Characterization of a feedback-resistant mevalonate kinase from the archaeon Methanosarcina mazei. [PDF]
ABSTRACT The mevalonate pathway is utilized for the biosynthesis of isoprenoids in many bacterial, eukaryotic, and archaeal organisms. Based on previous reports of its feedback inhibition, mevalonate kinase (MVK) may play an important regulatory role in the biosynthesis of mevalonate pathway-derived compounds.
Primak YA +6 more
europepmc +4 more sources
Involvement of Ech hydrogenase in energy conservation of Methanosarcina mazei [PDF]
Methanosarcina mazei belongs to the group of aceticlastic methanogens and converts acetate into the potent greenhouse gases CO 2 and CH 4 .
Cornelia, Welte +2 more
openaire +2 more sources
This study reports the application of expanding genetic codes in developing protein cage-based delivery systems. The evolved Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)•tRNAPyl pairs derived from directed evolution are examined to probe their
Yi-Hui Wang +9 more
doaj +1 more source
Analyzing conformational changes in single FRET-labeled A1 parts of archaeal A1AO-ATP synthase
ATP synthases utilize a proton motive force to synthesize ATP. In reverse, these membrane-embedded enzymes can also hydrolyze ATP to pump protons over the membrane.
Börsch, Michael +3 more
core +1 more source
S-layer, Surface-Accessible, and Concanavalin A Binding Proteins of Methanosarcina acetivorans and Methanosarcina mazei [PDF]
The outermost cell envelope structure of many archaea and bacteria contains a proteinaceous lattice termed the surface layer or S-layer. It is typically composed of only one or two abundant, often posttranslationally modified proteins that self-assemble to form the highly organized arrays.
Deborah R, Francoleon +9 more
openaire +2 more sources
Physiological Evidence for Isopotential Tunneling in the Electron Transport Chain of Methane-Producing Archaea [PDF]
Many, but not all, organisms use quinones to conserve energy in their electron transport chains. Fermentative bacteria and methane-producing archaea (methanogens) do not produce quinones but have devised other ways to generate ATP. Methanophenazine (MPh)
Buan, Nicole R., Duszenko, Nikolas
core +1 more source
SICLE: A high-throughput tool for extracting evolutionary relationships from phylogenetic trees
We present the phylogeny analysis software SICLE (Sister Clade Extractor), an easy-to-use, high- throughput tool to describe the nearest neighbors to a node of interest in a phylogenetic tree as well as the support value for the relationship.
DeBlasio, Dan, Wiscaver, Jennifer
core +2 more sources
Small protein MtrR is a regulator of the Mtr methyltransferase complex in Methanosarcina mazei. It binds specifically to the MtrA subunit and modulates Mtr activity in response to hydrogen (H2) availability. Deleting mtrR impairs growth in the presence but not absence of H2, indicating its role in directing methyl transfer toward an energy‐conserving ...
Tim Habenicht +6 more
wiley +1 more source
Lamina, a novel multicellular form of Methanosarcina mazei S-6
A novel multicellular form of Methanosarcina mazei S-6 is described. It was termed lamina, and it formed during the exponential growth phase when packets or single cells were grown in 40 mM trimethylamine and a total concentration of 8.3 to 15.6 mM Ca2+ and/or Mg2+, in cultures that were not shaken.
L E, Mayerhofer +2 more
openaire +3 more sources
Anaerobic microbial LCFA degradation in bioreactors [PDF]
This paper reviews recent results obtained on long-chain fatty acids (LCFA) anaerobic degradation. Two LCFA were used as model substrates: oleate, a mono-unsaturated LCFA, and palmitate, a saturated LCFA, both abundant in LCFA-rich wastewaters.
Alves, J. I. +5 more
core +2 more sources

