyu Fang, Wenbo Li, Bin Yang, Luyao Chen, Xiangzhi Liang, Huan Hu, Duozhi Shi, Shela Lee, Wenming Zhang   +12 more
doaj   +1 more source

Assessment of metagenomic assembly using simulated next generation sequencing data. [PDF]

PLoS ONE, 2012
Due to the complexity of the protocols and a limited knowledge of the nature of microbial communities, simulating metagenomic sequences plays an important role in testing the performance of existing tools and data analysis methods with metagenomic data ...
Daniel R Mende, Alison S Waller, Shinichi Sunagawa, Aino I Järvelin, Michelle M Chan, Manimozhiyan Arumugam, Jeroen Raes, Peer Bork   +7 more
doaj   +1 more source

Metagenomic Next-Generation Sequencing for Diagnostically Challenging Mucormycosis in Patients with Hematological Malignancies

Infection and Drug Resistance, 2022
Meng Zhang,1,* Wenyi Lu,2,* Danni Xie,1 Jiaxi Wang,1 Xia Xiao,2 Yedi Pu,2 Juanxia Meng,2 Hairong Lyu,2 Mingfeng Zhao2 1First Center Clinic College of Tianjin Medical University, Tianjin, 300192, People’s Republic of China; 2Department of ...
Zhang M, Lu W, Xie D, Wang J, Xiao X, Pu Y, Meng J, Lyu H, Zhao M   +8 more
doaj  

Chronic Meningitis Investigated via Metagenomic Next-Generation Sequencing [PDF]

JAMA Neurology, 2018
Identifying infectious causes of subacute or chronic meningitis can be challenging. Enhanced, unbiased diagnostic approaches are needed.To present a case series of patients with diagnostically challenging subacute or chronic meningitis using metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) supported by a statistical framework ...
Wilson, Michael R, O’Donovan, Brian D, Gelfand, Jeffrey M, Sample, Hannah A, Chow, Felicia C, Betjemann, John P, Shah, Maulik P, Richie, Megan B, Gorman, Mark P, Hajj-Ali, Rula A, Calabrese, Leonard H, Zorn, Kelsey C, Chow, Eric D, Greenlee, John E, Blum, Jonathan H, Green, Gary, Khan, Lillian M, Banerji, Debarko, Langelier, Charles, Bryson-Cahn, Chloe, Harrington, Whitney, Lingappa, Jairam R, Shanbhag, Niraj M, Green, Ari J, Brew, Bruce J, Soldatos, Ariane, Strnad, Luke, Doernberg, Sarah B, Jay, Cheryl A, Douglas, Vanja, Josephson, S Andrew, DeRisi, Joseph L   +31 more
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