Results 71 to 80 of about 6,252 (250)

Metagenomic Next-Generation Sequencing Assists in the Diagnosis of Mediastinal Aspergillus fumigatus Abscess in an Immunocompetent Patient: A Case Report and Literature Review

, 2023
Wusheng Deng,1,* Yun Jiang,2,* Jiaoxia Qin,1,* Gang Chen,3,* Yongjie Lv,1 Yanmei Lei,1 Jing Luo,1 Kangkang Hong,1 Bing Huang,1 Luhai Qin,4 Xiujia Tang,1 Liumei Ye,1 Yuhai Dang,1 Chao Wang,1 Feiyang Long,1 Ke Wang,1 Jinliang Kong1 ...
Long F, Qin L, Qin J, Wang C, Lv Y, Hong K, Chen G, Kong J, Wang K, Jiang Y, Ye L, Luo J, Lei Y, Dang Y, Deng W, Huang B, Tang X   +16 more
core  

Clinical Efficiency of Metagenomic Next-Generation Sequencing in Sputum for Pathogen Detection of Patients with Pneumonia According to Disease Severity and Host Immune Status

, 2023
Can Chang,1 Huan Wang,1 Lianjun Zhang,1 Junling Hao,1 Xiaoning Wang,1 Yaoyao Wang,2 Fei Qi,2 Jingwei Lou,2 Jiangman Zhao,2 Junying Dong1 1Department of Respiratory and Critical Care Medicine, Shandong Provincial Hospital Heze Branch, Heze, Shandong ...
Hao J, Dong J, Qi F, Chang C, Wang H, Zhang L, Wang X, Wang Y, Zhao J, Lou J   +9 more
core  

Neurobrucellosis: Unexpected Answer From Metagenomic Next-Generation Sequencing. [PDF]

, 2017
A diagnosis of brucellosis can be difficult because routine culture and serological methods exhibit variable sensitivity and specificity. We present the use of a metagenomic next- generation sequencing assay to diagnose a case of neurobrucellosis from ...
Yu, Guixia, Miller, Steve, Mongkolrattanothai, Kanokporn, Samayoa, Erik, Chiu, Charles Y, Bender, Jeffrey M, Dien Bard, Jennifer, Naccache, Samia N, Pham, Elizabeth, Aldrovandi, Grace   +9 more
core   +1 more source

MFPD: A Multiple Fungal Pathogen Detection Pipeline Across Diverse Habitats

Advanced Science, EarlyView.
The MFPD pipeline integrates a comprehensive ITS reference database of fungal pathogens, optimized parameters, and algorithms tailored for both full‐length and subregion sequences that balance accuracy and computational efficiency; it enables high‐throughput, species‐level identification from amplicon sequencing data, supporting large‐scale ...
Yi Shen, Xinrun Yang, Jiabao Yu, Yaozhong Zhang, Tianjie Yang, Yang Gao, Xiaofang Wang, Alexandre Jousset, Waseem Raza, Fang‐Jie Zhao, Qirong Shen, Gaofei Jiang, Zhong Wei, Yangchun Xu   +13 more
wiley   +1 more source

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

Case report: Chronic Candida albicans meningitis: a rare entity diagnosed by metagenomic next-generation sequencing. [PDF]

The aetiology of chronic aseptic meningitis is difficult to establish. Candida meningitis in particular is often diagnosed late, as cerebrospinal fluid (CSF) work-up and imaging findings are nonspecific.
Lazarevic, Vladimir, Z'Graggen, Werner J, Müller, Mandy D, Walti, Laura N, Schrenzel, Jacques, Oberli, Alexander, Martin, Yonas, Z Graggen, Werner J, Kuenzli, Andrea B   +8 more
core   +1 more source

A Dual‐Functional Biohybrid Nanorobot to Synergistically Eradicate Biofilm and Degrade Antibiotic Resistance Genes

Advanced Science, EarlyView.
A biohybrid nanorobot integrating lytic bacteriophage N4 with Pd nanozymes is developed for targeted eradication of multidrug‐resistant E. coli biofilms. Synergistic bacterial lysis and ROS‐mediated oxidation enable simultaneous biofilm removal and antibiotic resistance genes degradation, maintaining high efficacy in complex wastewater environments ...
Junzheng Zhang, Tong Dou, Luokai Wang, Jingxian Yang, Wenrui Wang, Shuaitian Guo, Xiangyu Li, Xuefang Huang, Yunlei Xianyu, Dongsheng Wang   +9 more
wiley   +1 more source

The diagnostic value of metagenomic next⁃generation sequencing in infectious diseases

BMC Infectious Diseases, 2021
Background Although traditional diagnostic techniques of infection are mature and price favorable at present, most of them are time-consuming and with a low positivity.
Hongxia Duan, Xuan Li, Aihong Mei, Ping Li, Yang Liu, Xiaofeng Li, Weiwei Li, Changhui Wang, Shuanshuan Xie   +8 more
doaj   +1 more source

Sulfated Cyclocarya Paliurus Polysaccharide Sorchestrates the Gut Microbiome to Mobilize a Host‐Derived 12‐HEPE Against Ulcerative Colitis

Advanced Science, EarlyView.
A sulfated polysaccharide (SCP) from Cyclocarya paliurus alleviates ulcerative colitis by reshaping the gut microbiome. This remodeled microbiota orchestrates the upregulation of the host‐derived lipid 12‐HEPE, which directly inhibits the TLR4/NF‐κB pathway to suppress inflammation.
Xianxiang Chen, Mingyue Shen, Rui Zhang, Zhibing Huang, Hui Niu, Qiang Yu, Yi Chen, Xiangwen Pan, Liyuan Rong, Huiliang Wen, Jun Yang, Jianhua Xie   +11 more
wiley   +1 more source

Special features of RAD Sequencing data:implications for genotyping

, 2012
Restriction site-associated DNA Sequencing (RAD-Seq) is an economical and efficient method for SNP discovery and genotyping. As with other sequencing-by-synthesis methods, RAD-Seq produces stochastic count data and requires sensitive analysis to develop ...
Fuentes-Utrilla, Pablo, Blaxter, Mark L, Pablo Fuentes-utrilla, Karim Gharbi, Davey, John W, Cezard, Timothée, John W. Davey, Gharbi, Karim, Pablo Fuentes‐Utrilla, Cathlene Eland, Timothée Cezard, Mark L. Blaxter, Eland, Cathlene   +12 more
core   +1 more source