Ultrastructure of Methanosarcina mazei
Summary Thin and semi-thin sections and freeze fracture replicas of unfixed, propane jet frozen cells of the methanogenic bacterium Methanosarcina mazei were examined in normal and medium-voltage (200 KV) transmission electron microscopes. A typical unit membrane surrounds the cytoplasm.
Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611, U.S.A. ( host institution ) +3 more
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Differential regulation of Ota and Otb, two primary glycine betaine transporters in the methanogenic archaeon Methanosarcina mazei go1 [PDF]
Methanogenic archaea accumulate glycine betaine in response to hypersalinity, but the regulation of proteins involved, their mechanism of activation and regulation of the corresponding genes are largely unknown.
Hoffmann, M. +5 more
core +1 more source
Resonance Raman analysis of intracellular vitamin B12 analogs in methanogenic archaea
Methanogenic archaea (methanogens) are microorganisms that can synthesize methane. They are found in diverse environments ranging from paddy fields to animal digestive tracts to deep‐sea hydrothermal vents.
Nanako Kanno +4 more
doaj +1 more source
Phylogenetic differences in content and intensity of periodic proteins [PDF]
Many proteins exhibit sequence periodicity, often correlated with a visible structural periodicity. The statistical significance of such periodicity can be assessed by means of a chi-square-based test, with significance thresholds being calculated from ...
Gatherer, D., McEwan, N.R.
core +1 more source
Characterization of Methanosarcina barkeri MST and 227, Methanosarcina mazei S-6T, and Methanosarcina vacuolata Z-761T [PDF]
Members of the genus Methanosarcina are recognized as major aceticlastic methanogens, and several species which thrive in low-salt, pH-neutral culture medium at mesophilic temperatures have been described. However, the environmental conditions which support the fastest growth of these species (Methanosarcina barkeri MST [T = type strain] and 227 ...
G. M. MAESTROJUAN, D. R. BOONE
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Selenocysteine, pyrrolysine and the unique energy metabolism of methanogenic archaea [PDF]
Methanogenic archaea are a group of strictly anaerobic microorganisms characterized by their strict dependence on the process of methanogenesis for energy conservation.
Rother, Michael, Krzycki, Joseph A.
core +1 more source
Insights into the NrpR regulon in Methanosarcina mazei Gö1
The methanogenic archaeon Methanosarcina mazei strain Gö1 contains two homologues of NrpR, the transcriptional repressor of nitrogen assimilation genes recently discovered and characterized in Methanococcus maripaludis. Insertion of a puromycin-resistance conferring cassette into MM1085 encoding a single NrpR domain with an N-terminal helix-turn-helix ...
Weidenbach, Katrin +4 more
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Immunochemistry and localization of the enzyme disaggregatase in Methanosarcina mazei [PDF]
The enzyme disaggregatase (Dag) from Methanosarcina mazei was studied immunochemically. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of purified Dag under reducing and nonreducing conditions revealed a single band with a 94-kDa molecular mass.
E, Conway de Macario +3 more
openaire +2 more sources
Rational design of the genetic code expansion toolkit for in vivo encoding of D-amino acids
Once thought to be non-naturally occurring, D-amino acids (DAAs) have in recent years been revealed to play a wide range of physiological roles across the tree of life, including in human systems.
Han-Kai Jiang +15 more
doaj +1 more source
ThermoPhyl : a software tool for selecting phylogenetically optimized conventional and quantitative-PCR taxon-targeted assays for use with complex samples [PDF]
The ability to specifically and sensitively target genotypes of interest is critical for the success of many PCR-based analyses of environmental or clinical samples that contain multiple templates.Next-generation sequence data clearly show that such ...
Dowd, Scot E. +2 more
core +1 more source

