Results 21 to 30 of about 30,985 (257)
Xiehe Yixue Zazhi, 2023 Traditional microbial detection methods have difficulty meeting the needs of pathogen detection in the clinic. In recent years, metagenomic next-generation sequencing (mNGS) technology has emerged and been widely used in the diagnosis of various ...
DIAO Zhenli, LI Jinming
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Frontiers in Microbiology, 2020 Background: We conducted this retrospective study to reveal the accuracy of metagenomic next-generation sequencing (mNGS) for diagnosing osteoarticular infections from fresh abscess specimens obtained from patients in an HIV-naive population.Methods: We ...
Mingwei Zhao +7 more
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Anncaliia algerae Microsporidiosis Diagnosed by Metagenomic Next-Generation Sequencing, China
Emerging Infectious Diseases, 2022 We report a case of Anncaliia algerae microsporidia infection in an immunosuppressed kidney transplant recipient in China. Light microscopy and transmission electron microscopy initially failed to identify A.
Chen Liu +3 more
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The Role of Metagenomics and Next-Generation Sequencing in Infectious Disease Diagnosis [PDF]
Clinical Chemistry, 2021 Abstract Background Metagenomic next-generation sequencing (mNGS) for pathogen detection is becoming increasingly available as a method to identify pathogens in cases of suspected infection. mNGS analyzes the nucleic acid content of patient samples with high-throughput sequencing technologies to ...
Steve, Miller, Charles, Chiu
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International Journal of Infectious Diseases, 2020 Objectives: Pathogen identification is crucial for the diagnosis and management of periprosthetic joint infection (PJI). Although culturing methods are the foundation of pathogen detection in PJI, false-negative results often occur.
Chao-xin Wang +12 more
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BMC Infectious Diseases, 2022 Background The purpose of this study was to evaluate different pretreatment, extraction, amplification, and library generation methods for metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) and to develop an efficient procedure ...
Hanfang Jiang +10 more
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Assessment of metagenomic assembly using simulated next generation sequencing data. [PDF]
PLoS ONE, 2012 Due to the complexity of the protocols and a limited knowledge of the nature of microbial communities, simulating metagenomic sequences plays an important role in testing the performance of existing tools and data analysis methods with metagenomic data ...
Daniel R Mende +7 more
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Metagenomic next-generation sequencing in the diagnosis of neurocysticercosis: A case report
World Journal of Clinical Cases, 2023 Inflammatory myofibroblastic tumor (IMT) of the biliary tract is rare, and often difficult to diagnose or to distinguish from other tumors due to its atypical clinical presentation and nonspecific radiological features. Histologically, IMTs are (myo)fibroblastic neoplasms with a prominent inflammatory infiltrate.
Xu, Wen-Bin +9 more
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The Early Diagnosis of Scrub Typhus by Metagenomic Next-Generation Sequencing [PDF]
Frontiers in Public Health, 2021 Introduction: Scrub typhus is a mite-borne infection widespread in Southeast Asia, with clinical symptoms such as fever, chills, skin rash, eschar at the bite site, and other signs of acute febrile illness. The Rickettsia pathogen (Orientia tsutsugamushi) is always difficult to be diagnosed at an early stage by traditional clinical diagnostic methods ...
Xianghong Liu +5 more
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Infection and Drug Resistance, 2022 Meng Zhang,1,* Wenyi Lu,2,* Danni Xie,1 Jiaxi Wang,1 Xia Xiao,2 Yedi Pu,2 Juanxia Meng,2 Hairong Lyu,2 Mingfeng Zhao2 1First Center Clinic College of Tianjin Medical University, Tianjin, 300192, People’s Republic of China; 2Department of ...
Zhang M +8 more
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