Results 11 to 20 of about 4,218 (198)

Structure of the Methanosarcina mazei Mtr complex bound to the oxygen-stress responsive small protein MtrI [PDF]

Nature Communications
Methanogenic archaea emit ~1 Gt of methane annually, impacting global carbon cycling and climate. Central to their energy metabolism is a membrane-bound, sodium-translocating methyltransferase complex: the N⁵-tetrahydromethanopterin:CoM-S ...
Tristan Reif-Trauttmansdorff, Eva Herdering, Stefan Bohn, Tomas Pascoa, Jörg Kahnt, Erik Zimmer, Anuj Kumar, Ruth A. Schmitz, Jan M. Schuller   +8 more
doaj   +2 more sources

The H+-Translocating Inorganic Pyrophosphatase From Arabidopsis thaliana Is More Sensitive to Sodium Than Its Na+-Translocating Counterpart From Methanosarcina mazei [PDF]

Frontiers in Plant Science, 2020
Overexpression of membrane-bound K+-dependent H+-translocating inorganic pyrophosphatases (H+-PPases) from higher plants has been widely used to alleviate the sensitivity toward NaCl in these organisms, a strategy that had been previously tested in ...
José R. Pérez-Castiñeira, Aurelio Serrano   +1 more
doaj   +2 more sources

Toxicity of long chain fatty acids towards acetate conversion by Methanosaeta concilii and Methanosarcina mazei. [PDF]

Microb Biotechnol, 2016
Long-chain fatty acids (LCFA) can inhibit methane production by methanogenic archaea. The effect of oleate and palmitate on pure cultures of Methanosaeta concilii and Methanosarcina mazei was assessed by comparing methane production rates from acetate ...
Silva SA, Salvador AF, Cavaleiro AJ, Pereira MA, Stams AJ, Alves MM, Sousa DZ.   +6 more
europepmc   +4 more sources

Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives [PDF]

Frontiers in Molecular Biosciences
IntroductionPyrrolysyl-tRNA synthetase (PylRS) is a key enzyme for the site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. However, its native form has a limited substrate scope.
Anastasia Dakhnevich, Sabina Miasoutova, Danila Iliushin, Aleksey Rozanov, Roman Ivanov   +4 more
doaj   +2 more sources

Overcoming Extreme Ammonia Inhibition on Methanogenesis by Artificially Constructing a Synergistically Community with Acidogenic Bacteria and Hydrogenotrophic Archaea

Advanced Science
High total ammonia nitrogen (TAN) inhibits anaerobic digestion (AD) and cannot be completely eliminated by merely enhancing a stage of AD. This study incorporates TAN‐tolerant inoculum into substrates hydrolyzed by Rhizopus mixed agents to simultaneously
Heng Wu, Huaiwen Zhang, Taili Dong, Zhenyu Li, Xiaohui Guo, Heyu Chen, Yiqing Yao   +6 more
doaj   +2 more sources

Functional analysis of archaeal MBF1 by complementation studies in yeast [PDF]

Biology Direct, 2011
Background Multiprotein-bridging factor 1 (MBF1) is a transcriptional co-activator that bridges a sequence-specific activator (basic-leucine zipper (bZIP) like proteins (e.g. Gcn4 in yeast) or steroid/nuclear-hormone receptor family (e.g.
Siebers Bettina, Pons Tirso, Ehrenhofer-Murray Ann E, Marrero Coto Jeannette   +3 more
doaj   +5 more sources

An innovative strategy for overcoming ultra-high ammonia nitrogen inhibition on anaerobic methanogenesis via stepwise domestication [PDF]

Microbiome
Background The world is facing both an increasingly severe energy crisis and a growing problem of agricultural pollution. The utilization of agricultural waste by anaerobic digestion (AD), has received increasing attention.
Heng Wu, Taili Dong, Anjie Li, Jing Chen, Huaiwen Zhang, Hongyi Lv, Caiyun Yang, Xiaohui Guo, Xuanmin Yang, Ling Qiu, Changlin Miao, Yiqing Yao   +11 more
doaj   +2 more sources

Active in vivo translocation of the Methanosarcina mazei Gö1 Casposon. [PDF]

Nucleic Acids Res, 2023
Abstract Casposons are transposable elements containing the CRISPR associated gene Cas1solo. Identified in many archaeal genomes, casposons are discussed as the origin of CRISPR-Cas systems due to their proposed Cas1solo-dependent translocation.
Gehlert FO, Nickel L, Vakirlis N, Hammerschmidt K, Vargas Gebauer HI, Kießling C, Kupczok A, Schmitz RA.   +7 more
europepmc   +3 more sources

Functional insights into Solo-Cas4 in <i>Methanosarcina mazei</i> Gö1. [PDF]

Microlife
Abstract Solo-Cas4 homologs are Cas4-family proteins found outside of canonical CRISPR–Cas operons. Here, we present the biochemical characterization of Solo-Cas4 from Methanosarcina mazei Gö1. We found significantly upregulated solo-cas4 transcript levels during stationary phase, while remaining constant under oxygen exposure ...
Rentz L, Hellwig L, Schneider S, Schmitz RA.   +3 more
europepmc   +3 more sources

Putative Extracellular Electron Transfer in Methanogenic Archaea

Frontiers in Microbiology, 2021
It has been suggested that a few methanogens are capable of extracellular electron transfers. For instance, Methanosarcina barkeri can directly capture electrons from the coexisting microbial cells of other species.
Kailin Gao, Yahai Lu
doaj   +1 more source