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Positional specificity of microbial lipases
Journal of Fermentation and Bioengineering, 1991The positional specificity of microbial lipases was studied by a method based on the measurement of diglyceride (DG) isomers in their reaction products using a TLC-autodetector and calculation of a positional specificity index defined as follows: PSI = [1, 2(2, 3)DG − 1, 3DG × 2] × 100/[1, 2(2,3)DG + 1,3DG × 2].
Miwa Matori +2 more
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Overproduction of microbial lipids and lipases
Folia Microbiologica, 1991The nomenclature of simple and complex lipids, and their analysis are introduced and the overproduction of lipids and lipases are discussed.
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Recombinant microbial lipases for biotechnological applications
Bioorganic & Medicinal Chemistry, 1999Lipases, mainly of microbial origin, represent the most widely used class of enzymes in biotechnological applications and organic chemistry. Modern methods of genetic engineering combined with an increasing knowledge of structure and function will allow further adaptation to industrial needs and exploration of novel applications.
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Industrial application of microbial lipases: A review
Journal of the American Oil Chemists' Society, 1974AbstractCommercial microbial lipases have been used in dairy and other food processes, and lipases produced in situ by microorganisms are important in making foods palatable and acceptable. Microbial lipases have been used in detergents, pharmaceuticals, cosmetics, leather processing, production of aliphatic acids, and in the treatment of domestic and ...
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Microbial Lipases and Their Versatile Applications
2020Lipases are important biocatalysts and known for their versatile biotechnological applications. Lipases are well known as triacylglycerol hydrolases converting fats into simpler compounds as glycerol and fatty acids at the water-lipid interface and vice versa in nonaqueous medium.
Neha Vishnoi +2 more
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Production and optimization of microbial lipase
Bioprocess Engineering, 1998Production of lipase by the newly isolated Pseu-domonas species has been optimised. Various parameters like initial pH, temperature, incubation period, effect of agitation, inoculum age, inoculum concentration were optimised. It was observed that modified GYP media with 72 hrs incubation, pH 5.5, at 37 °C in agitation conditions were optimum for growth
Sarada Sarkar +4 more
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Glyceride synthesis by four kinds of microbial lipase
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977Apart from their usual mechanism of action, lipases from Aspergillus niger and Rhizopus delemar also catalyzed the synthesis of glycerides from oleic acid and glycerol. Lipases from Geotrichum candidum and Penicillium cyclopium were inactivated by oleic acid, but were stable in the presence of casein, albumin or buffer of appropriate pH.
Y, Tsujisaka, S, Okumura, M, Iwai
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[The characterization of microbial lipases. 2. The determination of lipase specificity].
Die Nahrung, 1991Types of lipase specificity are as follows: Positional specificity; fatty acid specificity; stereospecificity; substrate specificity (different rates of lipolysis of different glyceride classes. The acylglycerol used for determination of lipase specificity must be so structured, that specificities are not confused and unambiguous results are obtained ...
M, Bariszlovich, D, Meusel, M, Tülsner
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[The characterization of microbial lipases. 1. The determination of lipase activity].
Die Nahrung, 1990In the selection of an appropriate method for activity determination of lipases existing technical equipment, kind of enzymes, number of samples investigated (e.g. in routine analysis), and expected sensitivity range have to be taken into account. Titrimetric methods and above all copper salt methods with their high detection sensitivity are the most ...
M, Bariszlovich, D, Meusel, M, Tülsner
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