Differential regulation of actin microfilaments by human MICAL proteins
The Drosophila melanogaster MICAL protein is essential for the neuronal growth cone machinery that functions through plexin- and semaphorin-mediated axonal signaling. Drosophila MICAL is also involved in regulating myofilament organization and synaptic structures, and serves as an actin disassembly factor downstream of plexin-mediated axonal repulsion.
Sai Srinivas Panapakkam, Giridharan +3 more
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Regulation of Apicomplexan Microfilament Dynamics by a Minimal Set of Actin‐Binding Proteins [PDF]
Efficient and rapid host cell invasion is a prerequisite for an intracellular parasitic life style. Pathogens typically induce receptor‐mediated endocytosis and hijack the force‐transducing system of a host cell to gain access to a replication‐competent niche.
Herwig, Schüler, Kai, Matuschewski
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RhoA-GTPase Modulates Neurite Outgrowth by Regulating the Expression of Spastin and p60-Katanin
RhoA-GTPase (RhoA) is widely regarded as a key molecular switch to inhibit neurite outgrowth by rigidifying the actin cytoskeleton. However, during neurite outgrowth, whether and how microtubule dynamics are regulated by RhoA remains to be elucidated ...
Dandan Tan +9 more
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The human mutant cardiac α-actins p.A295S or p.R312H and p.E361G, correlated with hypertrophic or dilated cardiomyopathy, respectively, were expressed by the baculovirus/Sf21 insect cell system and purified to homogeneity.
Constanze Erdmann +10 more
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Molecular imaging of membrane proteins and microfilaments using atomic force microscopy [PDF]
Atomic force microscopy (AFM) is an emerging technique for a variety of uses involving the analysis of cells. AFM is widely applied to obtain information about both cellular structural and subcellular events. In particular, a variety of investigations into membrane proteins and microfilaments were performed with AFM.
Se-Hui, Jung +4 more
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Identification of key genes and validation of key gene aquaporin 1 on Wilms’ tumor metastasis [PDF]
Background Wilms’ tumor (WT) is one of the most common solid tumors in children with unsatisfactory prognosis, but few molecular prognostic markers have been discovered for it. Many genes are associated with the occurrence and prognosis of WT. This study
Hong Liu +5 more
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Synthesis of proto-oncogene proteins and cyclins depends on intact microfilaments
It is well established that microfilament disintegration by cytochalasin D (CD) as well as latrunculin (LAT)-A and LAT-B causes an inhibition of S phase entry of various nontransformed cell lines. Our experiments extended these observations to human embryonal diploid fibroblasts (Wi-38). To investigate the question whether this stop of DNA synthesis is
M, Fasshauer, M, Iwig, D, Glaesser
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The exocyst at the interface between cytoskeleton and membranes in eukaryotic cells
Delivery and final fusion of the secretory vesicles with the relevant target membrane are hierarchically organized and reciprocally interconnected multi-step processes involving not only specific protein-protein interactions, but also specific protein ...
Lukas eSynek +4 more
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The Arabidopsis thaliana Class II Formin FH13 Modulates Pollen Tube Growth
Formins are a large, evolutionarily conserved family of actin-nucleating proteins with additional roles in regulating microfilament, microtubule, and membrane dynamics.
Eva Kollárová +2 more
doaj +1 more source
Baculovirus Expression and Functional Analysis of Vpa2 Proteins from Bacillus thuringiensis
The mode of action underlying the insecticidal activity of the Bacillus thuringiensis (Bt) binary pesticidal protein Vpb/Vpa (formerly Vip1/Vip2) is uncertain. In this study, three recombinant baculoviruses were constructed using Bac-to-Bac technology to
Oihane Simón +4 more
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