Results 21 to 30 of about 6,252 (250)

Polymicrobial Anaerobic Meningitis Detected by Next-Generation Sequencing: Case Report and Review of the Literature

Frontiers in Medicine, 2022
BackgroundAnaerobic meningitis is a severe central nervous system infection associated with significant neurological sequelae and high mortality. However, the precise detection of causative pathogen(s) remains difficult because anaerobic bacteria are ...
Xiaoqiang Li, Hui Du, Zhibin Song, Hui Wang, Xiong Long   +4 more
doaj   +1 more source

Epileptic Seizure after Use of Moxifloxacin in Man with Legionella longbeachae Pneumonia

Emerging Infectious Diseases, 2020
Legionellosis caused by Legionella longbeachae is diagnosed mainly by PCR. We report a case of L. longbeachae infection in mainland China, which was diagnosed by metagenomic next-generation sequencing, in a man who developed an epileptic seizure after ...
Jin-Yong Wang, Xing Li, Jian-Yong Chen, Bo Tong   +3 more
doaj   +1 more source

Emergomyces orientalis Emergomycosis Diagnosed by Metagenomic Next-Generation Sequencing

Emerging Infectious Diseases, 2021
Emergomyces is a newly described dimorphic fungus genus; it may cause fatal infections in immunocompromised patients, but diagnosis is often delayed. We report a case of disseminated emergomycosis caused by the novel species Emergomyces orientalis in a ...
Da He, Min Quan, Hongyan Zhong, Zhixing Chen, Xioahui Wang, Fang He, Junyan Qu, Taoyou Zhou, Xiaoju Lv, Zhiyong Zong   +9 more
doaj   +1 more source

Pneumocystis Jirovecii Pneumonia Diagnosis via Metagenomic Next-Generation Sequencing

Frontiers in Medicine, 2022
The incidence of non-HIV-infected Pneumocystis Jirovecii Pneumonia (PJP) is increasing. The prognosis for non-HIV PJP is poor and diagnostic tests are of lower sensitivity in non-HIV patients.
Xiaoxiao Lu, Jianhui Zhang, Jianhui Zhang, Wentao Ma, Lihua Xing, Hanbing Ning, Mengying Yao   +6 more
doaj   +1 more source

Metagenomic Next-Generation Sequencing for Periprosthetic Joint Infections

Cureus, 2023
Periprosthetic joint infection (PJI) after arthroplasty is a major complication, which requires significant resources, resulting in high costs for the medical system. In recent years, significant progress has been made in the diagnosis and treatment of periprosthetic infections, the identification of the pathogen being the central element in the ...
Cretu, Bogdan, Iordache, Sergiu, Cursaru, Adrian, Serban, Bogdan, Costache, Mihai, Cirstoiu, Catalin, Spiridonica, Razvan   +6 more
openaire   +2 more sources

NeSSM: A Next-Generation Sequencing Simulator for Metagenomics

PLoS ONE, 2013
Metagenomics can reveal the vast majority of microbes that have been missed by traditional cultivation-based methods. Due to its extremely wide range of application areas, fast metagenome sequencing simulation systems with high fidelity are in great demand to facilitate the development and comparison of metagenomics analysis tools.We present here a ...
Ben Jia, Liming Xuan, Kaiye Cai, Zhiqiang Hu, Liangxiao Ma, Chaochun Wei   +5 more
openaire   +4 more sources

Can metagenomic next-generation sequencing identify the pathogens responsible for culture-negative prosthetic joint infection?

BMC Infectious Diseases, 2020
Background The aims of this study were to (1) evaluate the efficacy and safety of targeted antibiotics for the treatment of culture-negative prosthetic joint infection based on metagenomic next-generation sequencing results and (2) verify the accuracy ...
Chaoxin Wang, Zida Huang, Wenbo Li, Xinyu Fang, Wenming Zhang   +4 more
doaj   +1 more source

Metagenomic Next-generation Sequencing: Current Status, Challenges and Prospects of Clinical Application

Xiehe Yixue Zazhi, 2023
Traditional microbial detection methods have difficulty meeting the needs of pathogen detection in the clinic. In recent years, metagenomic next-generation sequencing (mNGS) technology has emerged and been widely used in the diagnosis of various ...
DIAO Zhenli, LI Jinming
doaj   +1 more source

From cheek swabs to consensus sequences : an A to Z protocol for high-throughput DNA sequencing of complete human mitochondrial genomes [PDF]

, 2014
Background: Next-generation DNA sequencing (NGS) technologies have made huge impacts in many fields of biological research, but especially in evolutionary biology. One area where NGS has shown potential is for high-throughput sequencing of complete mtDNA
Matisoo-Smith, Elizabeth A, Soria Hernanz, David F, Schurr, Theodore G., Stanton, Jo-Ann, Martínez-Cruz, Begoña, White, W. T., GaneshPrasad, Arun Kumar, Lacerda, Daniela R., Matisoo Smith, Elizabeth, White, W. Timothy J., Vilar, Miguel G., Platt, Daniel E., Parida, Laxmi, Hernanz, Soria, Martínez Cruz, Begoña, Kaplan, Matthew E., Clarke, Andrew, Schurr, Theodore G, Matisoo-Smith, Lisa, Matisoo-Smith, Elizabeth, Mitchell, R. John, Matthew E Kaplan, Vieira, Pedro Paulo, Jo-Ann L Stanton, Matisoo-Smith, Elizabeth A., Dulik, Matthew C., Zalloua, Pierre A., Kaplan, Matthew, Tyler-Smith, Chris, Kaplan, M., W Timothy J White, Santhakumari, Arun Varatharajan, Haak, Wolfgang, Quintana-Murci, Lluis, Stefan Prost, null null, Comas, David, Ziegle, Janet S., Swamikrishnan, Pandikumar, Kaplan, Matthew E, Wells, R. Spencer, HASH(0x55a67fb2a340), White, W Timothy J, Cooper, Alan, Clarke, Andrew C, Der Sarkissian, Clio S.I., The, Genographic Consortium, Stanton, J., Bertranpetit, Jaume, 1952-, White, W Timothy J., Stanton, Jo-Ann L., Stanton, Jo Ann L., Pitchappan, Ramasamy, Adhikarla, Syama, Santos, Fabrício R., Comas, David, 1969-, Haber, Marc; id_orcid, Soodyall, Himla, Royyuru, Ajay K., Prost, Stefan, Adler, Christina J., Renfrew, Colin, Jin, Li, Clark, Andrew, Elizabeth A Matisoo-Smith, Andrew C Clarke, Matisoo-Smith, E., Li, Shilin, The Genographic Consortium, Balanovsky, Oleg, Balanovska, Elena, Clarke, Andrew C., Prost, S., Merchant, Nirav C., Andrew C Clarke, Owings, Amanda C., Stanton, Jo-Ann L, White, W., Bertranpetit, Jaume, Gaieski, Jill B., Genographic Consortium, Haber, Marc, 1980-, Genographic Consortium,, Clarke, A.   +83 more
core   +1 more source

Metagenomic Next-Generation Sequencing Improves Diagnosis of Osteoarticular Infections From Abscess Specimens: A Multicenter Retrospective Study

Frontiers in Microbiology, 2020
Background: We conducted this retrospective study to reveal the accuracy of metagenomic next-generation sequencing (mNGS) for diagnosing osteoarticular infections from fresh abscess specimens obtained from patients in an HIV-naive population.Methods: We ...
Mingwei Zhao, Kai Tang, Fengsheng Liu, Weidong Zhou, Jun Fan, Guangxuan Yan, Shibing Qin, Yu Pang   +7 more
doaj   +1 more source