Results 31 to 40 of about 7,593 (210)
The Viral Fraction Metatranscriptomes of Lake Baikal
Microorganisms, 2022 This article characterises viral fraction metatranscriptomes (smaller than 0.2 µm) from the pelagic zone of oligotrophic Lake Baikal (Russia). The study revealed the dominance of transcripts of DNA viruses: bacteriophages and algal viruses. We identified transcripts similar to Pithovirus sibericum, a nucleocytoplasmic large DNA virus (NCLDV) isolated ...
Sergey Potapov +5 more
openaire +3 more sources
DNA/RNA Preservation in Glacial Snow and Ice Samples
Frontiers in Microbiology, 2022 The preservation of nucleic acids for high-throughput sequencing is an ongoing challenge for field scientists. In particular, samples that are low biomass, or that have to be collected and preserved in logistically challenging environments (such as ...
Christopher B. Trivedi +10 more
doaj +1 more source
Metatranscriptomic census of active protists in soils [PDF]
The ISME Journal, 2015 Abstract The high numbers and diversity of protists in soil systems have long been presumed, but their true diversity and community composition have remained largely concealed. Traditional cultivation-based methods miss a majority of taxa, whereas molecular barcoding approaches employing PCR introduce significant biases in reported ...
Geisen, S. +6 more
openaire +4 more sources
Periodontology 2000, EarlyView., 2023 Abstract This narrative review summarizes the collective knowledge on periodontal microbiology, through a historical timeline that highlights the European contribution in the global field. The etiological concepts on periodontal disease culminate to the ecological plaque hypothesis and its dysbiosis‐centered interpretation.
Georgios N. Belibasakis +5 more
wiley +1 more source
Transcriptional Shifts Highlight the Role of Nutrients in Harmful Brown Tide Dynamics
Frontiers in Microbiology, 2019 Harmful algal blooms (HABs) threaten ecosystems and human health worldwide. Controlling nitrogen inputs to coastal waters is a common HAB management strategy, as nutrient concentrations often suggest coastal blooms are nitrogen-limited. However, defining
Louie L. Wurch +5 more
doaj +1 more source
Structured RNA Contaminants in Bacterial Ribo-Seq
mSphere, 2020 Ribosome profiling (Ribo-Seq) is a powerful method to study translation in bacteria. However, Ribo-Seq signal can be observed across RNAs that one would not expect to be bound by ribosomes.
Brayon J. Fremin, Ami S. Bhatt
doaj +1 more source
Metatranscriptome profiling of a harmful algal bloom [PDF]
Harmful Algae, 2014 Metagenomic methods provide a powerful means to investigate complex ecological phenomena. Developed originally for study of Bacteria and Archaea, the application of these methods to eukaryotic microorganisms is yet to be fully realized. Most prior environmental molecular studies of eukaryotes have relied heavily on PCR amplification with eukaryote ...
Endymion D, Cooper +4 more
openaire +2 more sources
Frontiers in Microbiology, 2018 While most studies using RNA-stable isotope probing (SIP) to date have focused on ribosomal RNA, the detection of 13C-labeled mRNA has rarely been demonstrated.
Lauren M. Bradford +6 more
doaj +1 more source
Environmental DNA, 2023 Temperate coastal marine waters are often thermally stratified from spring through fall but can be dynamic and disrupted by tidal currents and wind‐driven upwelling. These mixing events introduce deeper, cooler water with a higher partial pressure of CO2
Ben J. G. Sutherland +8 more
doaj +1 more source
Methodologies for probing the metatranscriptome of grassland soil [PDF]
Journal of Microbiological Methods, 2016 Metatranscriptomics provides an opportunity to identify active microbes and expressed genes in complex soil communities in response to particular conditions. Currently, there are a limited number of soil metatranscriptome studies to provide guidance for using this approach in this challenging matrix.
Aaron Garoutte +3 more
openaire +3 more sources