Results 51 to 60 of about 4,663,034 (296)

Human Pegivirus Encephalitis With Brain Detection and Response to Sofosbuvir Ledipasvir

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Human pegivirus (HPgV‐1) has been associated with severe encephalomyelitis in immunocompromised patients. Its neurological spectrum remains poorly defined. We report a slowly progressive encephalitis in a person living with well‐controlled HIV, characterized by white matter abnormalities and inflammatory cerebrospinal fluid (CSF). HPgV RNA was
Antoine Moulignier, Françoise Heran, Francis Lallemand, Pierre Bourdillon   +3 more
wiley   +1 more source

Polychip‐A High‐Throughput Droplet Microfluidics Platform for Interrogating Microbial Interactions

Advanced Science, EarlyView.
Polychip, a fully integrated droplet microfluidics platform, enables high‐throughput, single‐cell resolution screening of polymicrobial interactions. By seamlessly combining six automated microfluidics operations on a single chip, the system accelerates antimicrobial discovery by 11 to 14 times compared to traditional robotic methods.
Jeong Jae Han, Adrian Ryan Guzman, Aifen Zhou, Han Zhang, Rohit Gupte, Haemin Jung, Kaylee Delgado, Sini Skariah, Ali Sultan, Arul Jayaraman, Paul de Figueiredo, Arum Han   +11 more
wiley   +1 more source

Employing Metagenomics Capture targeted next-generation sequencing for the etiological diagnosis of bloodstream infections

Frontiers in Cellular and Infection Microbiology
BackgroundBloodstream infections (BSIs) represent a significant public health concern. Metagenomic Capture targeted next-generation sequencing technology, as a newly emerging method for pathogen detection, has been applied in the etiological diagnosis of
Chuyu Lao, Zihuan Li, Guanwen Lin, Congrong Li, Yuqi Wang   +4 more
doaj   +1 more source

Application of metagenomic next-generation sequencing in patients with infective endocarditis

Frontiers in Cellular and Infection Microbiology, 2023
ObjectivesMetagenomic next-generation sequencing (mNGS) technology is helpful for the early diagnosis of infective endocarditis, especially culture-negative infective endocarditis, which may guide clinical treatment. The purpose of this study was to compare the presence of culture-negative infective endocarditis pathogens versus culture-positive ones ...
Shao-Lin Li, Xi Zhao, Jun-Zhong Tao, Zhen-Zhen Yue, Xiao-Yan Zhao   +4 more
openaire   +3 more sources

QTrim : a novel tool for the quality trimming of sequence reads generated using the Roche/454 sequencing platform [PDF]

, 2014
Background Many high throughput sequencing (HTS) approaches, such as the Roche/454 platform, produce sequences in which the quality of the sequence (as measured by a Phred-like quality scores) decreases linearly across a sequence read.
Grace P McCormack, Lubinsky, Baruch, Bansode, Vijay B., Mónica BJ Moinz, McCormack, Grace P., Vijay B Bansode, Travers, Simon A., Simon A Travers, Ram Shrestha, Baruch Lubinsky, Shrestha, Ram, Moinz, Mónica B. J.   +11 more
core   +1 more source

Comprehensive Detection of Candidate Pathogens in the Lower Respiratory Tract of Pediatric Patients With Unexpected Cardiopulmonary Deterioration Using Next-Generation Sequencing [PDF]

, 2020
Objectives: Next-generation sequencing has been applied to the investigation of microorganisms in several clinical settings. We investigated the infectious etiologies in respiratory specimens from pediatric patients with unexpected cardiopulmonary ...
Suzuki, Takako, Kazuhiro Horiba, Makoto Yamaguchi, Jun-ichi Kawada, Horiba, Kazuhiro, Toshihiko Okumura, Takanari Ikeyama, Okumura, Toshihiko, Ikeyama, Takanari, Shinji Kawabe, Kawabe, Shinji, Ito, Yoshinori, Yuka Torii, Takeuchi, Suguru, Kawada, Jun-ichi, Sho Wada, Yamaguchi, Makoto, Torii, Yuka, Yoshinori Ito, Suguru Takeuchi, Wada, Sho, Takako Suzuki   +21 more
core   +1 more source

RNAi in the Rhizarian Phytopathogen Plasmodiophora brassicae: The Causal Agent of Clubroot Disease in Cruciferous Crops

Advanced Science, EarlyView.
This study uncovers an unusual RNAi pathway in the rhizarian pathogen Plasmodiophora brassicae. In the absence of Dicer, a Drosha‐like RNase III protein supports the biogenesis of predominant 21‐nt small RNAs, and two Argonaute proteins mediate small RNA‐guided silencing.
Xiong Zhang, Yupo Wu, Jiachang Hou, Lixia Gao, Longqi Pan, Cuicui Shen, Chuanji Zhao, Feng Gao, Yi Zhang, Li Ren, Xiaoping Fang, Xiaoling Dun, Shengyi Liu, Lijiang Liu   +13 more
wiley   +1 more source

Detection of Neoplasms by Metagenomic Next-Generation Sequencing of Cerebrospinal Fluid

JAMA Neurology, 2021
Cerebrospinal fluid (CSF) cytologic testing and flow cytometry are insensitive for diagnosing neoplasms of the central nervous system (CNS). Such clinical phenotypes can mimic infectious and autoimmune causes of meningoencephalitis.To ascertain whether CSF metagenomic next-generation sequencing (mNGS) can identify aneuploidy, a hallmark of malignant ...
Gu, Wei, Rauschecker, Andreas M, Hsu, Elaine, Zorn, Kelsey C, Sucu, Yasemin, Federman, Scot, Gopez, Allan, Arevalo, Shaun, Sample, Hannah A, Talevich, Eric, Nguyen, Eric D, Gottschall, Marc, Nourbakhsh, Bardia, Gold, Carl A, Cree, Bruce AC, Douglas, Vanja C, Richie, Megan B, Shah, Maulik P, Josephson, S Andrew, Gelfand, Jeffrey M, Miller, Steve, Wang, Linlin, Tihan, Tarik, DeRisi, Joseph L, Chiu, Charles Y, Wilson, Michael R   +25 more
openaire   +4 more sources

Virtual Environment for Next Generation Sequencing Analysis [PDF]

, 2012
Next Generation Sequencing technology, on the one hand, allows a more accurate analysis, and, on the other hand, increases the amount of data to process.
Provenzano, R., Provenzano R., Terzo, Olivier, Mossucca, L., Acquaviva, Andrea, Mossucca L, Abate, Francesco   +6 more
core  

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source