Results 51 to 60 of about 4,937,404 (296)

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Two Human Cases of Fatal Meningoencephalitis Associated with Potosi and Lone Star Virus Infections, United States, 2020–2023

Emerging Infectious Diseases
We used clinical metagenomic next-generation sequencing of cerebrospinal fluid to investigate bunyavirus infections in 2 immunocompromised patients in the United States who had fatal meningoencephalitis.
Charles Y. Chiu, Raja Rama Godasi, Holly R. Hughes, Venice Servellita, Kafaya Foresythe, Asritha Tubati, Kelsey Zorn, Sukhman Sidhu, Michael R. Wilson, Sai Varun Bethina, Daniel Abenroth, Yee Cheng, Raymond Grams, Camilla Reese, Carlos Isada, Neeharika Thottempudi   +15 more
doaj   +1 more source

Metagenomic next-generation sequencing as a diagnostic tool for toxoplasmic encephalitis [PDF]

Annals of Clinical Microbiology and Antimicrobials, 2018
More than 100 different pathogens can cause encephalitis. Testing of all the neurological pathogens by conventional methods can be difficult. Metagenomic next-generation sequencing (NGS) could identify the infectious agents in a target-independent manner. The role of this novel method in clinical diagnostic microbiology still needs to be evaluated.
Zhiliang Hu, Xing Weng, Chunhua Xu, Yang Lin, Cong Cheng, Hongxia Wei, Wei Chen   +6 more
openaire   +3 more sources

Diagnostic applications of next generation sequencing: working towards quality standards [PDF]

, 2012
Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Klein, Hanns-Georg, Biskup, S., Vogl, Ina, Hirv, K., Greif, P.A., Kotschote, S., Gehring, A., Kuhn, M., Stuhrmann, Manfred, Bergmann, C., Eck, Sebastian H., Biskup, Saskia, Metzeler, Klaus H., Greif, Philipp A., Stuhrmann, M., Bolz, Hanno Joern, Benet-Pages, Anna, Hirv, Kaimo, Benet-Pagès, A., Klein, H.G., Vogl, I., Bolz, H.J., Kuhn, Marius, Kotschote, Stefan, Gehring, Andrea, Bergmann, Carsten, Metzeler, K.H., Eck, S.H.   +27 more
core   +1 more source

Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

FEBS Open Bio, EarlyView.
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi, Alain Dolla, Frauke Baymann, Celine Rommevaux, Latifa Elantak, Helene Gaussier, Matthieu Nouailler   +6 more
wiley   +1 more source

Case report: Chronic Candida albicans meningitis: a rare entity diagnosed by metagenomic next-generation sequencing. [PDF]

The aetiology of chronic aseptic meningitis is difficult to establish. Candida meningitis in particular is often diagnosed late, as cerebrospinal fluid (CSF) work-up and imaging findings are nonspecific.
Lazarevic, Vladimir, Z'Graggen, Werner J, Müller, Mandy D, Walti, Laura N, Schrenzel, Jacques, Oberli, Alexander, Martin, Yonas, Z Graggen, Werner J, Kuenzli, Andrea B   +8 more
core   +2 more sources

Targeted Restoration of the Microbial Tryptophan Oxidative Pathway Ameliorates Autism‐Like Behavioral and Synaptic Deficits

Advanced Science, EarlyView.
ABSTRACT Autism Spectrum Disorder (ASD) is associated with gut microbiota dysbiosis, yet the contribution of specific microbial metabolic pathways remains unclear. Reanalysis of a public ASD cohort revealed reduced functional potential in microbial tryptophan oxidative metabolism.
Rui Guo, Caian He, Xiao Xiao, Jingmeng Li, Zilong Zhang, Ying He, Chuanchuan Wang, Jiale Zhao, Jun Gong, Jiarui Liang, Tian Yuan, Haiting Sun, Xuebo Liu, Chao Gao, Zhigang Liu   +14 more
wiley   +1 more source

Employing Metagenomics Capture targeted next-generation sequencing for the etiological diagnosis of bloodstream infections

Frontiers in Cellular and Infection Microbiology
BackgroundBloodstream infections (BSIs) represent a significant public health concern. Metagenomic Capture targeted next-generation sequencing technology, as a newly emerging method for pathogen detection, has been applied in the etiological diagnosis of
Chuyu Lao, Zihuan Li, Guanwen Lin, Congrong Li, Yuqi Wang   +4 more
doaj   +1 more source

Metagenomic Next-Generation Sequencing for the Diagnosis of Neonatal Infectious Diseases

Microbiology Spectrum, 2022
To the best of our knowledge, this is the first report on evaluating the performance of mNGS using cfDNA and RNA from body fluid and blood samples for diagnosing neonatal infections. mNGS of RNA and cfDNA can achieve the unbiased detection and identification of trace pathogens from different kinds of neonatal body fluid and blood samples with a high ...
Lu Chen, Yujuan Zhao, Jiakai Wei, Wendi Huang, Ying Ma, Xuefeng Yang, Yang Liu, Jing Wang, Han Xia, Zheng Lou   +9 more
openaire   +3 more sources

High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]

, 2010
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P., Terauchi, Ryohei, Hideo Matsumura, Krüger Detlev H., Kentaro Yoshida, Kahl, G., Takahiro Fujibe, Luo, Shujun, Fujibe, T., Terauchi Ryohei, Eiji Kimura, Yoshida Kentaro, Luo, S., Shujun Luo, Barrero, R.A., Schroth, Gary P., Detlev H Krüger, Albertyn, Z., Ryohei Terauchi, Roberto A Barrero, Kahl Günter, Luo Shujun, Albertyn Zayed, Gary P. Schroth, Matsumura, Hideo, Yoshida, K., Fujibe, Takahiro, Schroth Gary P., Krüger, D.H., Kahl, Günter, Krüger, Detlev H., Kimura, Eiji, Gary P Schroth, Matsumura Hideo, Barrero Roberto A., Zayed Albertyn, Kimura, E., Terauchi, R., Kimura Eiji, Detlev H. Krüger, Albertyn, Zayed, Matsumura, H., Yoshida, Kentaro, Fujibe Takahiro, Barrero, Roberto A., Roberto A. Barrero, Günter Kahl, Kahl, Guenter, Schroth, Gary P, Krueger, Detlev H, Barrero, Roberto A   +50 more
core   +1 more source