Results 81 to 90 of about 47,561 (246)

A guide to laser microdissection‐based proteomics

The FEBS Journal, EarlyView.
This review takes researchers through the full laser microdissection proteomics workflow—from tissue preparation and image analysis to sample processing and mass spectrometry—offering practical solutions to common challenges at each step. Real‐world examples from across the field illustrate how the technology is being applied to disease tissue, making ...
Cheng Shen, Kael Schoffer, Charlotte Williams, Maria C. Tanzer   +3 more
wiley   +1 more source

Mitochondrial respiratory capacities are not linked to individual variation in hypoxia tolerance and standard metabolic rate in European seabass Dicentrarchus labrax

Journal of Fish Biology, EarlyView.
Abstract In hypoxic environments, fish attempt to regulate their metabolic rate as oxygen levels in the water decline. Individuals that have low energy requirements for maintenance – standard metabolic rate (SMR) – are expected to have higher hypoxia tolerance, measured as the critical water oxygen saturation threshold (Scrit) below which they cannot ...
Elisa Thoral, Julie Nati, Jean‐Baptiste Quémeneur, Léann Lozac'h, Germain Salou, Alain Vergnet, Mathieu Besson, Marc Vandeputte, François Allal, Karine Salin, David J. McKenzie, Loïc Teulier   +11 more
wiley   +1 more source

A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis

Plant Methods, 2019
Background Gene expression changes that govern essential biological processes can occur at the cell-specific level. To gain insight into such events, laser microdissection is applied to cut out specific cells or tissues from which RNA for gene expression
Stian Olsen, Kirsten Krause
doaj   +1 more source

Melanoma and Other Melanistic Lesions in Brown Bullhead Ameiurus nebulosus From Waterbodies in the Northeastern United States and Canada: Identification of Risk Factors

Journal of Fish Diseases, EarlyView.
ABSTRACT Melanistic lesions, including non‐raised black areas due to proliferations of melanocytes and melanomacrophages in the dermis and epidermis, as well as raised black areas consistent with melanoma, are described in brown bullhead (BBH) Ameiurus nebulosus from three water bodies in the northeastern United States and Quebec, Canada.
Vicki S. Blazer, Peter Emerson, Matthew Bodnar, Thomas Jones, David R. Russell, Matthew Pehrson, Cheyenne R. Smith, Danielle Cleveland, Mark J. Henderson, Patricia M. Mazik   +9 more
wiley   +1 more source

Salt glands in exo‐recretohalophytes: Development, physiological functions, and prospects for improving crop salt tolerance

Journal of Integrative Plant Biology, EarlyView.
This review examines salt glands in exo‐recretohalophytes, in which epidermal stem cells differentiate into unicellular, bicellular, or multicellular salt glands. Salt ions are transported to the leaves via the transpiration stream and enter salt glands through symplastic and apoplastic pathways. Finally, salt glands actively secrete salt ions from the
Limin Wang, Junyan Xie, Yiping Zou, Chunliang Yao, Hai Fan, Chenqi Shen, Wenyan Zhou, Jingran Qin, Xinke Zhang, Baoshan Wang, Jian Zhang, Guoliang Han   +11 more
wiley   +1 more source

Laser capture microdissection: from its principle to applications in research on neurodegeneration

Neural Regeneration Research, 2015
History and the principle of laser capture microdissection (LCM): LCM, also known as laser microdissection and pressure catapulting, is one of the most powerful and useful techniques in various research areas where isolation of heterogeneous cell ...
S. Chung, Weiyong Shen
semanticscholar   +1 more source

Bioimage analysis in deep visual proteomics: Advancing transparency, reproducibility, and FAIR principles

Journal of Microscopy, EarlyView.
Abstract Deep Visual Proteomics (DVP) combines high‐resolution microscopy, computational instance segmentation, laser capture microdissection (LMD), and ultrasensitive mass spectrometry to enable spatially resolved molecular analysis of defined cellular populations.
Devon Siemes, Angelo Novak, Stephanie Thiebes, Lars Widera, Stephanie Tautges, Hannah Voss, Lars Borgards, Dominik Habermann, Jianxu Chen, Olga Shevchuk, Daniel R. Engel   +10 more
wiley   +1 more source

Digitally Guided Microdissection Aids Somatic Mutation Detection in Difficult to Dissect Tumors

Cancer Genetics, 2015
Molecular genetic testing on formalin fixed, paraffin embedded (FFPE) tumors frequently requires dissection of tumor from tissue sections mounted on glass slides.
K. Geiersbach, N. Adey, Noah C. Welker, D. Elsberry, E. Malmberg, Sumie Edwards, E. Downs‐Kelly, Mohamed Salama, M. Bronner   +8 more
semanticscholar   +1 more source

The cochlear morphometry compendium: High‐resolution synchrotron measurements and normative reference values

Journal of Anatomy, EarlyView.
In this study, 100 cadaveric temporal bones underwent synchrotron radiation phase‐contrast imaging. Images were segmented to produce 3D surface mesh models for morphological analysis. Measurements obtained for all cochleae included: standard gross dimensions, various cochlear lengths including basilar membrane length, detailed intracochlear scalar ...
Sumit K. Agrawal, Hao Li, Carmine Spedaliere, Helge Rask‐Andersen, Hanif M. Ladak   +4 more
wiley   +1 more source

SIVQ-aided laser capture microdissection: A tool for high-throughput expression profiling

Journal of Pathology Informatics, 2011
Introduction: Laser capture microdissection (LCM) facilitates procurement of defined cell populations for study in the context of histopathology. The morphologic assessment step in the LCM procedure is time consuming and tedious, thus restricting the ...
Jason Hipp, Jerome Cheng, Jeffrey C Hanson, Wusheng Yan, Phil Taylor, Nan Hu, Jaime Rodriguez-Canales, Jennifer Hipp, Michael A Tangrea, Michael R Emmert-Buck, Ulysses Balis   +10 more
doaj   +1 more source