Results 31 to 40 of about 9,285 (257)
Current and emerging plant diseases caused by obligate parasitic microbes such as rusts, downy mildews, and powdery mildews threaten worldwide crop production and food safety.
Li Guo +9 more
doaj +1 more source
Metatranscriptomic census of active protists in soils [PDF]
Abstract The high numbers and diversity of protists in soil systems have long been presumed, but their true diversity and community composition have remained largely concealed. Traditional cultivation-based methods miss a majority of taxa, whereas molecular barcoding approaches employing PCR introduce significant biases in reported ...
Geisen, S. +6 more
openaire +4 more sources
The Viral Fraction Metatranscriptomes of Lake Baikal
This article characterises viral fraction metatranscriptomes (smaller than 0.2 µm) from the pelagic zone of oligotrophic Lake Baikal (Russia). The study revealed the dominance of transcripts of DNA viruses: bacteriophages and algal viruses. We identified transcripts similar to Pithovirus sibericum, a nucleocytoplasmic large DNA virus (NCLDV) isolated ...
Sergey Potapov +5 more
openaire +3 more sources
Consistency of Targeted Metatranscriptomics and Morphological Characterization of Phytoplankton Communities [PDF]
The composition of phytoplankton community is the basis for environmental monitoring and assessment of the ecological status of aquatic ecosystems. Community composition studies of phytoplankton have been based on time-consuming and expertise-demanding ...
Tiirola, Marja +4 more
core +1 more source
Emerging infectious diseases in wildlife are responsible for massive population declines. In amphibians, chytridiomycosis caused by Batrachochytrium dendrobatidis, Bd, has severely affected many amphibian populations and species around the world.
Eria Alaide Rebollar +19 more
doaj +1 more source
Regulatory Noncoding Small RNAs Are Diverse and Abundant in an Extremophilic Microbial Community
Regulatory small RNAs (sRNAs) play large-scale and essential roles in many cellular processes across all domains of life. Microbial sRNAs have been extensively studied in model organisms, but very little is known about the dynamics of sRNA synthesis and ...
Diego R. Gelsinger +5 more
doaj +1 more source
Microbial communities play integral roles in driving nutrient and energy transformations in the ocean, collectively contributing to fundamental biogeochemical cycles. Although it is well known that these communities are stratified within the water column,
Maria A. Cavaco +12 more
doaj +1 more source
DNA/RNA Preservation in Glacial Snow and Ice Samples
The preservation of nucleic acids for high-throughput sequencing is an ongoing challenge for field scientists. In particular, samples that are low biomass, or that have to be collected and preserved in logistically challenging environments (such as ...
Christopher B. Trivedi +10 more
doaj +1 more source
Advances and Challenges in Metatranscriptomic Analysis
Sequencing-based analyses of microbiomes have traditionally focused on addressing the question of community membership and profiling taxonomic abundance through amplicon sequencing of 16 rRNA genes. More recently, shotgun metagenomics, which involves the random sequencing of all genomic content of a microbiome, has dominated this arena due to ...
Migun Shakya +2 more
openaire +3 more sources
MetaTrans: an open-source pipeline for metatranscriptomics [PDF]
AbstractTo date, meta-omic approaches use high-throughput sequencing technologies, which produce a huge amount of data, thus challenging modern computers. Here we present MetaTrans, an efficient open-source pipeline to analyze the structure and functions of active microbial communities using the power of multi-threading computers.
Martinez, Xavier +8 more
openaire +3 more sources

