, 2016 The outermost cell envelope structure of many archaea and bacteria contains a proteinaceous lattice termed the surface layer or S-layer. It is typically composed of only one or two abundant, often post-translationally modified proteins that self-assemble
Robert P. Gunsalus (2382934) +9 more
core +26 more sources
The CARF Protein MM_0565 Affects Transcription of the Casposon-Encoded cas1-solo Gene in Methanosarcina mazei Gö1 [PDF]
Biomolecules, 2020 Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) loci are found in bacterial and archaeal genomes where they provide the molecular machinery for acquisition of immunity against foreign DNA.
Andrea Ulbricht +6 more
doaj +2 more sources
Structure of the Methanosarcina mazei Mtr complex bound to the oxygen-stress responsive small protein MtrI [PDF]
Nature CommunicationsMethanogenic archaea emit ~1 Gt of methane annually, impacting global carbon cycling and climate. Central to their energy metabolism is a membrane-bound, sodium-translocating methyltransferase complex: the N⁵-tetrahydromethanopterin:CoM-S ...
Tristan Reif-Trauttmansdorff +8 more
doaj +2 more sources
The H+-Translocating Inorganic Pyrophosphatase From Arabidopsis thaliana Is More Sensitive to Sodium Than Its Na+-Translocating Counterpart From Methanosarcina mazei [PDF]
Frontiers in Plant Science, 2020 Overexpression of membrane-bound K+-dependent H+-translocating inorganic pyrophosphatases (H+-PPases) from higher plants has been widely used to alleviate the sensitivity toward NaCl in these organisms, a strategy that had been previously tested in ...
José R. Pérez-Castiñeira +1 more
doaj +2 more sources
Cold Orthogonal Translation: A Psychrophilic Pyrrolysyl-tRNA Synthetase Boosts Genetic Code Expansion in E. coli. [PDF]
Adv Sci (Weinh)ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Koch NG +4 more
europepmc +2 more sources
Advanced ScienceHigh total ammonia nitrogen (TAN) inhibits anaerobic digestion (AD) and cannot be completely eliminated by merely enhancing a stage of AD. This study incorporates TAN‐tolerant inoculum into substrates hydrolyzed by Rhizopus mixed agents to simultaneously
Heng Wu +6 more
doaj +2 more sources
Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives [PDF]
Frontiers in Molecular BiosciencesIntroductionPyrrolysyl-tRNA synthetase (PylRS) is a key enzyme for the site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. However, its native form has a limited substrate scope.
Anastasia Dakhnevich +4 more
doaj +2 more sources
Cross-cleavage activity of Cas6b in crRNA processing of two different CRISPR-Cas systems in Methanosarcina mazei Gö1 [PDF]
RNA Biology, 2019 Omer S Alkhnbashi +2 more
exaly +2 more sources
Putative Extracellular Electron Transfer in Methanogenic Archaea
Frontiers in Microbiology, 2021 It has been suggested that a few methanogens are capable of extracellular electron transfers. For instance, Methanosarcina barkeri can directly capture electrons from the coexisting microbial cells of other species.
Kailin Gao, Yahai Lu
doaj +1 more source
Toxicity of long chain fatty acids towards acetate conversion by Methanosaeta concilii and Methanosarcina mazei. [PDF]
Microb Biotechnol, 2016 Long-chain fatty acids (LCFA) can inhibit methane production by methanogenic archaea. The effect of oleate and palmitate on pure cultures of Methanosaeta concilii and Methanosarcina mazei was assessed by comparing methane 11% and 64% methanogenic ...
Silva SA +6 more
europepmc +2 more sources