Results 61 to 70 of about 7,727 (208)

Arbuscular mycorrhizal fungal community abundance, functions, and symbiotic interactions revealed by root metatranscriptomes

iMetaOmics, EarlyView.
Paradigm shift: PCR‐free methods reveal 6–15‐fold higher arbuscular mycorrhizal (AM) fungal abundance than metabarcoding, exposing systematic underestimation across decades of research. Predictive power: AM fungal abundance serves as a community‐level trait that predicts crop yield under drought conditions.
Peilin Chen, John W. Taylor, Cheng Gao
wiley   +1 more source

SARS‐CoV‐2 triggered oxidative stress and abnormal energy metabolism in gut microbiota

MedComm, 2022
Specific roles of gut microbes in COVID‐19 progression are critical. However, the circumstantial mechanism remains elusive. In this study, shotgun metagenomic or metatranscriptomic sequencing was performed on fecal samples collected from 13 COVID‐19 ...
Tuoyu Zhou, Jingyuan Wu, Yufei Zeng, Junfeng Li, Jun Yan, Wenbo Meng, Huawen Han, Fengya Feng, Jufang He, Shuai Zhao, Ping Zhou, Ying Wu, Yanlin Yang, Rong Han, Weilin Jin, Xun Li, Yunfeng Yang, Xiangkai Li   +17 more
doaj   +1 more source

A Pilot Cross-Sectional Study of Immunological and Microbiome Profiling Reveals Distinct Inflammatory Profiles for Smokers, Electronic Cigarette Users, and Never-Smokers

Microorganisms, 2023
Smokers (SM) have increased lung immune cell counts and inflammatory gene expression compared to electronic cigarette (EC) users and never-smokers (NS).
Peter G. Shields, Kevin L. Ying, Theodore M. Brasky, Jo L. Freudenheim, Zihai Li, Joseph P. McElroy, Sarah A. Reisinger, Min-Ae Song, Daniel Y. Weng, Mark D. Wewers, Noah B. Whiteman, Yiping Yang, Ewy A. Mathé   +12 more
doaj   +1 more source

A predator-prey interaction between a marine Pseudoalteromonas sp. and Gram-positive bacteria [PDF]

, 2020
Predator-prey interactions play important roles in the cycling of marine organic matter. Here we show that a Gram-negative bacterium isolated from marine sediments (Pseudoalteromonas sp.
Chen, Xiu-Lan, Chen, Yin, Ding, Haitao, Li, Chun-Yang, Lidbury, Ian, McMinn, Andrew, Su, Hai-Nan, Tang, Bai-Lu, Wang, Lei, Wang, Min, Wang, Peng, Yang, Jie, Yu, Yang, Zhang, Xi-Ying, Zhang, Yu-Zhong, Zhao, Hui-Lin, Zhong, Shuai   +16 more
core   +2 more sources

The role of the gut microbiome in the regulation of high‐altitude adaptation

iMeta, EarlyView.
This study is the first to elucidate mechanisms of high‐altitude adaptation from the perspective of the rumen ecosystem by using indigenous yaks and Holstein cows that have lived at high altitude since birth as comparative models. Through a systematic comparison of their rumen ecology using multi‐omics approaches—including rumen metagenomics ...
Xinyu Zhang, Senlin Zhu, Michael Kreuzer, Shoukun Ji, Wei Wang, Yanliang Bi, Shengli Li   +6 more
wiley   +1 more source

Metatranscriptome Analysis of Nasopharyngeal Swabs across the Varying Severity of COVID-19 Disease Demonstrated Unprecedented Species Diversity

Microorganisms, 2023
The recent global emergence of the SARS-CoV-2 pandemic has accelerated research in several areas of science whose valuable outputs and findings can help to address future health challenges in the event of emerging infectious agents.
Michaela Hyblova, Dominik Hadzega, Klaudia Babisova, Patrik Krumpolec, Andrej Gnip, Peter Sabaka, Stefan Lassan, Gabriel Minarik   +7 more
doaj   +1 more source

Accu16S/AccuITS: Accurate and broadly applicable amplicon sequencing for absolute microbiome quantification

iMeta, EarlyView.
Traditional 16S rRNA gene and Internal Transcribed Spacer region amplicon sequencing provides only relative abundance, often leading to biased ecological interpretations. To overcome this limitation, we developed Accu16S/AccuITS, an absolute quantification method for bacterial and fungal amplicons based on synthetic internal spike‐in DNA with known ...
Defeng Bai, Ou Fang, Caihua Li, Bin Cai, Xingyan Tan, Mengmeng Jiang, Bin Gan, Jinxia Fu, Yunyun Gao, Ying Wang, Yong‐Xin Liu   +10 more
wiley   +1 more source

Effective gene collection from the metatranscriptome of marine microorganisms [PDF]

, 2011
Background Metagenomic studies, accelerated by the evolution of sequencing technologies and the rapid development of genomic analysis methods, can reveal genetic diversity and biodiversity in various samples including those of uncultured or unknown ...
Atsushi Ogura, Mengjie Lin, Yuya Shigenobu, Atushi Fujiwara, Kazuho Ikeo, Satoshi Nagai   +5 more
core   +1 more source

Winter‐active spiders (Clubiona) have a hyperactive antifreeze protein with a unique beta‐solenoid fold

The FEBS Journal, EarlyView.
Antifreeze proteins from winter‐active spiders were purified using their affinity for ice. After LC–MSMS characterization, corresponding transcripts were identified. The antifreeze protein folds as a β‐solenoid with a large flat ice‐binding site on one surface and can bind to ice crystals and prevent their growth at −4 °C.
Laurie A. Graham, Stano Pekár, Ina M. Hainer, Peter L. Davies   +3 more
wiley   +1 more source

Metatranscriptome profiling of a harmful algal bloom [PDF]

Harmful Algae, 2014
Metagenomic methods provide a powerful means to investigate complex ecological phenomena. Developed originally for study of Bacteria and Archaea, the application of these methods to eukaryotic microorganisms is yet to be fully realized. Most prior environmental molecular studies of eukaryotes have relied heavily on PCR amplification with eukaryote ...
Endymion D, Cooper, Bastian, Bentlage, Theodore R, Gibbons, Tsvetan R, Bachvaroff, Charles F, Delwiche   +4 more
openaire   +2 more sources