Results 171 to 180 of about 107,595 (212)

Electrochemical and conformational studies of microcystin–LR

Analytica Chimica Acta, 2000
Abstract The detection of microcystins in freshwater and in biological samples has become increasingly important due to their toxicity and tumor-promoting nature. This paper describes the electrochemical characterization of microcystin–LR using cyclic voltammetry and anodic stripping voltammetry at different substrates of gold, platinum, glassy ...
Yan, F, Ozsoz, M, Sadik, OA
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A Fluorescent Microplate Assay for Microcystin-LR

Analytical Biochemistry, 1999
A fluorescent enzyme inhibition assay for microcystin-LR was developed using a new fluorescent substrate of protein phosphatases 1 (PP1) and 2A (PP2A), 6,8-difluoro-4-methylumbelliferyl phosphate. The PP1 and PP2A inhibition assay for microcystin-LR was performed in a microtiter plate and the fluorescence yielded by the enzymatic hydrolysis of the ...
O I, Fontal, M R, Vieytes, J M, Baptista de Sousa, M C, Louzao, L M, Botana   +4 more
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The influence of microcystin-LR on fish phagocytic cells

Human & Experimental Toxicology, 2007
The in vitro influence of microcystin-LR (MC-LR) on rainbow trout phagocytic cell functions was studied. The cells isolated from the fish blood were exposed on MC-LR at the concentrations of 1, 5, 10, 20 μg/mL. The viability of the cells after 2, 4 and 24 hour incubation with MC-LR was studied as well as phagocytic cell ability measured as zymozan ...
A, Sierosławska, A, Rymuszka, A, Bownik, T, Skowroński   +3 more
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Study on the cytotoxicity of microcystin-LR on cultured cells

Chemosphere, 2000
The toxicity of purified blue-green algal toxin, microcystin-LR, on permanent cell lines KB, NIH/3T3, H-4-II-E, HeLa, Vero, Hep G2, Caco-2 and HL-60 was studied. Assessment of cell viability using colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays indicated that purified microcystin-LR induced toxic effect on KB and ...
M W, Chong, K D, Gu, P K, Lam, M, Yang, W F, Fong   +4 more
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Microcystin-LR-induced Ultrastructural Changes in Rats

Veterinary Pathology, 1990
The ultrastructure of hepatic, pulmonary, and renal lesions was evaluated in rats injected intraperitoneally with a lethal dose of microcystin-LR (MCLR, 160 μg/kg), a cyclic heptapeptide hepatotoxin produced by the blue-green algae, Microcystis aeruginosa.
S B, Hooser, V R, Beasley, E J, Basgall, W W, Carmichael, W M, Haschek   +4 more
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Exploring the interaction of microcystin-LR with proteins and DNA

Toxicology in Vitro, 2008
The physiological role of microcystin-LR is still under discussion, and since binding of microcystin-LR to proteins different from their main cellular targets was described, we have performed experiments in order to explore this interaction. A non-specific interaction of microcystin-LR with a variety of soluble proteins in vitro is disrupted when using
L, Vela, E, Sevilla, C, Gonzalez, M T, Bes, M F, Fillat, M L, Peleato   +5 more
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Brönsted Catalyzed Hydrolysis of Microcystin-LR by Siderite

Environmental Science & Technology, 2018
Six naturally occurring minerals were employed to catalyze the hydrolysis of microcystin-LR (MC-LR) in water. After preliminary screening experiments, siderite stood out among these minerals due to its higher activity and selectivity. In comparison with kaolinite, which is known to act as a Lewis acid catalyst, siderite was found to act primarily as a ...
Yanfen Fang, Wei Zhou, Changcun Tang, Yingping Huang, David Mark Johnson, Zhiyong Jason Ren, Wanhong Ma   +6 more
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Microcystin‐LR in Brazilian Aquaculture Production Systems

Water Environment Research, 2010
The growth of aquaculture production systems, mostly the sport‐fishing kind, coupled with a lack of control, brings about concerns on the quality of water and food produced. The current paper determines which factors may trigger the growth of cyanobacteria, with subsequent concentrations of microcystins in collected water samples, at the surface and in
Barros, Ludmilla S. S., de Souza, Fagner Correia, Tavares, Lucia Helena S., Amaral, Luiz A.   +3 more
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Comprehensive study of proteins that interact with microcystin-LR

Analytical and Bioanalytical Chemistry, 2011
We carried out a comprehensive study of proteins that exhibit specific interactions with a naturally occurring toxin, microcystin (MC)-LR, in order to gain insight into the unknown underlying mechanism of MC virulence. This audacious study employed a simple affinity test that used MC-LR immobilized on an original ethylene oxide based monolithic solid ...
Tomoko, Mori, Takuya, Kubo, Kunimitsu, Kaya, Ken, Hosoya   +3 more
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Hepatotoxicity of microcystin-LR in fed and fasted rats

Toxicon, 1991
The LD50 (25 hr, i.p.) for microcystin-LR in fed rats (122 micrograms/kg) was significantly higher than that in fasted rats (72 micrograms/kg). At doses of 100, 150 and 200 micrograms of microcystin-LR per kg, the median times to death were 31.9, 18.2 and 11.2 hr for fed rats, and 1.8, 1.7 and 1.5 hr for fasted rats. A sublethal dose of microcystin (50
G A, Miura, N A, Robinson, W B, Lawrence, J G, Pace   +3 more
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