Results 31 to 40 of about 9,285 (257)

A de-novo-assembly-based Data Analysis Pipeline for Plant Obligate Parasite Metatranscriptomic Studies

open access: yesFrontiers in Plant Science, 2016
Current and emerging plant diseases caused by obligate parasitic microbes such as rusts, downy mildews, and powdery mildews threaten worldwide crop production and food safety.
Li Guo   +9 more
doaj   +1 more source

Metatranscriptomic census of active protists in soils [PDF]

open access: yesThe ISME Journal, 2015
Abstract The high numbers and diversity of protists in soil systems have long been presumed, but their true diversity and community composition have remained largely concealed. Traditional cultivation-based methods miss a majority of taxa, whereas molecular barcoding approaches employing PCR introduce significant biases in reported ...
Geisen, S.   +6 more
openaire   +4 more sources

The Viral Fraction Metatranscriptomes of Lake Baikal

open access: yesMicroorganisms, 2022
This article characterises viral fraction metatranscriptomes (smaller than 0.2 µm) from the pelagic zone of oligotrophic Lake Baikal (Russia). The study revealed the dominance of transcripts of DNA viruses: bacteriophages and algal viruses. We identified transcripts similar to Pithovirus sibericum, a nucleocytoplasmic large DNA virus (NCLDV) isolated ...
Sergey Potapov   +5 more
openaire   +3 more sources

Consistency of Targeted Metatranscriptomics and Morphological Characterization of Phytoplankton Communities [PDF]

open access: yes, 2020
The composition of phytoplankton community is the basis for environmental monitoring and assessment of the ecological status of aquatic ecosystems. Community composition studies of phytoplankton have been based on time-consuming and expertise-demanding ...
Tiirola, Marja   +4 more
core   +1 more source

Using omics and integrated multi-omics approaches to guide probiotic selection to mitigate chytridiomycosis and other emerging infectious diseases

open access: yesFrontiers in Microbiology, 2016
Emerging infectious diseases in wildlife are responsible for massive population declines. In amphibians, chytridiomycosis caused by Batrachochytrium dendrobatidis, Bd, has severely affected many amphibian populations and species around the world.
Eria Alaide Rebollar   +19 more
doaj   +1 more source

Regulatory Noncoding Small RNAs Are Diverse and Abundant in an Extremophilic Microbial Community

open access: yesmSystems, 2020
Regulatory small RNAs (sRNAs) play large-scale and essential roles in many cellular processes across all domains of life. Microbial sRNAs have been extensively studied in model organisms, but very little is known about the dynamics of sRNA synthesis and ...
Diego R. Gelsinger   +5 more
doaj   +1 more source

Pathway-Centric Analysis of Microbial Metabolic Potential and Expression Along Nutrient and Energy Gradients in the Western Atlantic Ocean

open access: yesFrontiers in Marine Science, 2022
Microbial communities play integral roles in driving nutrient and energy transformations in the ocean, collectively contributing to fundamental biogeochemical cycles. Although it is well known that these communities are stratified within the water column,
Maria A. Cavaco   +12 more
doaj   +1 more source

DNA/RNA Preservation in Glacial Snow and Ice Samples

open access: yesFrontiers in Microbiology, 2022
The preservation of nucleic acids for high-throughput sequencing is an ongoing challenge for field scientists. In particular, samples that are low biomass, or that have to be collected and preserved in logistically challenging environments (such as ...
Christopher B. Trivedi   +10 more
doaj   +1 more source

Advances and Challenges in Metatranscriptomic Analysis

open access: yesFrontiers in Genetics, 2019
Sequencing-based analyses of microbiomes have traditionally focused on addressing the question of community membership and profiling taxonomic abundance through amplicon sequencing of 16 rRNA genes. More recently, shotgun metagenomics, which involves the random sequencing of all genomic content of a microbiome, has dominated this arena due to ...
Migun Shakya   +2 more
openaire   +3 more sources

MetaTrans: an open-source pipeline for metatranscriptomics [PDF]

open access: yesScientific Reports, 2016
AbstractTo date, meta-omic approaches use high-throughput sequencing technologies, which produce a huge amount of data, thus challenging modern computers. Here we present MetaTrans, an efficient open-source pipeline to analyze the structure and functions of active microbial communities using the power of multi-threading computers.
Martinez, Xavier   +8 more
openaire   +3 more sources

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