Dual-RNAseq Analysis Unravels Virus-Host Interactions of MetSV and Methanosarcina mazei [PDF]
Methanosarcina spherical virus (MetSV), infecting Methanosarcina species, encodes 22 genes, but their role in the infection process in combination with host genes has remained unknown.
Finn O. Gehlert +6 more
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Small protein mediates inhibition of ammonium transport in Methanosarcina mazei—an ancient mechanism? [PDF]
In the past decade, small open reading frames (sORFs) coding for proteins less than 70 amino acids (aa) in length have moved into the focus of science. sORFs and the corresponding small proteins have been recently identified in all three domains of life.
Tim Habenicht +5 more
doaj +2 more sources
Cometabolism of ferrihydrite reduction and methyl-dismutating methanogenesis by Methanosarcina mazei [PDF]
Recent discoveries have shown that some Methanosarcina species can reduce Fe(III), reshaping our understanding of Methanosarcina ecophysiology. However, the specific minerals reduced, the products formed, and the underlying metabolic mechanisms remain ...
Chaojie Guo, Yahai Lu
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Mining Proteomic Data to Expose Protein Modifications in Methanosarcina mazei strain Gö1 [PDF]
Proteomic tools identify constituents of complex mixtures, often delivering long lists of identified proteins. The high-throughput methods excel at matching tandem mass spectrometry data to spectra predicted from sequence databases.
Deborah eLeon +6 more
doaj +2 more sources
Uncovering the small proteome of Methanosarcina mazei using Ribo-seq and peptidomics under different nitrogen conditions [PDF]
The mesophilic methanogenic archaeal model organism Methanosarcina mazei strain Gö1 is crucial for climate and environmental research due to its ability to produce methane. Here, we establish a Ribo-seq protocol for M.
Muhammad Aammar Tufail +11 more
doaj +2 more sources
2-oxoglutarate triggers assembly of active dodecameric Methanosarcina mazei glutamine synthetase [PDF]
Glutamine synthetases (GS) are central enzymes essential for the nitrogen metabolism across all domains of life. Consequently, they have been extensively studied for more than half a century.
Eva Herdering +7 more
doaj +2 more sources
Co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron(III)-reducing enrichment culture [PDF]
Methanosaeta harundinacea and Methanosarcina barkeri, known as classic acetoclastic methanogens, are capable of directly accepting electrons from Geobacter metallireducens for the reduction of carbon dioxide to methane, having been revealed as direct ...
Shiling eZheng +8 more
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Isolation and characterization of new strains KOR-3, -4, -5, and -6 from an anaerobic digester using pig slurry [PDF]
Objective An experiment was conducted to isolate and identify new methanogens in Korea from an anaerobic digester that uses pig slurry. Methods An anaerobic digestate sample was collected from an anaerobic digester using pig slurry.
Urantulkhuur Battumur +3 more
doaj +2 more sources
The CARF Protein MM_0565 Affects Transcription of the Casposon-Encoded cas1-solo Gene in Methanosarcina mazei Gö1 [PDF]
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci are found in bacterial and archaeal genomes where they provide the molecular machinery for acquisition of immunity against foreign DNA.
Andrea Ulbricht +6 more
doaj +2 more sources
Carbon-dependent control of electron transfer and central carbon pathway genes for methane biosynthesis in the Archaean,
Background The archaeon, Methanosarcina acetivorans strain C2A forms methane, a potent greenhouse gas, from a variety of one-carbon substrates and acetate. Whereas the biochemical pathways leading to methane formation are well understood, little is known
Gunsalus Robert P, Rohlin Lars
doaj +4 more sources

