Results 61 to 70 of about 9,285 (257)

Methodologies for probing the metatranscriptome of grassland soil [PDF]

Journal of Microbiological Methods, 2016
Metatranscriptomics provides an opportunity to identify active microbes and expressed genes in complex soil communities in response to particular conditions. Currently, there are a limited number of soil metatranscriptome studies to provide guidance for using this approach in this challenging matrix.
Aaron Garoutte, Erick Cardenas, James Tiedje, Adina Howe   +3 more
openaire   +3 more sources

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Metatranscriptomic Analysis of the Chicken Gut Resistome Response to In-Feed Antibiotics and Natural Feed Additives

Frontiers in Microbiology, 2022
The emergence of resistance against common antibiotics in the gut microbiota is a major issue for both human and livestock health. This highlights the need for understanding the impact of such application on the reservoir of antibiotic resistance genes ...
Raju Koorakula, Raju Koorakula, Matteo Schiavinato, Mahdi Ghanbari, Gertrude Wegl, Nikolaus Grabner, Andreas Koestelbauer, Viviana Klose, Juliane C. Dohm, Konrad J. Domig   +9 more
doaj   +1 more source

High-throughput terrestrial biodiversity assessments: mitochondrial metabarcoding, metagenomics or metatranscriptomics?

, 2018
Consensus on the optimal high-throughput sequencing (HTS) approach to examine biodiversity in mixed terrestrial arthropod samples has not been reached. Metatranscriptomics could increase the proportion of taxonomically informative mitochondrial reads in ...
John-James Wilson, Kong-Wah Sing (489449), Kong-Wah Sing, Han-Ming Gan (3176799), Guo-Jie Brandon-Mong (5038415), Han-Ming Gan, John-James Wilson (489448), Guo-Jie Brandon-Mong   +7 more
core   +1 more source

A Single‐Cell Transcriptomic Atlas of the Ovine Rumen Microbiome Characterizes Lineage‐Specific Metabolic Shifts Associated with Host Heat Tolerance

Advanced Science, EarlyView.
An optimized single‐cell transcriptomic framework profiles over 60 000 cells to map the ovine rumen microbiome, partitioning the ecosystem into seven cross‐species functional clusters. In heat‐resistant hosts, a lineage‐specific metabolic shift in Anaerovibrio lipolyticus toward a highly glycolytic phenotype contributes to a “nutritional sparing ...
Sanbao Zhang, Qinyang Jiang, Junjie Ma, Jianwei Zou, Fan Wang, Feifei Lv, Yanna Huang, Yongcheng Wang, Ziye Xu   +8 more
wiley   +1 more source

Metatranscriptomic Investigation of Adaptation in NO and N2O Production From a Lab-Scale Nitrification Process Upon Repeated Exposure to Anoxic–Aerobic Cycling

Frontiers in Microbiology, 2018
The molecular mechanisms of microbial adaptation to repeated anoxic–aerobic cycling were investigated by integrating whole community gene expression (metatranscriptomics) and physiological responses, including the production of nitric (NO) and nitrous ...
Ariane Coelho Brotto, Medini K Annavajhala, Kartik Chandran   +2 more
doaj   +1 more source

Training Data for "Metatranscriptomics analysis using microbiome RNASeq data"

, 2023
Microbiomes play a critical role in host health, disease, and the environment.. Functional microbiome analysis which estimates the functional groups expressed by microbial community enables researchers to look beyond taxonomic composition and correlation
Saskia Hiltemann, Bérénice Batut, Subina Mehta, Paul Zierep, Praveen Kumar, Pratik Jagtap   +5 more
core   +1 more source

Enhancing Meat Quality Through Dietary Fiber: Insights Into the Gut Microbiota‐Derived Short‐Chain Fatty Acids‐Muscle Axis

Animal Research and One Health, EarlyView.
Dietary fiber (DF) modulates meat quality through the gut‐muscle axis by reshaping gut microbiota, improving intestinal integrity, reducing inflammation, and regulating muscle metabolism. These mechanisms influence muscle fiber characteristics and post‐mortem biochemistry, thereby enhancing tenderness, juiciness, color, flavor, and nutritional value of
Zeshan Zulfiqar, Muhammad Arslan Asif, Layla Al‐Mitib, Nabeela Ashiq, Haojie Wang, Yalei Cui, Boshuai Liu, Shucheng Huang, Yinghua Shi   +8 more
wiley   +1 more source

Bacterial metatranscriptome of dentin caries [PDF]

Journal of Oral Microbiology, 2017
Dental caries results from altered microbial community activity in disease compared to health. Caries advanced into dentin was compared with coronal caries and caries-free sites. Oral bacterial samples were obtained from young children, placed in RNAlater and stored frozen. mRNA was purified and sequenced on an Illumina NextSeq 500 platform.
Anne CR Tanner, Christine Kressirer, Lina Faller, Kristie Lake, Floyd Dewhirst, Alexis Kokarasb, Bruce Paster, Jorge Frias-Lopez   +7 more
openaire   +2 more sources

Metatranscriptomics sample preparation protocol w/ScriptSeq v1

, 2015
This protocol is an update of the Metatranscriptomics protocol from DeLong lab 2012 which incorporates ScriptSeq v2 RNA library preparation. Part A: RNA extraction, DNase treatment, purification, and quantification. Part B: rRNA subtraction Part C: cDNA
Ed DeLong
core   +1 more source