Results 141 to 150 of about 42,496 (156)

A human cell-based reporter detects microhomology-mediated end joining [PDF]

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2012
DNA double-strand breaks (DSBs) are most often repaired by two pathways in mammalian cells, homologous recombination or non-homologous end joining. Biochemical and genetic studies showed that DSBs can also be joined via microhomology-mediated end joining (MHEJ), which is always mutagenic and may result in diseases, such as cancer.
Yanguo Liu, Changshun Shao, Jay A Tischfield   +2 more
exaly   +3 more sources

Some of the next articles are maybe not open access.

Related searches:

Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus

Fungal Genetics and Biology, 2016
Filamentous fungi have a dominant nonhomologous-end joining (NHEJ) DNA repair pathway, which results in the majority of transformed progenies having random heterologous insertion mutagenesis. Thus, lack of a versatile genome-editing tool prevents us from carrying out precise genome editing to explore the mechanism of pathogenesis.
Chi Zhang, Ling Lu
exaly   +3 more sources

Involvement of Ku80 in microhomology-mediated end joining for DNA double-strand breaks in vivo

DNA Repair, 2007
Mammalian cells have an activity of mutagenic repair for DNA double-strand breaks (DSBs), microhomology-mediated end joining (MMEJ), in which DNA ends are joined via microhomologous sequences flanking the breakpoint. MMEJ has been indicated to be undertaken without Ku proteins, which are essential factors for non-homologous end joining (NHEJ).
Masanori Sato, Kiyoshi Yamaoka, Kazumi Suzukawa   +2 more
exaly   +3 more sources

The Role of Non-Homologous End Joining and Microhomology-Mediated End Joining in Chromosomal Rearrangements

Biochemistry (Moscow)
Double-strand DNA break (DSB) repair mechanisms vary in their ability to prevent errors during end joining. The joining of DSBs on different chromosomes can result in translocations, potentially leading to tumorigenesis. This review examines the main mechanisms of DSB repair and factors influencing their selection, as well as contribution of these ...
Nikolai A, Lomov, Nikolai A, Nikolaev, Vladimir S, Viushkov, Mikhail A, Rubtsov   +3 more
openaire   +2 more sources

Efficient ligase 3-dependent microhomology-mediated end joining repair of DNA double-strand breaks in zebrafish embryos

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2015
DNA double-strand break (DSB) repair is of considerable importance for genomic integrity. Homologous recombination (HR) and non-homologous end joining (NHEJ) are considered as two major mechanistically distinct pathways involved in repairing DSBs.
Hua-Lin Wang, Zuo-Yan Zhu, Yonghua Sun   +2 more
exaly   +2 more sources

The Role of Microhomology-Mediated End Joining (MMEJ) at Dysfunctional Telomeres

Cold Spring Harbor Perspectives in Biology
DNA double-strand break (DSB) repair pathways are crucial for maintaining genome stability and cell viability. However, these pathways can mistakenly recognize chromosome ends as DNA breaks, leading to adverse outcomes such as telomere fusions and malignant transformation.
David, Billing, Agnel, Sfeir
openaire   +2 more sources

A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair

2018
Following completion of the genome sequences of Xenopus tropicalis and X. laevis, gene targeting techniques have become increasingly important for the further development of Xenopus research in the life sciences. Gene knockout using programmable nucleases, such as TALEN and CRISPR/Cas9, has reached a level whereby we can readily and routinely perform ...
Ken-Ich T, Suzuki, Yuto, Sakane, Miyuki, Suzuki, Takashi, Yamamoto   +3 more
openaire   +2 more sources

CRISPR-Cas-based Microhomology-Mediated End Joining for Precise Gene Replacement in Plant

2022
Abstract Precise gene or allele replacement is a desirable technology, but implementing it in plants remains challenging. CRISPR-Cas-based approaches, such as gene targeting (GT) and prime editing (PE), have opened up new possibilities for precise gene replacement in plants.
Tien Van Vu, Gah-Hyun Lim, Seung Hee Choi, Ju Yeon Moon, Ngan Thi Nguyen, Swati Das, Mil Thi Tran, Yeon Woo Sung, Jihae Kim, Young Jong Song, Suk Weon Kim, Jae Cheol Jeong, Jae-Yean Kim   +12 more
openaire   +1 more source

cGAS restricts PARP1-mediated microhomology-mediated end joining by suppressing poly-ADP-ribosylation

Cell Death & Differentiation
Repair of DNA double-strand breaks (DSBs) is essential for cells to maintain genome stability and cell survival. While cyclic GMP-AMP synthase (cGAS) is best known for its role in innate immunity, emerging evidence reveals that it plays regulatory roles in DNA damage response.
Haibo Yang, Boya Gao, Bo Wu, Wenjing Li, Xueping Zhu, Laiyee Phoon, Jing Luo, Fu Gui, Weixing Zhao, Li Jia, Li Lan   +10 more
openaire   +2 more sources

Author Correction: Polλ promotes microhomology-mediated end-joining

Nature Structural & Molecular Biology, 2023
Gurushankar Chandramouly, Joonas Jamsen, Nikita Borisonnik, Mrityunjay Tyagi, Marissa L. Calbert, Taylor Tredinnick, Ahmet Y. Ozdemir, Tatiana Kent, Elena V. Demidova, Sanjeevani Arora, Samuel H. Wilson, Richard T. Pomerantz   +11 more
openaire   +1 more source