Results 181 to 190 of about 11,325 (225)

A Fluorescent Microplate Assay for Microcystin-LR

Analytical Biochemistry, 1999
A fluorescent enzyme inhibition assay for microcystin-LR was developed using a new fluorescent substrate of protein phosphatases 1 (PP1) and 2A (PP2A), 6,8-difluoro-4-methylumbelliferyl phosphate. The PP1 and PP2A inhibition assay for microcystin-LR was performed in a microtiter plate and the fluorescence yielded by the enzymatic hydrolysis of the ...
O I, Fontal, M R, Vieytes, J M, Baptista de Sousa, M C, Louzao, L M, Botana   +4 more
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Effects of microcystin-LR on mouse lungs

Toxicon, 2007
Toxic cyanobacteria blooms in drinking water supplies have been an increasing public health concern all over the world. Human populations can be exposed to microcystins, an important family of cyanotoxins, mainly by oral ingestion. However, inhalation from recreational water and hemodialysis can represent other routes.
Raquel M, Soares, Viviane R, Cagido, Rodrigo B, Ferraro, José Roberto, Meyer-Fernandes, Patrícia R M, Rocco, Walter A, Zin, Sandra M F O, Azevedo   +6 more
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Rapid chemiluminescence biosensing of microcystin-LR

Analytica Chimica Acta, 2009
A rapid immunoassay for sensitive detection of microcystin-LR using a portable chemiluminescence multichannel immunosensor (CL-MADAG) was developed. The sensor device is based on a capillary ELISA technique in combination with a miniaturized fluidics system and uses chemiluminescence as the detection principle.
Petra, Lindner, Ramona, Molz, Erwin, Yacoub-George, Hans, Wolf   +3 more
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Investigations into the biodegradation of microcystin-LR in wastewaters

Journal of Hazardous Materials, 2010
Microcystins are potent hepatotoxins that can be produced by cyanobacteria. These organisms can proliferate in wastewaters due to a number of factors including high concentrations of nutrients for growth. As treated wastewaters are now being considered as supplementary drinking water sources, in addition to their frequent use for irrigated agriculture,
Ho, L., Hoefel, D., Palazot, S., Sawade, E., Newcombe, G., Saint, C., Brookes, J.   +6 more
openaire   +4 more sources

The influence of microcystin-LR on fish phagocytic cells

Human & Experimental Toxicology, 2007
The in vitro influence of microcystin-LR (MC-LR) on rainbow trout phagocytic cell functions was studied. The cells isolated from the fish blood were exposed on MC-LR at the concentrations of 1, 5, 10, 20 μg/mL. The viability of the cells after 2, 4 and 24 hour incubation with MC-LR was studied as well as phagocytic cell ability measured as zymozan ...
A, Sierosławska, A, Rymuszka, A, Bownik, T, Skowroński   +3 more
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Comprehensive study of proteins that interact with microcystin-LR

Analytical and Bioanalytical Chemistry, 2011
We carried out a comprehensive study of proteins that exhibit specific interactions with a naturally occurring toxin, microcystin (MC)-LR, in order to gain insight into the unknown underlying mechanism of MC virulence. This audacious study employed a simple affinity test that used MC-LR immobilized on an original ethylene oxide based monolithic solid ...
Tomoko, Mori, Takuya, Kubo, Kunimitsu, Kaya, Ken, Hosoya   +3 more
openaire   +2 more sources

Study on the cytotoxicity of microcystin-LR on cultured cells

Chemosphere, 2000
The toxicity of purified blue-green algal toxin, microcystin-LR, on permanent cell lines KB, NIH/3T3, H-4-II-E, HeLa, Vero, Hep G2, Caco-2 and HL-60 was studied. Assessment of cell viability using colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays indicated that purified microcystin-LR induced toxic effect on KB and ...
M W, Chong, K D, Gu, P K, Lam, M, Yang, W F, Fong   +4 more
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Microcystin-LR-induced Ultrastructural Changes in Rats

Veterinary Pathology, 1990
The ultrastructure of hepatic, pulmonary, and renal lesions was evaluated in rats injected intraperitoneally with a lethal dose of microcystin-LR (MCLR, 160 μg/kg), a cyclic heptapeptide hepatotoxin produced by the blue-green algae, Microcystis aeruginosa.
S B, Hooser, V R, Beasley, E J, Basgall, W W, Carmichael, W M, Haschek   +4 more
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Exploring the interaction of microcystin-LR with proteins and DNA

Toxicology in Vitro, 2008
The physiological role of microcystin-LR is still under discussion, and since binding of microcystin-LR to proteins different from their main cellular targets was described, we have performed experiments in order to explore this interaction. A non-specific interaction of microcystin-LR with a variety of soluble proteins in vitro is disrupted when using
L, Vela, E, Sevilla, C, Gonzalez, M T, Bes, M F, Fillat, M L, Peleato   +5 more
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The adsorption of microcystin-LR by natural clay particles

Toxicon, 2000
The microcystin cyanobacterial hepatotoxins represent an increasingly severe global health hazard. Since microcystins are found world wide in drinking water reservoirs concern about the impact on human health has prompted investigations into remedial water treatment methods.
R J, Morri, D E, Williams, H A, Luu, C F, Holmes, R J, Andersen, S E, Calvert   +5 more
openaire   +2 more sources